Device and method for DNA amplification and assay

• US 5,948,673 A
• Filed: 06/18/1997
• Issued: 09/07/1999
• Est. Priority Date: 09/12/1995
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Cottingham, Hugh V.
• Primary Examiner(s): Horlick, Kenneth R.
• Assistant Examiner(s): Siew, Jeffrey
• Application Number:
  US08/878,096
• Time in Patent Office: 811 Days
• Field of Search: 435/6, 435/91.1, 435/183, 435/287.2, 435/288.3, 435/305.1, 435/305.2, 435/305.3, 435/305.4, 422/30
• US Class Current: 435/287.2
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6445 Measuring fluorescence polarisation
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16

Abstract

A DNA amplification and homogeneous DNA probe assay device is provided which includes a multiplicity of discrete sample cells in a flat "card" format, with each sample cell containing the reagents necessary for both DNA amplification and homogeneous DNA probe assay. The device is particularly suitable for fluorescence polarization DNA probe assays, aid is preferably provided with and integal polarizer to avoid the need for polarizing elements in the related measuring apparatus. The size and geometry of the sample cells allows for a "hot start" of the DNA amplification reaction and thereby avoids mis-priming of the amplification reaction.

Claims

1. An apparatus for carrying out a homogeneous nucleic acid amplification and nucleic acid assay on a liquid biological sample, comprising:

a sample cell for receiving a liquid biological sample, said sample cell having a sample chamber in said sample cell;

a first opening which serves as a sample port for admitting said liquid biological sample into said sample chamber;

a second opening which serves as an air vent for allowing air to be displaced from said sample chamber during admission of said liquid biological sample into said sample cell; and

a dried nucleic acid amplification reagent and a dried homogeneous nucleic acid assay reagent, said reagents being adhered to the interior of said sample chamber for reacting with said liquid biological sample.

2. An apparatus as claimed in claim 1, wherein said dried nucleic acid amplification reagent and said homogeneous nucleic acid assay reagent are provided in the form of a single spot adhered to an internal surface of said sample chamber.

3. An apparatus as claimed in claim 1, further comprising a sealing member attachable to said sample cell for sealing said sample port after said liquid biological sample has been admitted to said sample chamber.

4. An apparatus as claimed in claim 1, wherein said homogeneous nucleic acid assay reagent comprises a fluorescence polarization assay reagent, and wherein at least a portion of said sample cell is sufficiently light-transmissive to permit external detection of a fluorescence polarization reaction occurring in a liquid biological sample contained in said sample chamber.

5. An apparatus as claimed in claim 3, wherein:

said homogeneous nucleic acid assay reagent comprises a fluorescence polarization assay reagent;

at least a portion of said sample cell is sufficiently light-transmissive to permit external detection of a fluorescence polarization reaction occurring in a liquid biological sample contained in said sample chamber; and

said sealing member is made of a light-transmissive material and is attachable over said light-transmissive portion of said sample chamber.

6. An apparatus as claimed in claim 5, wherein at least one of said sealing member and said light-transmissive portion of said sample chamber is made of a light-polarizing material.

9. A method for carrying out an integrated nucleic acid amplification and homogeneous nucleic acid fluorescence detection reaction on a liquid biological sample comprising:

providing the apparatus of claim 1;

preheating the sample cell of said apparatus to a temperature suitable for nucleic acid amplification;

introducing a liquid biological sample into said preheated sample cell to bring said liquid biological sample into contact with said dried nucleic acid amplification reagent and said dried homogeneous nucleic acid fluorescence detection assay reagent;

equilibrating the temperature of said liquid biological sample to the temperature of said preheated sample cell; and

after said temperature equilibration is complete, commencing said nucleic acid amplification reaction in said sample cell.

10. A method as claimed in claim 9, wherein the step of equilibrating the temperature of said liquid biological sample to the temperature of said preheated sample cell comprises forming a thin layer of said liquid biological sample in said sample cell to enhance heat transfer between said sample cell and said liquid biological sample.

11. A method as claimed in claim 9, wherein the time required for said temperature equilibration to occur is substantially the same as the time required for said liquid biological sample to dissolve said dried nucleic acid amplification reagent.

7. An apparatus as claimed in 1, wherein:

said sample cell is formed in a laminated card-like member having a bottom layer forming a bottom wall of said sample chamber, an apertured middle layer forming side walls of said sample chamber, and a top layer forming an upper wall of said sample chamber, said sample port being formed in said top layer; and

said sample cell is one of a plurality of sample cells disposed in a two-dimensional array across the length and width of said card-like member.

8. An apparatus as claimed in claim 7, further comprising a sealing member for sealing the sample ports of said sample cells after liquid biological samples have been admitted to said sample chamber, said sealing member comprising a layer of flexible material which is attachable to the top layer of said laminate by means of a pressure-sensitive adhesive carried on the underside of said layer of flexible material.