Process of bio-conversion of industrial or agricultural cellulose containing organic wastes into a proteinaceous nutrition product

• US 5,952,020 A
• Filed: 02/02/1999
• Issued: 09/14/1999
• Est. Priority Date: 09/10/1998
• Status: Expired due to Fees

• Current Assignee: Bio-FD & C Co., Ltd.
• Original Assignee: Bio-FD & C Co., Ltd.
• Inventors: Lizak, Yuri
• Primary Examiner(s): Lilling, Herbert J.
• Application Number:
  US09/241,430
• Time in Patent Office: 224 Days
• Field of Search: 435/42, 435/71.1, 426/53, 426/8, 426/13, 426/52, 426/54, 426/56
• US Class Current: 426/8
• CPC Class Codes: A23K 10/12 by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
• CPC Class Codes: A23K 10/32 from hydrolysates of wood or straw
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 21/00 Preparation of peptides or proteins single cell protein C12N1/00
• CPC Class Codes: C12P 39/00 Processes involving microorganisms of different genera in the same process, simultaneously

Abstract

A process of conversion of low protein, cellulose containing waste into a fodder or fodder supplement is provided. The process is effected by (a) inoculating the waste under aerobic conditions with a first inoculum including at least one first microorganism capable of converting cellulose into carbohydrates and at least one second microorganism capable of converting carbohydrates into proteins to thereby efficiently convert at least a portion of the cellulose into nutritional proteins and prevent accumulation of the carbohydrates and thereby inhibition of the conversion of the cellulose into the carbohydrates; and (b) inoculating the waste, under anaerobic conditions, with a second inoculum including at least one third microorganism capable of converting cellulose into carbohydrates and at least one fourth microorganism capable of converting carbohydrates into a preservative organic acid to thereby efficiently convert at least a portion of the cellulose into the preservative organic acid and prevent accumulation of carbohydrates and thereby inhibition of the conversion of the cellulose into the carbohydrates.

Claims

1. A process of conversion of low protein, cellulose containing waste into a fodder or fodder supplement, the process comprising the steps of:

(a) inoculating the waste under aerobic conditions with a first inoculum including at least one first microorganism capable of converting cellulose into carbohydrates and at least one second microorganism capable of converting carbohydrates into proteins to thereby efficiently convert at least a portion of said cellulose into nutritional proteins and prevent accumulation of said carbohydrates and thereby inhibition of said conversion of said cellulose into said carbohydrates; and

(b) inoculating the waste, under anaerobic conditions, with a second inoculum including at least one third microorganism capable of converting cellulose into carbohydrates and at least one fourth microorganism capable of converting carbohydrates into a preservative organic acid to thereby efficiently convert at least a portion of said cellulose into said preservative organic acid and prevent accumulation of carbohydrates and thereby inhibition of said conversion of said cellulose into said carbohydrates.

2. The process of claim 1, wherein said anaerobic conditions are effected by packaging the cellulose containing waste within a vacuum treated airtight bag.

3. The process of claim 1, wherein said first inoculum and said second inoculum are added concomitantly to the cellulose containing waste.

4. The process of claim 1, wherein said at least one first microorganism and said at least one second microorganism of said first inoculum are a single microorganism capable of converting cellulose into carbohydrates and converting carbohydrates into proteins.

5. The process of claim 4, wherein said single microorganism is a genetically modified yeast capable of secreting cellulose degrading enzymes.

6. The process of claim 5, wherein said yeast is a stably transformed Saccharomyces cerevisiae strain expressing cellodextrinase, endo-beta-1,4-glucanase, cellobiase and cellobiohydrolase.

7. The process of claim 1, further comprising the addition to said waste of at least one substance suitable for the nutrition of said first inoculum and said second inoculum.

8. The process of claim 7, wherein said substance is an organic or inorganic substance selected from the group consisting of (NH₄)₂ SO₄, urea, NaCl and animal or poultry manure.

9. The process of claim 1, wherein said at least one first microorganism is an edible microorganism, said microorganism being a fungus or bacteria of a genus selected from the group consisting of Humicola, Trichoderma, Penicillium, Ruminococcus, Bacillus, Cytophaga and Sporocytophaga.

10. The process of claim 9, wherein said at least one first microorganism is selected from the group consisting of Humicola grisea, Trichoderma harzianum, Trichoderma lignorum, Trichoderma reesei, Penicillium verruculosum, Ruminococcus albus, Bacillus subtilis, Bacillus thermoglucosidasius, Cytophaga spp. and Sporocytophaga spp.

11. The process of claim 1, wherein said at least one second microorganism is an edible microorganism, said microorganism being a fungus of a genus selected from the group consisting of Saccharomyces, Candida and Debaromyces.

12. The process of claim 11, wherein said at least one second microorganism is selected from the group consisting of Saccharomyces cerevisiae, Candida blankii and Debaromyces hansenii.

13. The process of claim 1, wherein said at least one third microorganism is an edible microorganism, said microorganism being a bacteria of a genus Ruminococcus.

14. The process of claim 13, wherein said at least one third microorganism is selected from the group consisting of Ruminococcus albus and Ruminococcus flavefaciens.

15. The process of claim 1, wherein said at least one fourth microorganism is an edible microorganism, said microorganism being a bacteria of a genus Lactococcus.

16. The process of claim 15, wherein said at least one fourth microorganism is selected from the group consisting of Lactococcus lactis subsp lactis, Lactococcus lactis subsp cremoris, Lactococcus plantarum, Lactococcus lactis subsp. hordniae and Lactococcus lactis subsp. cremoris.

17. The process of claim 1, wherein said waste is mechanically degraded into a particulate form.

18. The process of claim 1, wherein said waste is moistened by water to 45-65% of the dry weight of said waste.

19. The process of claim 1, wherein said cellulose containing waste is selected from the group consisting of plant waste, paper waste and animal manure waste.

20. The process of claim 1, wherein said aerobic step prolongs at least 10 hours.

21. The process of claim 1, wherein said aerobic step prolongs 10-72 hours.

22. The process of claim 1, wherein said anaerobic step prolongs at least 1 day.

36. A highly nutritious fodder or fodder supplement produced from a low protein, high cellulose containing waste material, the fodder or fodder supplement as produced by the process of claim 1 comprising:

(a) 100-400% more protein content, by weight, as compared with the low protein, high cellulose containing waste material.

37. The highly nutritious fodder or fodder supplement of claim 36, further comprising:

(b) 10-40% less cellulose content, by weight, as compared with the low protein, high cellulose containing waste material.

38. The highly nutritious fodder or fodder supplement of claim 37, wherein said fodder or fodder supplement includes 7-35% by weight protein and 5-15% by weight lactic acid.

39. The highly nutritious fodder or fodder supplement of claim 37, further comprising:

(c) 20-250% more fat content, by weight, as compared with the low protein, high cellulose containing waste material.

40. The highly nutritious fodder or fodder supplement of claim 39, wherein said fodder or fodder supplement includes 7-35% by weight protein, 0.5-6.5% by weight fat and 5-15% by weight lactic acid.

23. A starting culture for conversion of low protein, cellulose containing, waste into a high protein fodder or fodder supplement, the starting culture comprising:

(a) at least one first microorganism capable of converting cellulose into carbohydrates under aerobic conditions;

(b) at least one second microorganism capable of converting carbohydrates into proteins under aerobic conditions;

(c) at least one third microorganism capable of converting cellulose into carbohydrates under anaerobic conditions; and

(d) at least one fourth microorganism capable of converting carbohydrates into a preservative organic acid under anaerobic conditions.

24. The starting culture of claim 23, wherein said at least one first microorganism is an edible microorganism, said microorganism being a fungus or bacteria of a genus selected from the group consisting of Humicola, Trichoderma, Penicillium, Ruminococcus, Bacillus, Cytophaga and Sporocytophaga.

25. The starting culture of claim 24, wherein said at least one first microorganism is selected from the group consisting of Humicola grisea, Trichoderma harzianum, Trichoderma lignorum, Trichoderma reesei, Penicillium verruculosum, Ruminococcus albus, Bacillus subtilis, Bacillus thermoglucosidasius, Cytophaga spp. and Sporocytophaga spp.

26. The starting culture of claim 23, wherein said at least one second microorganism is an edible microorganism, said microorganism being a fungus of a genus selected from the group consisting of Saccharomyces, Candida and Debaromyces.

27. The starting culture of claim 26, wherein said at least one second microorganism is selected from the group consisting of Saccharomyces cerevisiae, Candida blankii and Debaromyces hansenii.

28. The starting culture of claim 23, wherein said at least one third microorganism is an edible microorganism, said microorganism being a bacteria of a genus Ruminococcus.

29. The starting culture of claim 28, wherein said at least one third microorganism is selected from the group consisting of Ruminococcus albus and Ruminococcus flavefaciens.

30. The starting culture of claim 23, wherein said at least one fourth microorganism is an edible microorganism, said microorganism being a bacteria of a genus Lactococcus.

31. The starting culture of claim 30, wherein said at least one fourth microorganism is selected from the group consisting of Lactococcus lactis subsp lactis, Lactococcus lactis subsp cremoris, Lactococcus plantarum, Lactococcus lactis subsp. hordniae and Lactococcus lactis subsp. cremoris.

32. The starting culture of claim 23, wherein said at least one first microorganism and said at least one second microorganism are a single microorganism capable of converting cellulose into carbohydrates and converting carbohydrates into proteins.

33. The starting culture of claim 32, wherein said single microorganism is a genetically modified yeast capable of secreting cellulose degrading enzymes.

34. The starting culture of claim 33, wherein said yeast is a stably transformed Saccharomyces cerevisiae strain expressing cellodextrinase, endo-beta-1,4-glucanase, cellobiase and cellobiohydrolase.

35. The starting culture of claim 23, wherein said first, second, third and fourth microorganisms are each independently provided in a form selected from the group consisting of dry powder of freeze dried viable cells, spores, frozen glycerol stock of viable cells, liquid culture, dried liquid culture and stab culture.