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Microfluidic method for nucleic acid amplification

  • US 5,939,291 A
  • Filed: 06/14/1996
  • Issued: 08/17/1999
  • Est. Priority Date: 06/14/1996
  • Status: Expired due to Fees
First Claim
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1. An amplification device for target nucleic acids comprising a reaction chamber and at least one supply chamber, wherein amplicons complementary to a target nucleic acid segment of the target nucleic acid are generated in a reaction chamber within the device and form duplex amplicons between complementary amplicons, and wherein denaturation of the duplex amplicons is effected in the reaction chamber by a chemical denaturant or an electrostatic process wherein the device further comprises:

  • an electric field generating device effective to create a field at a reaction chamber of the device such that the strands of amplicons in that chamber separate, wherein the amplicons are each formed from first primer and a second primer, where the first primers have one charge and the second primers have the opposite charge.

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