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Thermostable DNA polymerases having reduced discrimination against ribo-NTPs

  • US 5,939,292 A
  • Filed: 08/05/1997
  • Issued: 08/17/1999
  • Est. Priority Date: 08/06/1996
  • Status: Expired due to Term
First Claim
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1. A recombinant thermostable DNA polymerase that is a mutant form of a naturally occurring thermostable DNA polymerase, wherein said naturally occurring thermostable DNA polymerase has an amino acid sequence comprising amino acid sequence motif SerGlnIleGluLeuArgXaa (SEQ ID NO:

  • 2), wherein "Xaa" at position 7 of said sequence motif is a valine residue (Val) or an isoleucine residue (Ile);

    wherein said mutant form has been modified to contain an amino acid other than glutamic acid (Glu) at position 4 of said sequence motif; and

    wherein said mutant form possesses reduced discrimination against incorporation of an unconventional nucleotide in comparison to said naturally occurring thermostable DNA polymerase.

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