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Sets of labeled energy transfer fluorescent primers and their use in multi component analysis

  • US 5,952,180 A
  • Filed: 05/19/1998
  • Issued: 09/14/1999
  • Est. Priority Date: 01/15/1997
  • Status: Expired due to Fees
First Claim
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1. A method of sequencing a DNA molecule, said method comprising:

  • enzymatically producing four sets of primer extension products using said DNA molecule as a template, wherein each of said sets comprises a family of different sized primer extension products terminating at the same base, and further wherein each of said sets comprises the same fluorescent label, wherein the fluorescent labels from at least two of said sets comprise the same donor and acceptor fluorophores in energy transfer relationship separated by different distances and capable of providing distinguishable fluorescence emission patterns,size separating said primer extension products;

    detecting said size separated primer extension products; and

    determining said sequence from said detected size separated primer extension products.

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