Microplate assembly

• US 5,939,024 A
• Filed: 12/23/1997
• Issued: 08/17/1999
• Est. Priority Date: 12/23/1997
• Status: Expired due to Term

• Current Assignee: Packard Instrument Co., Inc. (Revvity, Inc.)
• Original Assignee: Packard Instrument Co., Inc. (Revvity, Inc.)
• Inventors: Robertson, James E.
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US08/997,182
• Time in Patent Office: 602 Days
• Field of Search: 422/99, 422/101, 422/102, 422/104, 356/246, 356/440, 435/288.4, 435/288.5
• US Class Current: 422/534
• CPC Class Codes: B01L 3/50255 Multi-well filtration

Abstract

A microplate assembly for use in analyzing samples captured on a filter medium comprises a holding tray and a collimator having multiple sample wells. These elements are generally rectangular in shape and are sized to stack on top of one another. The filter medium is positioned within the holding tray and the holding tray is positioned within the collimator with the filter medium positioned beneath the collimator. To prepare samples in the microplate assembly for analysis, the samples are captured on the filter medium and the filter medium is placed in the holding tray. After adding scintillation cocktail or luminescent substrate to the filter medium, the collimator is placed over the holding tray with the filter medium positioned between the collimator and the holding tray and the samples disposed in the sample wells. The holding tray, the filter medium and the collimator are provided with complementary keyed corners to facilitate alignment of these elements relative to one another. The wells of the collimator include respective lower rims protruding into the filter medium to minimize crosstalk through the filter medium. The collimator and holding tray include structure for defining multiple positions of engagement with each other for accommodating filters of different thicknesses.

Claims

1. A microplate assembly for use in analyzing samples captured on a filter medium having an upper and lower surface, comprising:

a holding tray having a recessed surface and side walls connected to said recessed surface, said holding tray receiving therein the filter medium with the lower surface adjacent to said recessed surface of said holding tray;

a collimator abutting the upper surface of the filter medium, said collimator being disposed substantially parallel to said recessed surface of said holding tray, said collimator having wells formed therein for surrounding the samples on the filter medium; and

variably relatively positionable cooperating engagement means formed on said collimator and said holding tray for maintaining said collimator and said holding tray in an assembled condition in one of at least two relative positions, dependent upon the thickness of said filter medium, with said filter medium engaged between the wells of said collimator and the recessed surface of said holding tray, so as to accommodate filter mediums over a range of thicknesses.

2. The microplate assembly of claim 1, wherein said collimator has side walls dimensioned for surrounding said holding tray side walls and wherein said cooperating engagement means comprises a plurality of sets of complementary facing projecting surfaces on said collimator side walls and said holding tray side walls respectively, the projecting surfaces on said collimator side wall and said holding tray side walls defining multiple relative positions for engagement with each other.

3. The microplate assembly of claim 2 wherein said projecting surfaces comprise at least two sets of inwardly projecting ridges formed on opposite ones of the side walls of said collimator and, at least two sets of outwardly projecting fingers, each comprising at least two fingers of different length, formed on opposite ones of the side walls of said holding tray and positioned for alignment with said ridges.

4. The microplate assembly of claim 3 wherein said fingers project beyond peripheral edge parts of said side walls of said holding tray so as to resiliently deflect to allow passage of said ridges and thereafter, at least one of said fingers resiliently returning to engage a trailing surface of said ridge.

5. The microplate assembly of claim 3 wherein said fingers have ramped lead-in surfaces for facilitating initial movement thereof past said ridges.

6. The microplate assembly of claim 3 wherein said ridges have ramped lead-in surfaces for facilitating initial movement of the fingers thereby.

7. The microplate assembly according to claim 3 wherein each of said sets of fingers is three in number, each of said three fingers being of a different length from the other two fingers of the same set and of like length to one of the fingers in each of the other sets.

8. The microplate assembly of claim 3 wherein said sets of projecting ridges and fingers are located respectively at longitudinally opposite ones of said side walls of the collimator and holding tray.

9. The microplate assembly of claim 8 and further including at least one additional raised ridge and complementary finger on each of the remaining laterally opposed side walls of said collimator and said holding tray.

10. The microplate assembly of claim 1 and further including projecting gripping means on said holding tray for facilitating engagement thereof for disassembly from said collimator.

11. The microplate assembly of claim 1, wherein said holding tray and said collimator include complementary keyed corners for aligning said holding tray and said collimator relative to one another.

12. The microplate assembly of claim 1, wherein said side walls and said recessed surface of said holding tray form a generally rectangular compartment for receiving the filter medium therein.

13. The microplate assembly of claim 1, wherein said wells of said collimator are arranged in a matrix corresponding to the arrangement of the samples on the filter medium so that each of said wells surrounds a separate sample.

14. The microplate assembly of claim 1, wherein said wells of said collimator include respective lower rims protruding from the respective lower peripheries of said wells into the filter medium to minimize crosstalk through the filter medium.

15. The microplate assembly of claim 1, wherein each of said wells of said collimator includes an upper rim formed around the upper periphery thereof to minimize crosstalk between said wells.

16. The microplate assembly of claim 1, wherein said holding tray and said collimator are reusable.

17. The microplate assembly of claim 1, wherein the filter medium is cut to the size and shape of the recessed surface of said holding tray so that the filter medium fits snugly within the recessed surface of said holding tray.

18. The microplate assembly of claim 1, wherein said recessed surface of said holding tray is provided with anti-reflective elements to reduce crosstalk between said samples.

19. The microplate assembly of claim 18, wherein said anti-reflective elements comprise a plurality of dark circles inscribed on said recessed surface of said holding tray alignable with the wells of said collimator.

20. The microplate assembly of claim 1 wherein said recessed surface of said holding tray is reflective.

21. The microplate assembly of claim 20, wherein said recessed surface of said holding tray is provided with anti-reflective elements to reduce crosstalk between said samples.

22. The microplate assembly of claim 21, wherein said anti-reflective elements comprise a plurality of dark circles inscribed on said recessed surface of said holding tray alignable with the wells of said collimator.