Shaping of microparticles in electric-field cages

• US 5,948,328 A
• Filed: 08/23/1996
• Issued: 09/07/1999
• Est. Priority Date: 02/24/1994
• Status: Expired due to Fees

• Current Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Original Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Inventors: Mueller, Torsten, Schnelle, Thomas, Voigt, Andreas, Fiedler, Stefan, Fuhr, Guenter, Hagedorn, Rolf, Wagner, Bernd, Glasser, Henning, Hornung, Jan
• Primary Examiner(s): MULLIS, JEFFREY C
• Application Number:
  US08/700,395
• Time in Patent Office: 1,110 Days
• Field of Search: 264/5, 264/10, 264/6, 264/15
• US Class Current: 264/5
• CPC Class Codes: B01J 13/02 Making microcapsules or microballoons for medical preparations A61K9/50
• CPC Class Codes: B01J 19/087 employing electric or magnetic energy
• CPC Class Codes: B01J 2/00 Processes or devices for granulating materials , e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic

Abstract

In the method proposed, microparticles suspended in a liquid or droplets suspended in a liquid with which they are immiscible are shaped by high-frequency electric fields in a three-dimensional electrode array of a size in the micrometer or submicrometer range and subsequently consolidated by prior art chemical bonding procedures or by physical methods. The disposition, geometry and control of the electrodes determine the shape of the particles. The particles themselves must have a conductivity and/or relative dielectric constant lower than the solution surrounding them. For some, this can be achieved only at certain frequencies in the kHz and MHz band which are determined by the passive electrical properties of the particles and the surrounding solution. The particles or droplets are repelled by the electrodes so that they are shaped in the free solution without making contact with any surface and can then be consolidated. This makes it possible to shape micrometer and submicrometer size particles of the kind required in chromatography, affinity biochemistry and medicine, as well as for filter systems.

Claims

1. Process for shaping at least one microparticle, comprising:

providing at least two spatially disposed electrodes thereby defining a reaction space fillable with a surrounding liquid, the electrodes having a width, a height and defining an interspace between the electrodes;

driving the electrodes with varying electrical voltages;

providing at least one of;

at least one small particle;

an aggregate of small particles;

a surrounding liquid with at least one small particle suspended therein;

a surrounding liquid with a dispersed phase; and

a surrounding liquid with at least one small particle and a dispersed phase, at least one of a relative dielectric constant and a specific conductivity of the small particle and the dispersed phase, respectively, at a selected varying electrical voltage of said electrodes being lower than that of said surrounding liquid;

at least partially immersing the electrodes in the surrounding liquid thereby filling the reaction space with surrounding liquid;

wherein forces due to the varying electrical voltages driving the electrodes act on one of said at least one small particle and said dispersed phase, the varying electrical voltages driving the electrodes being adjusted such that at least one of said at least one small particle suspended in said surrounding liquid and said dispersed phase is formed as a differently shaped microparticle and at least partially permanently shaped by one of cross-linking, hardening and solidifying.

2. Shaping of at least one microparticle according to claim 1, wherein the microparticle has a body that is solidified by chemical reaction induced by at least one of an optical stimulation, addition of substances and electropolymerization.

3. Shaping of at least one microparticle according to claim 2 wherein two or more different compositions react with each other thereby inducing permanent shaping.

4. Shaping of at least one microparticle according to claim 1, wherein the microparticle has a body that is solidified by a physical process.

5. Shaping of at least one microparticle according to claim 1, wherein the forces due to the varying electrical voltages driving the spatially disposed electrodes form a field in the reaction space and at least one of arranged and unarranged microparticles are assembled into an aggregate in the field of the reaction space.

6. Shaping of at least one microparticle according to claim 1, wherein the forces due to the varying electrical voltages driving the spatially disposed electrodes form a field cage and a plurality of adjacent spatially disposed electrodes are provided defining a plurality of adjacent field cages and a plurality of microparticles are formed adjacent to each other in the plurality of adjacent field cages.

7. Shaping of at least one microparticle according to claim 6, wherein the plurality of microparticles formed adjacent to each other are at least partially assembled in the field cages after addition of at least one further material.

8. Shaping of at least one microparticle according to claim 6, wherein the plurality of adjacent spatially disposed electrodes are arranged to form a channel as the reaction space and a microparticle in a form of one of a band and a chain of predeterminable length is formed in the plurality of adjacent field cages along the reaction space channel.

9. Shaping of at least one microparticle according to claim 8, wherein the reaction space is elongated for the production of band-shaped microstructures, the reaction space being defined by a plurality of electrode multipoles disposed along said reaction space, the multipoles being arranged in a plane perpendicular an extension of the elongated reaction space.

10. Shaping of at least one microparticle according to claim 9, wherein the electrode multipoles have at least two electrodes provided on at least two sides along the reaction chamber and wherein one of the width and the height of the electrodes is less than 1 μ

m.

11. Shaping of at least one microparticle according to claim 9, wherein each one of the electrode multipoles is a quadrupole.

12. Shaping of at least one microparticle according to claim 9, wherein more than two electrode multipoles are lined up in sequence along the extension of the elongated reaction space.

13. Shaping of at least one microparticle according to claim 1, wherein the varying electrical voltages produces high frequency electric fields at the electrodes in the kHz to GHz range.

14. Shaping of at least one microparticle according to claim 1, wherein at least one of the at least two spatially disposed electrodes is at least partially isolated from the surrounding liquid so that only a non-isolated portion of the at least two electrodes is in contact with the surrounding liquid.

15. Shaping of at least one microparticle according to claim 14, wherein said at least two spatially disposed electrodes are fully isolated, for coupling the electrical voltage induced forces into a solution having a low conductivity.

16. Shaping of at least one microparticle according to claim 1, wherein at least one of the spatially disposed electrodes comprises one of a semiconductor and an electrode driven by a semiconductor.

17. Shaping of at least one microparticle according to claim 1, wherein the spatially disposed electrodes are positioned in a three-dimensional arrangement thereby defining at least one feeding channel providing said reaction space.

18. Shaping of at least one microparticle according to claim 1, wherein the microparticle has a body at least partially assembled from small particles formed of a first composition and wherein second small particles formed of at least one of the first composition and a different composition are introduced into said reaction space and are at least partially assembled thereby forming an encapsulation, encapsulating the body and forming at least two layers.

19. Shaping of at least one microparticle according to claim 18, wherein the electrodes are driven with first varying electrical voltages and the microparticle is at least partially assembled from small particles formed of the first composition, and then the electrodes are driven with second varying electrical voltages when the microparticle is at least partially assembled from small particles formed of at least one of the first composition and the different composition, to shape an outer layer of the at least two layers differently from a shape of an inner one of the two layers.

20. Shaping of at least one microparticle according to claim 18, wherein third small particles comprising at least one of solid bodies, droplets, living cells and hollow spaces filled with gas, are introduced inside said encapsulation.

21. Shaping of at least one microparticle according to claim 18, wherein the microparticle has a body at least partially assembled from small particles formed of a first composition and are at least partially solidified and wherein second small particles formed of at least one of the first composition and a different composition are introduced into said reaction space and are at least partially assembled thereby encapsulating the body and forming at least two layers.

22. Shaping of at least one microparticle according to claim 1, wherein the microparticle has an outer surface that is only partly solidified.

23. Shaping of at least one microparticle according to claim 1, wherein a quantity of material, comprising one of a small particle, a dispersed phase and an aggregation of small particles, is added to said reaction space defined by front areas of said electrodes, and said electrodes are driven such that said body shaped in said reaction space protrudes from said reaction space by extending beyond tips of the electrodes.

24. Shaping of at least one microparticle according to claim 1, wherein the forces due to the varying electrical voltages driving the spatially disposed electrodes form a field in the reaction space and wherein the microparticle has a body that is only partially solidified inside the field, the microparticle being removable from said reaction space by one of elastic and plastic deformation, and is subjected to further solidification outside said reaction space.

25. Shaping of at least one microparticle according to claim 1, wherein the microparticle encapsulates a gas bubble.

26. Shaping of at least one microparticle according to claim 1, wherein

the surrounding liquid contains at least one of living cells, cells dividing and growing in the reaction space, immobilized cells, and dead cells that are assembled into a microparticle in the reaction space.

27. Shaping of at least one microparticle according to claim 1, wherein the small particles are at least one of viruses and macromolecules.

28. Shaping of at least one microparticle according to claim 1, wherein the microparticles are assembled into at least one of a disc and a disc with an indentation and are at least one of smaller than and as large as blood cells, thereby corresponding in geometry to the blood cells.

29. Shaping of at least one microparticle according to claim 1, wherein the at least two spatially disposed electrodes define at least one microchannel and the surrounding liquid is introduced into the reaction space and removed from the reaction space through the microchannel.

30. Shaping of at least one microparticle according to claim 1, wherein the microparticle is at least one of asymmetrical and perforated.

31. Shaping of at least one microparticle according to claim 1, wherein the microparticle is at least one of shaped, deformed and dielectrically broken at least one of during and after solidifying.

32. Shaping of at least one microparticle according to claim 1, wherein the surrounding liquid at least one of de-mixes and dissociates into different phases after introducing into the reaction space.

33. Shaping of at least one microparticle according to claim 32, wherein de-mixing is induced via said electrodes by at least one of ultrasonics or electric fields.

34. Shaping of at least one microparticle according to claim 1, wherein the microparticle has a body that is shaped to a predetermined mass by at least one of addition of material and removal of material by one of caustic and ablation.

35. Shaping of at least one microparticle according to claim 1, wherein the microparticle has a body that is shaped by a liquid stream.

36. Shaping of at least one microparticle according to claim 1, wherein the surrounding liquid is at least one of modified and exchanged during a time interval provided during assembly and solidification.

37. Shaping of at least one microparticle according to claim 1, comprising selecting a predetermined shape and driving the electrodes with controlled varying electrical voltages thereby consolidating the small particles to obtain said predetermined shape.

38. Shaping of at least one microparticle according to claim 1, wherein:

(a) the varying electrical voltages have an A.C. signal upon which a D.C. voltage component is superimposed; and

(b) at least one of the A.C. and D.C. signal components are intermittently switched off during assembly of the microparticle.

39. Shaping of at least one microparticle according to claim 1, wherein the microparticle is shaped by suspending the at least one small particle, dispersed phase and particle aggregate in the surrounding liquid.

40. Shaping of at least one microparticle according to claim 1, wherein the interspace between the electrodes is less that 10 μ

m.

41. Shaping of at least one microparticle according to claim 1, wherein the interspace between the electrodes is less that 1 μ

m.

42. Shaping of at least one microparticle according to claim 1, wherein one of the height and width of the electrodes is less that 10 μ

m.

43. Shaping of at least one microparticle according to claim 1, wherein one of the height and width of the electrodes is less that 1 μ

m.

44. Shaping of at least one microparticle according to claim 1, wherein the microparticle has a body at least partially assembled from living cells and wherein second small particles formed of a different composition are introduced into said reaction space and the body is at least partially assembled from the second small particles thereby encapsulating the body and forming at least two layers, said encapsulation being at least one of permeable or perforated.

45. A process for shaping at least one microparticle, comprising:

providing at least two spatially disposed electrodes thereby defining a reaction space fillable with a surrounding liquid, the electrodes having a width, a height and defining an interspace between the electrodes;

driving the electrodes with varying electrical voltages;

providing at least one of;

at least one small particle;

an aggregate of small particles;

a surrounding liquid with at least one small particle suspended therein;

a surrounding liquid with a dispersed phase; and

, a surrounding liquid with at least one small particle and a dispersed phase, at least one of a relative dielectric constant and a specific conductivity of the small particle and the dispersed phase, respectively, at a selected varying electrical voltage of said electrodes being lower than that of said surrounding liquid;

at least partially immersing the electrodes in the surrounding liquid thereby filling the reaction space with surrounding liquid;

wherein forces due to the varying electrical voltages driving the electrodes act on one of said at least one small particle and said dispersed phase, the varying electrical voltages driving the electrodes being adjusted such that at least one of said at least one small particle suspended in said surrounding liquid and said dispersed phase is formed as a differently shaped microparticle and at least partially permanently shaped by one of cross-linking, hardening and solidifying; and

,

wherein the forces due to the varying electrical voltages driving the spatially disposed electrodes form a field cage and a plurality of adjacent spatially disposed electrodes are provided defining a plurality of adjacent field cages and a plurality of microparticles are formed adjacent to each other in the plurality of adjacent field cages, the adjacent spatially disposed electrodes being arranged to form a channel as the reaction space and a microparticle in a form of one of a band and a chain of predeterminable length is formed in the plurality of adjacent field cages along the reaction space channel, the reaction space being defined by a plurality of electrode multipoles disposed along said reaction space, the multipoles being arranged in a plane perpendicular an extension of the elongated reaction space.

46. The process according to claim 45, wherein the electrode multipoles have at least two electrodes on at least two sides along the reaction chamber and wherein one of the width and the height of the electrodes is less than 1 μ

m.

47. The process according to claim 45, wherein each one of the electrode multipoles is a quadrupole.

48. The process according to claim 45, wherein more than two electrode multipoles are lined up in sequence along the extension of the elongated reaction space.