Method for purifying GBS toxin/CM101
First Claim
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1. A method of purifying a polysaccharide toxin from group B β
- -hemolytic Streptococcus (GBS) bacteria, which method comprises;
(a) contacting an aqueous mixture containing the toxin with a hydrophobic interaction chromatography (HIC) resin; and
(b) separating the toxin from the HIC resin.
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Abstract
A method for purifying a polysaccharide from group B β-hemolytic Streptococcus (GBS) bacteria includes contacting a bacterial fermentation stock with a hydrophobic interaction chromatography (HIC) resin. Additional steps may include a phenol/saline extraction and an ion exchange chromatography. The method results in a product having very high purity. The product of the purification provides a composition which is highly useful in both research and therapeutic settings.
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21 Claims
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1. A method of purifying a polysaccharide toxin from group B β
- -hemolytic Streptococcus (GBS) bacteria, which method comprises;
(a) contacting an aqueous mixture containing the toxin with a hydrophobic interaction chromatography (HIC) resin; and (b) separating the toxin from the HIC resin. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
- -hemolytic Streptococcus (GBS) bacteria, which method comprises;
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18. A method of purifying a polysaccharide toxin from group B β
- -hemolytic Streptococcus (GBS) bacteria comprising;
(1) applying a source of GBS bacterial toxin to a hydrophic interaction chromatography (HIC) column, (2) eluting the HIC column to obtain an HIC eluate including the toxin, (3) extracting the HIC eluate with a combination of phenol and an aqueous solution to form an aqueous phase including the toxin, (4) applying the aqueous phase to an ion exchange column, and (5) eluting the ion exchange column to obtain an ion exchange eluate, wherein the ion exchange eluate comprises a substantially pure toxin. - View Dependent Claims (19, 20, 21)
- -hemolytic Streptococcus (GBS) bacteria comprising;
Specification