Methods and systems for performing electrophoretic molecular separations
First Claim
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1. A method of separating macromolecules by capillary electrophoresis, comprising:
- providing a substrate comprising at least a first capillary channel disposed therein, a surface of the channel having a first surface charge associated therewith;
filling said capillary channel with a water soluble hydrophilic polymer solution having a percent charge of from about 0.01% to about 2%, as calculated by the molar percent of charged monomer subunits to total monomer utilized in producing the polymer, the charged monomer subunits consist of monomer subunits having a charge that is the same as the first surface charge;
introducing a sample containing the macromolecules into one end of the capillary channel and;
applying a voltage gradient across the length of the capillary channel, whereby the macromolecules in the sample are separated in the capillary channel.
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Abstract
The present invention provides methods of electrophoretically separating macromolecular species, as well as compositions and systems useful in carrying out such methods. Specifically, the methods of the present invention comprise providing a substrate that has at least a first capillary channel disposed therein. The surface of the channel has a first surface charge associated therewith, and is filled with a water soluble surface adsorbing polymer solution that bears a net charge that is the same as the charge on the capillary surface.
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Citations
50 Claims
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1. A method of separating macromolecules by capillary electrophoresis, comprising:
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providing a substrate comprising at least a first capillary channel disposed therein, a surface of the channel having a first surface charge associated therewith; filling said capillary channel with a water soluble hydrophilic polymer solution having a percent charge of from about 0.01% to about 2%, as calculated by the molar percent of charged monomer subunits to total monomer utilized in producing the polymer, the charged monomer subunits consist of monomer subunits having a charge that is the same as the first surface charge; introducing a sample containing the macromolecules into one end of the capillary channel and; applying a voltage gradient across the length of the capillary channel, whereby the macromolecules in the sample are separated in the capillary channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method of separating macromolecules by capillary electrophoresis, comprising:
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providing a silica substrate having a capillary channel disposed therein, a surface of the channel having a negative surface charge associated therewith; filling said capillary channel with a water soluble hydrophilic polymer solution having a net charge of from about 0.1% to about 2%, the charge being the same as the surface charge; introducing a sample containing the macromolecules into one end of the capillary channel; and applying a voltage gradient across the length of the capillary channel, whereby the macromolecules in the sample are separated in the capillary channel.
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27. A method of preparing a walled capillary channel for use in separating macromolecules, comprising:
filling the capillary channel with a silica adsorbing polymer solution, wherein the polymer has a net charge that is the same as a net charge associated with interior surfaces of the walled capillary channel.
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28. A system for separating macromolecules by capillary electrophoresis, comprising:
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a substrate having at least a first walled capillary channel disposed therein, the channel having a net surface charge associated with interior surfaces of the channel; a solution of silica adsorbing polymer disposed in the capillary channel, the solution of polymer comprising; a molecular weight between about 1 Kd and 5,000 Kd; a net charge of between about 0.01 and 2%, the net charge being the same as the net surface charge; and a power source electrically coupled to the first capillary channel for applying a voltage gradient across the capillary channel. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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49. A system for separating nucleic acids by molecular weight, comprising:
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a silica substrate having a walled capillary channel disposed therein, the channel having a negative charge associated with interior surfaces of the channel; a solution of silica adsorbing polymer disposed in the capillary channel, the solution of polymer comprising; a molecular weight between about 1 Kd and 5,000 Kd; a net negative charge of between about 0.01 and 2%; and a power source for applying a voltage gradient across the capillary channel.
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50. A walled capillary for separating macromolecules by capillary electrophoresis, comprising:
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a capillary channel disposed in a solid substrate, interior surfaces of the capillary channel having a first net surface charge associated therewith; and a solution of silica adsorbing polymer disposed in the capillary channel, the polymer comprising; a molecular weight between about 1 Kd and about 5,000 Kd; a net charge of between about 0.01 and 2%, the net charge being the same as the first net surface charge.
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Specification