Process for processing sucrose into glucose and fructose

• US 5,952,205 A
• Filed: 02/06/1998
• Issued: 09/14/1999
• Est. Priority Date: 02/06/1998
• Status: Expired due to Fees

• Current Assignee: Magnolia Nutritionals LLC
• Original Assignee: Neose Technologies, Inc.
• Inventors: Catani, Steven J., Navia, Juan L., Roth, Stephen A., McGuire, Edward J.
• Primary Examiner(s): Lilling, Herbert J.
• Application Number:
  US09/019,709
• Time in Patent Office: 585 Days
• Field of Search: 435/101, 435/105, 435/193
• US Class Current: 435/101
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)
• CPC Class Codes: C12P 19/24 produced by the action of an isomerase, e.g. fructose

Abstract

The present invention relates to a process of preparing commercial quantities of glucose and/or fructose from sucrose, a process of preparing commercial quantities of glucose and a polyfructan from sucrose, a reactor for practicing same, a process of preparing commercial quantities of fructose and a polyglucan from sucrose and a reactor for practicing same.

Claims

1. A process for preparing glucose from sucrose comprising:

i) contacting sucrose with a fructosyltransferase to produce glucose; and

ii) isolating glucose therefrom

wherein said reaction product further comprises a polyfructan and said process further comprises isolating said polyfructan.

2. The process of claim 1, wherein said fructosyltransfease is obtained from an organism selected from the group consisting of Aureobasidium pullulans, Aspergillus oryzae, Aspergillus awamori, Aspergillus sydowi, Aureobasidium sp., Aspergillus niger, Penicillium roquefortii, Streptococcus mutans, Penicillium jancezewskii and higher plants.

3. The process of claim 1, wherein said frucotosyltransferase is obtained by expression of a non-native fructosyltransferase gene.

4. The process of claim 1, wherein said glucose is isolated continuously or semibatchwise.

5. The process of claim 1, wherein said polyfructan is continuously isolated.

6. The process of claim 1, wherein said glucose is isolated by membrane filtration or chromatography.

7. The process of claim 1, wherein said polyfructan is isolated by membrane filtration or chromatography.

8. A process for preparing commercial quantities of glucose and a polyfructan of comprising:

i) contacting sucrose with a fructosyltransferase to produce a reaction product comprising glucose and a polyfructan; and

ii) isolating glucose and said polyfructan.

9. The process of claim 8, wherein said polyfructan is DP_5-6.

10. The process of claim 9, wherein said glucose is isolated continuously.

11. The process of claim 10, wherein said glucose is isolated continuously by membrane filtration or chromatography.

12. A process of preparing glucose and fructose from sucrose comprising:

i) contacting sucrose with a fructosyltransferase to produce glucose;

ii) contacting sucrose with a glucosyltransferase to produce fructose;

iii) isolating glucose and fructose.