Multilayered submucosal graft constructs and method for making the same
First Claim
1. A method of preparing a multi-laminate tissue graft construct having pseudoisotropic properties, said construct formed from at least three strips of intestinal submucosal tissue delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of a wanm blooded vertebrate, each strip of intestinal submucosal tissue having a longitudinal axis corresponding to the predominant orientation of the collagen fibers in the submucosal tissue strips, said method comprising the steps ofoverlaying said first strip with at least two additional strips of submucosal tissue so that the longitudinal axis of each individual strip of submucosal tissue forms an angle of about 180°
- /N with the longitudinal axes of two other strips of overlaid submucosal tissue, wherein N=the total number of strips of submucosal tissue;
compressing at least the overlapped portions of the strips of submucosal tissue between two surfaces under conditions allowing at least partial dehydration of the compressed submucosal tissue.
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Accused Products
Abstract
Multi-laminar tissue graft constructs, pseudoisotropic graft constructs and a method of preparing such constructs are described. Multi-laminar tissue graft constructs are prepared by fusing partially overlapped strips of submucosa tissue into a unitary graft construct by compressing the tissue under dehydrating conditions. Pseudoisotropic tissue graft constructs are prepared by overlapping a first strip of submucosa tissue with at least two strips of submucosa tissue orientated so that the longitudinal axis of each strip formts an angle of about 180°/N with the longitudinal axes of at least two other strips of submucosal tissue.
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Citations
26 Claims
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1. A method of preparing a multi-laminate tissue graft construct having pseudoisotropic properties, said construct formed from at least three strips of intestinal submucosal tissue delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of a wanm blooded vertebrate, each strip of intestinal submucosal tissue having a longitudinal axis corresponding to the predominant orientation of the collagen fibers in the submucosal tissue strips, said method comprising the steps of
overlaying said first strip with at least two additional strips of submucosal tissue so that the longitudinal axis of each individual strip of submucosal tissue forms an angle of about 180° - /N with the longitudinal axes of two other strips of overlaid submucosal tissue, wherein N=the total number of strips of submucosal tissue;
compressing at least the overlapped portions of the strips of submucosal tissue between two surfaces under conditions allowing at least partial dehydration of the compressed submucosal tissue. - View Dependent Claims (2, 3, 4, 5, 6)
- /N with the longitudinal axes of two other strips of overlaid submucosal tissue, wherein N=the total number of strips of submucosal tissue;
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7. A unitary pseudoisotropic multi-laminate tissue graft construct, comprising at least three strips of intestinal submucosal tissue delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of a warm blooded vertebrate, wherein the strips are fused to one another by compressing the strips under dehydrating conditions, each strip having a longitudinal axis corresponding to the predominant orientation of the collagen fibers in the strips, wherein the longitudinal axis of each strip forms an angle of about 180°
- /N with the longitudinal axes of two other strips forming said construct, wherein N=the total number of strips of submucosal tissue.
- View Dependent Claims (8, 9, 18)
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10. A method of preparing a multi-laminate tissue graft construct from multiple strips of intestinal submucosal tissue delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of a warm blooded vertebrate, said strips having an abluminal surface and a luminal surface, said method comprising the steps of
placing a first strip of submucosal tissue on a support, so the luminal surface of the strip of submucosal tissue is in contact with the support; -
overlaying said first strip with additional strips of submucosal tissue wherein the last strip of submucosal tissue layered onto the tissue graft construct is orientated so the abluminal surface is in contact with the other layers of submucosal tissue forming the tissue graft construct; compressing the overlapped portions of the strips of submucosal tissue between two surfaces under conditions allowing at least partial dehydration of the compressed submucosal tissue. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 19, 23)
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20. A method of preparing a multi-laminate tissue graft construct from multiple strips of intestinal submucosal tissue delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of a warm blooded vertebrate, said strips having an abluminal surface and a luminal surface, said method comprising the steps of
placing a first strip of submucosal tissue on a support, so the luminal surface of the strip of submucosal tissue is in contact with the support; -
overlaying said first strip with a second strip of submucosal tissue to form a multi-layered construct, said second strip orientated so that the luminal surface of the second strip contacts at least a portion of the abluminal surface of the first strip; overlaying an additional strip of submucosal tissue onto said multi-layered construct, wherein said additional strips is overlaid with its abluminal surface in contact with at least a portion of abluminal surface of the multi-layered graft construct; compressing the overlapped portions of the strips of submucosal tissue between two surfaces under conditions allowing at least partial dehydration of the compressed submucosal tissue. - View Dependent Claims (21, 22, 24, 25, 26)
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Specification