Apparatus for accessing a sealed container

• US 5,935,523 A
• Filed: 05/29/1997
• Issued: 08/10/1999
• Est. Priority Date: 05/29/1997
• Status: Expired due to Term

• Current Assignee: Medical Laboratory Automation Incorporated
• Original Assignee: Medical Laboratory Automation Incorporated
• Inventors: Lopez, Hugo, McCandless, William, Lovette, Spencer, Desanto, Art
• Primary Examiner(s): Le, Long V.
• Application Number:
  US08/864,829
• Time in Patent Office: 803 Days
• Field of Search: 422/63, 422/65, 422/100, 422/104, 436/43, 436/47, 436/49, 436/54, 436/174, 436/180, 73/864.22, 73/864.24, 73/864.25, 141/276, 141/330, 141/329
• US Class Current: 422/510
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums
• CPC Class Codes: G01N 35/109 with two horizontal degrees of freedom
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure

Abstract

A method and apparatus are provided for accessing a sealed container, and in particular a sealed container which is at a pressure other than atmospheric and which generally contains blood or other bodily fluid. A piercer and a probe are provided which are mounted to an XYZ positioning mechanism and are controlled such that the piercer passes through a stopper of the container, forming a cut therein through which the probe may subsequently enter the container. A foot mechanism is provided which is held against the stopper or seal for the container during piercer or probe removal for stripping purposes and through which the probe and piercer pass, the size and shape of the foot being such that, for both piercing and probing operations, it is over only a single container. The piercer and probe are operated such that the one not being used is in each instance in a raised position relative to the one being used. A novel lubrication station is provided in which the piercer is dipped prior to use to facilitate the piercing operation, to coat the cut and to minimize stopper debris. A wash station is also provided.

Claims

1. Apparatus for accessing fluid in a container which container is sealed by a piercable seal comprising:

a piercer;

a probe having a hollow tube into which said fluid is to be drawn;

a first mechanism for moving both the piercer and the probe in the Z direction perpendicular to said seal, at least one of said piercer and probe not normally being attached to move with the first mechanism, but being selectively attachable to move therewith;

a second mechanism which moves the first mechanism, including the piercer and the probe in X and Y directions substantially perpendicular to the Z direction; and

controls for operating said first and second mechanisms to have said piercer pierce the seal of a container while the probe is maintained a selected distance above the seal to withdraw the piercer from the seal, leaving a cut, and to then have the probe pass through said cut in the seal to enter said fluid while the piercer is maintained at a selected distance above the seal.

2. Apparatus as claimed in claim 1 including a foot through which said piercer and probe pass, said foot being at the bottom of a foot mechanism which is mounted to normally move with said first mechanism in the Z direction, said controls locking said foot mechanism against Z direction movement when the foot is lowered into contact with the seal of a container and releasing the foot mechanism to again move with the first mechanism when the first mechanism has risen to a predetermined position.

3. Apparatus as claimed in claim 2 wherein there are a plurality of said containers to be accessed which containers are mounted adjacent each other with a selected space therebetween, and wherein the foot has a lower seal-contacting surface of a size and shape such that said lower surface contacts only the seal for the container being accessed and does not contact the seal for an adjacent container when the foot is lowered for the piercing of the seal by the piercer and when the foot is lowered for entry of the probe through the seal.

4. Apparatus as claimed in claim 2 wherein said foot mechanism and at least one of said piercer and said probe are normally moved downward in the Z direction by gravity, such downward movement being restrained by detents limiting how far below the first mechanism they may fall, a first locking component operated by said controls for locking said foot mechanism against Z direction movement under selected conditions, and a second locking component operated by said controls under selected conditions for locking the at least one of said piercer and said probe to the first mechanism to move therewith in the Z direction.

5. Apparatus as claimed in claim 4 wherein said piercer normally rests within, and is detented against independent downward movement by, said foot, and wherein when said second locking component locks the piercer to the first mechanism, the first mechanism moves the piercer downward and upward through the foot.

6. Apparatus as claimed in claim 1 including a lubrication station, said controls operating said first and second mechanisms to position the piercer over and to dip the piercer into the lubrication station before moving the piercer to pierce said seal.

7. Apparatus as claimed in claim 6 wherein said lubrication station includes means for controlling the depth to which said piercer is lubricated.

8. Apparatus as claimed in claim 7 wherein said lubricating station includes means for removing excess lubricant from the piercer before the piercer is moved to pierce the seal.

9. Apparatus as claimed in claim 1 wherein said piercer has a plurality of vent holes circunferentially positioned above its tip and a channel leading from the vent holes out of the piercer;

and including a suction source connected to said channel, said controls operating said suction source to apply suction to the vent holes from a time before the piercer pierces the seal to a time after the piercer is removed from the seal.

10. Apparatus as claimed in claim 9 wherein said piercer, including said vent holes, have smooth rounded edges to minimize abrasion of the seal as the piercer is passed therethrough.

11. Apparatus as claimed in claim 1 wherein said probe is part of a liquid level detection mechanism, and wherein said controls are operative in response to an output from said liquid level detection mechanism before the probe has advanced sufficiently into the container to contact fluid for aborting accessing fluid from the container by the probe.

12. Apparatus as claimed in claim 1 wherein said probe is part of a liquid level detection mechanism, wherein said probe has vent holes a selected distance above its tip through which pressure inside and outside the container are equalized when the probe enters the container, and wherein said controls operate with said liquid level detection mechanism to control said first mechanism so that the probe enters fluid in the container to a depth between the vent holes and the probe tip and to lower the probe as fluid is removed by the probe to maintain the depth of the probe in the fluid within such range.

13. Apparatus as claimed in claim 1 including a wash station for said piercer and probe, said controls operating the first mechanism and the second mechanism to move the piercer and probe over and to immerse the piercer and probe in the wash station when accessing operations for a container have been completed.

14. Apparatus as claimed in claim 13 wherein each of said piercer and probe has vent holes leading to a channel therein, and including an air pressure source connected to each said channel, said controls operating each said air pressure source to blow air out through said vent holes when the piercer and probe are immersed in the wash station, thereby keeping wash fluid out of the holes and channels.

15. Apparatus as claimed in claim 13 wherein said wash station has a separate well for the piercer and the probe, and wherein said controls cause a washing fluid to flow through each well during a first part of a wash cycle to wash the element therein, and cause air to be flowed through the well for at least the probe for a second part of the wash cycle to dry the corresponding element.

16. Apparatus for accessing fluid in a container sealed by a piercable seal comprising:

a piercer;

a probe having a hollow tube into which said fluid is to be drawn;

a first mechanism for moving both the piercer and the probe in the Z direction perpendicular to said seal;

a second mechanism which moves the first mechanism, including the piercer and probe in X and Y directions substantially perpendicular to the Z direction;

a foot through which said piercer and probe independently pass, said foot being at the bottom of a foot mechanism which is mounted to normally move with said first mechanism in the Z direction and with said second mechanism in the X and Y directions; and

controls for operating said first and second mechanisms to lower said foot into contact with the seal, only said piercer passing through the foot to pierce the seal of a container, to withdraw the piercer from the seal, leaving a cut in the seal, and to have said foot again brought into contact with said seal, only the probe passing through said cut in the seal to enter said fluid, said controls locking said foot mechanism against Z direction movement each time the foot is lowered into contact with the seal of a container and releasing the foot mechanism to again move with the first mechanism each time the first mechanism has risen to a predetermined position.

17. Apparatus as claimed in claim 16 wherein there are a plurality of said containers to be accessed, which containers are mounted adjacent each other with a selected space therebetween, and wherein the foot has a lower seal-contacting surface of a size and shape such that said lower surface contacts only the seal for the container being accessed and does not contact the seal for an adjacent container when the foot is lowered for the piercing of the seal by the piercer and when the foot is lowered for entry of the probe through the seal.

18. Apparatus as claimed in claim 16 wherein said foot mechanism and at least one of said piercer and said probe are normally moved downward in the Z direction by gravity, such downward movement being restrained by detents limiting how far below the first mechanism they may fall, a first locking component operated by said controls for locking said foot mechanism against Z direction movement under selected conditions, and a second locking component operated by said controls under selected condition for locking the at least one of said piercer and said probe to the first mechanism to move therewith in the Z direction.

19. Apparatus as claimed in claim 18 wherein said piercer normally rests within, and is detented against independent downward movement by, said foot, and wherein when said second locking component locks the piercer to the first mechanism, the first mechanism moving the piercer downward and upward through the foot.

20. Apparatus for accessing fluid in a container sealed by a piercable seal comprising:

a piercer;

a probe having a hollow tube into which said fluid is to be drawn;

a first mechanism for moving both the piercer and the probe in the Z direction perpendicular to said seal;

a second mechanism which moves the first mechanism, including the piercer and probe in X and Y directions substantially perpendicular to the Z direction;

a lubrication station; and

controls for operating said first and second mechanisms to have said piercer pierce the seal of a container, to withdraw the piercer from the seal, leaving a cut in the seal, and to then have the probe pass through said cut in the seal to enter said fluid, the controls operating said first and second mechanism to position the piercer over and to dip the piercer into the lubrication station before moving the piercer to pierce said seal.

21. Apparatus as claimed in claim 20 wherein said lubrication station includes means for controlling the depth to which said piercer is lubricated.

22. Apparatus as claimed in claim 20 wherein said lubrication station includes means for removing excess lubricant from the piercer before the piercer is moved to pierce the seal.

23. Apparatus for accessing fluid in a container sealed by a piercable seal comprising:

a piercer having a plurality of vent holes circumferentially positioned above its tip and a channel leading from the vent holes out of the piercer;

a suction source connected to said channel;

a probe having a hollow tube into which said fluid is to be drawn;

a first mechanism for moving both the piercer and the probe in the Z direction perpendicular to said seal;

a second mechanism which moves the first mechanism, including the piercer and probe in X and Y directions substantially perpendicular to the Z direction; and

controls for operating said first and second mechanisms to have said piercer pierce the seal of a container, to withdraw the piercer from the seal, leaving a cut in the seal, and to then have the probe pass through said cut in the seal to enter said fluid, the controls operating said suction source to apply suction to the vent holes from a time before the piercer pierces the seal to a time after the piercer is removed from the seal.

24. Apparatus as claimed in claim 23 wherein said piercer, including said vent holes, have smooth rounded edges to minimize abrasion of the seal as the piercer is passed therethrough.

25. Apparatus for accessing fluid in a container which container is sealed by a piercable seal comprising:

a tipped component for piercing said seal;

a first mechanism for moving the component in a Z direction substantially perpendicular to said seal, said component not normally being attached to move with the first mechanism, but being selectively attachable to move therewith;

a foot through which said component passes, said foot being at the bottom of a foot mechanism which is mounted to normally move with said first mechanism in the Z direction;

a second mechanism which moves the first mechanism, including the component and the foot, in at least one direction substantially perpendicular to the Z direction; and

controls for operating said second mechanism to position said component over the seal of a container, for operating the first mechanism to then lower the component to pass through the seal, and for subsequently operating the first mechanism to raise the component through the seal to remove it from the container, said controls locking said foot mechanism to prevent further Z direction movement with the first mechanism when the foot is lowered into contact with the seal of a container, and releasing the foot mechanism to again move with the first mechanism when the first mechanism has risen to a predetermined position sufficient to fully remove the component from the container, including the seal, the locked foot mechanism facilitating striping of the component from the seal.

26. Apparatus as claimed in claim 25 wherein there are a plurality of said containers to be accessed which containers are mounted adjacent each other with a selected space therebetween, and wherein the foot has a seal-contacting surface of a size and shape such that said surface contacts only the seal for the container being accessed and does not contact the seal for an adjacent container when the foot is lowered for entry of the component through the seal.

27. Apparatus as claimed in claim 26 wherein said foot mechanism and said component are normally moved downward in the Z direction by gravity, such downward movement being restrained by detents limiting how far below the first mechanism they may fall, a first locking component operated by said controls for locking said foot mechanism against Z direction movement under selected conditions, and a second locking component operated by said controls under selected conditions for locking the component to the first mechanism to move therewith in the Z direction.