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Methods and systems for performing electrophoretic molecular separations

  • US 5,948,227 A
  • Filed: 12/17/1997
  • Issued: 09/07/1999
  • Est. Priority Date: 12/17/1997
  • Status: Expired due to Term
First Claim
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1. A method of separating macromolecules by capillary electrophoresis, comprising:

  • providing a substrate comprising at least a first capillary channel disposed therein, a surface of the channel having a first surface charge associated therewith;

    filling said capillary channel with a water soluble hydrophilic polymer solution having a percent charge of from about 0.01% to about 2%, as calculated by the molar percent of charged monomer subunits to total monomer utilized in producing the polymer, the charged monomer subunits consist of monomer subunits having a charge that is the same as the first surface charge;

    introducing a sample containing the macromolecules into one end of the capillary channel and;

    applying a voltage gradient across the length of the capillary channel, whereby the macromolecules in the sample are separated in the capillary channel.

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