Holder for fluorometric samples

• US 5,935,524 A
• Filed: 04/21/1997
• Issued: 08/10/1999
• Est. Priority Date: 05/07/1996
• Status: Expired due to Term

• Current Assignee: Qualicon Diagnostics LLC
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Bass, Jay Kevin, Regester, David James
• Primary Examiner(s): Pyon, Harold Y.
• Application Number:
  US08/844,742
• Time in Patent Office: 841 Days
• Field of Search: 422/50, 422/52, 422/55, 422/58, 422/82.05, 422/102, 422/103, 422/104
• US Class Current: 422/562
• CPC Class Codes: B01L 9/06 Test-tube stands; Test-tube holders
• CPC Class Codes: G01N 21/01 Arrangements or apparatus for facilitating the optical investigation
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters

Abstract

A holder for fluorometric analysis of PCR reaction tubes comprises a longitudinal block, the block being sized to fit within a fluorometer; a cavity disposed along a longitudinal axis of the block; at least one test well within the cavity, the test well having an internal wall sized to closely receive and maintain intimate contact with a substantial portion of a single PCR reaction tube therein; the internal wall having at least one optical port therein; and a cover movably connected to the block.

Claims

1. A holder for fluorometric analysis of PCR reaction tubes, the holder comprising:

a longitudinal block having at least one test well therein, said block being sized to fit within a fluorometer;

a longitudinal cavity disposed along a longitudinal axis of the block;

at least one test well generally centrally located within the longitudinal cavity, said test well having an internal wall sized to closely receive and maintain intimate contact with a substantial portion of a single PCR reaction tube therein;

said internal wall having at least one optical port herein; and

a cover movably connected to said block.

2. The holder of claim 1, wherein said cover comprises means for biasing a PCR tube into intimate contact with the internal wall of said at least one test well.

3. The holder of claim 2, wherein said biasing means comprises a spring affixed to an inner surface of said cover.

4. The holder of claim 1, wherein said at least one optical port comprises an aperture in said internal wall adapted to receive and hold an optical fiber therein.

5. The holder of claim 1, further comprising a pair of longitudinal rails positioned one on either side of the test well and the longitudinal cavity, said rails, together with an upper surface of the block defining a sliding surface for a strip of PCR reaction tubes and wherein said rails comprise a light barrier and a friction seal for the cover.