Method of determining an alkali ion

• US 5,952,491 A
• Filed: 05/27/1998
• Issued: 09/14/1999
• Est. Priority Date: 05/30/1997
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics Corporation (Roche Holding AG)
• Original Assignee: AVL Medical Instruments
• Inventors: He, Huarui, Leiner, Marco Jean Pierre, Boila-Gockel, Andrei
• Primary Examiner(s): Raymond, Richard L.
• Assistant Examiner(s): Sripada, Pavanaram K
• Application Number:
  US09/085,218
• Time in Patent Office: 475 Days
• Field of Search: 540/467, 540/450, 436/74
• US Class Current: 540/467
• CPC Class Codes: C07D 413/10 linked by a carbon chain containing aromatic rings
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence

Abstract

The invention relates to a method of determining an alkali ion in a sample, wherein the alkali ion is contacted with a compound having a luminophoric moiety and an ionophoric moiety, which ionophoric moiety reacts with the alkali ion present in the sample, wherein the luminophoric moiety changes its luminescence properties, after which the luminescence is measured and the alkali ion determined utilizing the test reading,

which method is characterized in that the compound utilized is a monoaza-crown ether of the general Formula I ##STR1## in which X is the luminophoric moiety, m is the number 0, 1 or 2, and r and s independently mean the numbers 0, 1 or 2.

Claims

1. Method of determining an alkali ion in a sample comprising the steps of:

providing a compound having a luminophoric moiety and an ionophoric moiety

reacting the ionophoric moiety with the alkali ion present in the sample, wherein the luminophoric moiety changes its luminescence properties,

measuring the luminescence and

determining the presence of the alkali ion in the sample utilizing the measured luminescence, wherein the compound is a monoaza-crown ether of the general Formula I ##STR6## in which x is the luminophoric moiety, m is one selected from the group consisting of 0, 1 or 2, and r and s independently are selected from the group consisting of 0, 1 or 2.

2. Method according to claim 1 wherein r is 1 and s is 0.

3. Method according to claim 1 wherein r is 2 and s is 1.

4. Method according to any one of claims 1, 2 or 3 wherein x is selected from the group consisting of an amino-naphthalimide group and a xanthenone group wherein the amino-naphthalimide group has the general Formula II ##STR7## wherein at least one of R₁, R₂, R₃, R₄, R₅ and R₆ is an --NH-- group through which x is bound to the group --(CH₂)_m -- and the remaining groups and R₇ independently are selected from the group consisting of hydrogen, a lipophilic group, a hydrophilic group and a reactive group for coupling to a polymer and

the xanthenone group has the general Formula III ##STR8## wherein m is o and at least one of R₈, R₉, R₁₀, R₁₁, R₁₂, R₁₃, R₁₄ and R₁₅ represents a chemical bond through which x is bound directly to the ionophoric moiety and the remaining groups are each selected from the group consisting of --OH, OR₁₆ wherein R₁₆ is a hydrophilic or a lipophilic group, --O--R₁₇ --G wherein R₁₇ is a hydrophilic or a lipophilic group and G is a reactive group for coupling to a polymer, and --(CH₂)n--COOH wherein n is a number between 0 and 17.

6. Monoaza-crown ether in accordance with claim 5 wherein x is a luminophoric moiety as set forth in claim 4.

5. Monoaza-crown ether of the general Formula I ##STR9## wherein x is a luminophoric moiety and m is selected from the group consisting of 0, 1, and 2, and r and s independently are selected from the group consisting of 0, 1 and 2.