DNA molecules encoding mammalian cerberus-like proteins

• US 5,935,852 A
• Filed: 07/03/1997
• Issued: 08/10/1999
• Est. Priority Date: 07/03/1997
• Status: Expired due to Term

• Current Assignee: Genetics Institute LLC (Pfizer Inc.)
• Original Assignee: Genetics Institute LLC (Pfizer Inc.)
• Inventors: Follettie, Maximillian, DeRobertis, Edward M.
• Primary Examiner(s): DUFFY, PATRICIA ANN
• Application Number:
  US08/887,997
• Time in Patent Office: 768 Days
• Field of Search: 435/6, 435/69.1, 435/70.1, 435/71.1, 435/71.2, 435/91.1, 435/91.4, 435/325, 435/357, 435/358, 435/366, 435/252.3, 435/252.33, 435/254.11, 435/320.1, 514/44, 536/23.1, 536/23.5, 536/24.31, 536/24.33, 536/24.5, 536/25.3, 530/300, 530/350
• US Class Current: 435/325
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 1/18 for pancreatic disorders, e.g. pancreatic enzymes
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 13/12 of the kidneys
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 19/10 for osteoporosis
• CPC Class Codes: A61P 21/00 Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/16 Anti-Parkinson drugs
• CPC Class Codes: A61P 25/28 for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C07K 14/475 Growth factors; Growth regulators

Abstract

DNA molecules are disclosed which encode mammalian Cerberus-Like Proteins.

Claims

1. An isolated DNA molecule comprising a DNA sequence starting at nucleotide #58, 109, 112, 115, 118, 121, 124, 127, 130, 178, 313, 316, 319, 322, 325, 328 or 541 of SEQ ID NO:

1 and ending at nucleotide #780 or 873 of SEQ ID NO;

1.

2. An isolated DNA molecule, comprising a DNA sequence fully complementary to the DNA sequence of claim 1.

3. A vector comprising the DNA sequence of claim 1 in operative association with an expression control sequence therefore.

4. An isolated host cell transformed with the vector of claim 3.

5. An isolated DNA molecule comprising a DNA sequence encoding amino acids starting at amino acid #1, 18 to 25, 41, 85 to 91, or 162 of SEQ ID NO:

2 and ending at amino acid #241 or 272 of SEQ ID NO;

2.

6. An isolated DNA molecule, comprising a DNA sequence fully complementary to the DNA sequence of claim 5.

7. A vector comprising the DNA sequence of claim 5 in operative association with an expression control sequence therefore.

8. An isolated host cell transformed with the vector of claim 7.

9. An isolated DNA molecule comprising a DNA sequence starting at nucleotide #325 of SEQ ID NO:

1 and ending at nucleotide #873 of SEQ ID NO;

1.

10. An isolated DNA molecule, comprising a DNA sequence fully complementary to the DNA sequence of claim 9.

11. A vector comprising the DNA sequence of claim 9 in operative association with an expression control sequence therefore.

12. An isolated host cell transformed with the vector of claim 11.

13. An isolated DNA molecule comprising a DNA sequence encoding amino acids starting at amino acid #90 of SEQ ID NO:

2 and ending at amino acid #272 of SEQ ID NO;

2.

14. An isolated DNA molecule, comprising a DNA sequence fully complementary to the DNA sequence of claim 13.

15. A vector comprising the DNA sequence of claim 13 in operative association with an expression control sequence therefore.

16. An isolated host cell transformed with the vector of claim 15.