Marking of products to establish identity, source and fate

• US 5,942,444 A
• Filed: 01/27/1997
• Issued: 08/24/1999
• Est. Priority Date: 01/27/1997
• Status: Expired due to Fees

• Current Assignee: Authentix, Inc.
• Original Assignee: Biocode, Inc. (Authentix, Inc.)
• Inventors: Jenkins, Robin, Rittenburg, James H.
• Primary Examiner(s): Spiegel, Carol A.
• Application Number:
  US08/788,392
• Time in Patent Office: 939 Days
• Field of Search: 106/31.01, 208/12, 252/182.13, 427/444, 435/4, 435/7.1, 435/9, 436/518, 436/543, 436/2, 436/8, 436/20, 436/22, 436/23, 436/24, 436/29
• US Class Current: 436/518
• CPC Class Codes: C07K 1/1077 by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
• CPC Class Codes: C07K 1/13 Labelling of peptides
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 2563/185 Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template
• CPC Class Codes: G01N 33/2882 Markers marking of fuels C10L1/003
• CPC Class Codes: G09F 3/00 Labels, tag tickets, or similar identification or indication means medals or badges A44C3/00; making labels B31D1/02; sheets temporarily attached together B42F; labelling B65C; labels on record carriers G11B23/38, G11B23/40; Seals; Postage or like stamps
• CPC Class Codes: Y10T 436/10 Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing

Abstract

In general, the invention features a method of marking a product for identification in which a marker, composed of a print molecule, print molecule analogue, or molecularly imprinted molecule, are added to the product and subsequently measured in a specific binding assay.

Claims

1. A method of marking a liquid, organic, non-aqueous product for identification and then detecting said marked product, said method comprising

a) associating a marker with the product, said marker being inert and non-deleterious with respect to the product and not already associated with the product, said marker being either (i) a print molecule or (ii) a molecularly imprinted, polymeric anti-print molecule which specifically binds to said print molecule, wherein the polymeric anti-print molecule is a synthetic, non-naturally-occurring polymer,

b) forming a specific binding complex between said marker and either (i) said molecularly imprinted, polymeric anti-print molecule or (ii) said print molecule, respectively;

and

c) detecting said marked product by detecting said specific binding complex, wherein said detecting is carried out directly in a sample of said product without prior extraction into an aqueous phase.

2. The method of claim 1 wherein said molecularly imprinted, polymeric anti-print molecule comprises a homo- or hetero-polymer of functional monomers selected from the group consisting of acrylic acid, methacrylic acid, ethylene dimethacrylate, vinyl pyridine, sulfonic acid, vinylimidazole, and itaconic acid.

3. The method of claim 1 wherein said print molecule is a compound containing a metal ion selected from the group consisting of Cu²⁺, Cd²⁺, Fe²⁺, Co²⁺, Zn²⁺, Ni²⁺, and Mg²⁺.

4. The method of claim 1 wherein said marker is a print molecule selected from the group consisting of dyes, fluorophores, and chemiluminescors.

5. The method of claim 1 wherein said marker is a print molecule which has a physical characteristic which is detectable in a concentration-dependent manner and said specific binding complex is detected by concentrating said print molecule and detecting said physical characteristic.

6. The method of claim 1 wherein said associating step comprises adding said marker to said product.

7. The method of claim 6 in which said adding step comprises mixing said marker with said product.

8. The method of claim 7 in which said product is a petroleum product.

9. A method of monitoring or tracing a liquid organic, non-aqueous process chemical or specialty additive, said method comprising:

(a) associating said chemical or said additive with a marker, said marker being inert and non-deleterious with respect to said chemical or additive and not already associated with said chemical or additive, said marker being either (i) a print molecule or (ii) a molecularly imprinted, polymeric anti-print molecule which specifically binds to said print molecule, wherein said polymeric anti-print molecule is a synthetic non-naturally occurring polymer;

(b) obtaining a sample from the process or product in which said chemical or additive is employed;

(c) forming a specific binding complex between said marker in said sample and said (i) molecularly imprinted, polymeric anti-print molecule or (ii) said print molecule, respectively; and

(d) measuring the concentration of said specific binding complex in said sample without extracting said sample into an aqueous phase to monitor or trace said chemical or additive.

10. The method of claim 9 wherein said chemical or additive is selected from the group consisting of a biocide, a water treatment chemical, a food additive, a plastics additive and a petroleum product additive.