Transgenic swine compositions and methods
First Claim
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1. A method of isolating and purifying an embryonic stem cell culture, said method comprising:
- (a) culturing dissociated cells from a swine embryo in conditioned stem cell medium in the absence of a feeder layer, wherein the dissociated cells are obtained from a swine embryo which was developed in vitro in stem cell medium (SCM) on a feeder layer; and
(b) subculturing the culture until a stable culture with morphological features and growth parameters characteristic of an embryonic stem cell culture is established.
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Abstract
Transgenic swine, and compositions and methods for making and using same, are provided. Central to the invention are porcine (Sus scrofa) embryonic stem cell lines and methods for establishing them. Cells of such lines are transformed with exogenous genetic material of interest and then used to provide chimeric swine, which have germ cells comprising the exogenous genetic material. The chimeric swine are bred to provide transgenic swine. Transgenic swine of the invention can be used to provide human proteins or peptide hormones or can be used as xenograft donors.
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Citations
13 Claims
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1. A method of isolating and purifying an embryonic stem cell culture, said method comprising:
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(a) culturing dissociated cells from a swine embryo in conditioned stem cell medium in the absence of a feeder layer, wherein the dissociated cells are obtained from a swine embryo which was developed in vitro in stem cell medium (SCM) on a feeder layer; and (b) subculturing the culture until a stable culture with morphological features and growth parameters characteristic of an embryonic stem cell culture is established. - View Dependent Claims (2, 3)
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- 4. An isolated swine embryonic stem cell.
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10. A stable cell line derived from a culture of undifferentiated swine embryonic stem cells.
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11. A method for making a chimeric swine comprising:
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(a) introducing a cultured swine embryonic stem cell that has a first genetic complement into a swine host embryo that has a second genetic complement, to form a chimeric swine embryo; and (b) placing the chimeric swine embryo in an environment suitable for the completion of development to form a chimeric swine. - View Dependent Claims (12, 13)
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Specification