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Ligation assembly and detection of polynucleotides on solid-support

  • US 5,942,609 A
  • Filed: 11/12/1998
  • Issued: 08/24/1999
  • Est. Priority Date: 11/12/1998
  • Status: Expired due to Term
First Claim
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1. A method of synthesizing a polynucleotide on a solid-support, the method comprising the steps of:

  • a. annealing one or more bridging oligonucleotides and two or more assembly oligonucleotides such that a ligatable nick site is formed between adjacent assembly oligonucleotides, wherein one of the assembly oligonucleotides is immobilized to a solid support;

    b. ligating the ligatable nick sites thereby forming an immobilized ligation product;

    c. annealing a primer to the immobilized ligation product; and

    d. extending the primer to create an immobilized, double-stranded polynucleotide.

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