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Method of assaying specimen substance by controlling dose of chemiluminescence

  • US 5,952,238 A
  • Filed: 10/08/1997
  • Issued: 09/14/1999
  • Est. Priority Date: 02/02/1995
  • Status: Expired due to Term
First Claim
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1. A method for assay of an analyte which comprises the steps of:

  • (1) providing a first probe that specifically binds to said analyte wherein said first probe is labeled with a chemiluminescent substance;

    (2) adding an unlabeled, second probe that specifically binds to said analyte;

    (3) contacting said first labeled probe and said unlabeled second probe with said analyte;

    (4) quantitatively measuring a chemiluminescent signal, wherein said unlabeled second probe decreases the chemiluminescent signal by decreasing a specific activity of the chemiluminescent substance of said first labeled probe;

    (5) adding a quencher to said analyte before said measuring step to decrease said chemiluminescent signal and decrease background noise levels of said chemiluminescent signal; and

    (6) correlating said chemiluminescent signal to the presence of said analyte.

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