Sequences for production of 2,4-Diacetylphloroglucinol and methods
First Claim
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1. A nucleic acid consisting of a Phl protein-encoding region linked to one or two heterologous nucleotide sequences, wherein the Phl protein is selected from a group consisting of a Pseudomonas fluorenscens PhlA, PhlB, PhlC, phlD, PhlE, PhlF, and PhlR.
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Abstract
DNA sequences which function specifically in the synthesis of 2,4-diacetylphloroglucinol (Phl) are described. The sequences include phl genes which encode phl gene proteins and coding and regulatory sequences for production of Phl as well as sequences containing phl genes, which sequences have the capability of conferring or enhancing Phl biosynthetic capability in bacterial strains. The transformed strains are useful as biocontrol agents against fungal pathogens.
17 Citations
51 Claims
- 1. A nucleic acid consisting of a Phl protein-encoding region linked to one or two heterologous nucleotide sequences, wherein the Phl protein is selected from a group consisting of a Pseudomonas fluorenscens PhlA, PhlB, PhlC, phlD, PhlE, PhlF, and PhlR.
- 10. A nucleic acid consisting of a Pseudomonas fluorenscens phl gene selected from a group consisting of a phlA, phlB, phlC, phlD, phlE, phlF, and phlR, wherein the phl gene is linked to one or two heterologous nucleotide sequences.
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20. A nucleic acid consisting of a Pseudomonas fluorenscens phl gene locus selected from the group consisting of:
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(a) the SalI-EcoRI fragment of pPHL5122; (b) the SalI-BamHI fragment of pMON5120; and (c) a nucleotide sequence selected from the group consisting of SEQ ID NO;
1, 2, 3 and 4, wherein the phl locus is linked to one or two heterologous nucleotide sequences. - View Dependent Claims (48)
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21. A phl gene probe consisting of a nucleotide sequence selected from a group consisting of SEQ ID NOS:
- 1,2, 5, 7, 9, 11, 13, 15, 17 and 19, or a fragment thereof at least 18 nucleotides in length and which hybridizes to the complementary strand of the corresponding SEQ ID NO;
1, 2, 5, 9, 11, 13, 15, 17 or 19 under high stringency wash conditions. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
- 1,2, 5, 7, 9, 11, 13, 15, 17 and 19, or a fragment thereof at least 18 nucleotides in length and which hybridizes to the complementary strand of the corresponding SEQ ID NO;
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32. A phl gene probe consisting of a nucleotide sequence selected from a group consisting of SEQ ID NO:
- 3, or a fragment thereof of greater than 18 nucleotides in length and which hybridizes to the complementary strand of SEQ ID NO;
3 under high stringency wash conditions.
- 3, or a fragment thereof of greater than 18 nucleotides in length and which hybridizes to the complementary strand of SEQ ID NO;
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33. A phl gene probe consisting of a nucleotide sequence selected from a group consisting of SEQ ID NO:
- 4, or a fragment thereof of greater than 18 nucleotides in length and which hybridizes to the complementary strand of SEQ ID NO;
4 under high stringency wash conditions.
- 4, or a fragment thereof of greater than 18 nucleotides in length and which hybridizes to the complementary strand of SEQ ID NO;
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49. A phl gene probe consisting of a nucleotide sequence selected from a group consisting of SEQ ID NOS:
- 1, 2, 5, 7, 9, 11, 13, 15, 17 and 19, or a fragment thereof of at least 18 nucleotides in length and which hybridizes to the complementary strand of the corresponding SEQ ID NO;
1, 2, 5, 7, 9, 11, 13, 15, 17 or 19 under low stringency wash conditions.
- 1, 2, 5, 7, 9, 11, 13, 15, 17 and 19, or a fragment thereof of at least 18 nucleotides in length and which hybridizes to the complementary strand of the corresponding SEQ ID NO;
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50. A phl gene probe consisting of a nucleotide sequence selected from a group consisting of SEQ ID NO:
- 3, or a fragment thereof of greater than 18 nucleotides in length and which hybridizes to the complementary strand of SEQ ID NO;
3 under low stringency wash conditions.
- 3, or a fragment thereof of greater than 18 nucleotides in length and which hybridizes to the complementary strand of SEQ ID NO;
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51. A phl gene probe consisting of a nucleotide sequence selected from a group consisting of SEQ ID NO:
- 4, or a fragment thereof of greater than 18 nucleotides in length and which hybridizes to the complementary strand of SEQ ID NO;
4 under low stringency wash conditions.
- 4, or a fragment thereof of greater than 18 nucleotides in length and which hybridizes to the complementary strand of SEQ ID NO;
Specification
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Current AssigneeUnited States Department Of Agriculture As Represented By The Secretary Of Agriculture, WSU Research Foundation
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Original AssigneeUnited States Department Of Agriculture, Washington State University
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InventorsThomashow, Linda S., Bangera, Mahalaxmi, Weller, David M., Cook, R. James
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Primary Examiner(s)Lau, Kawai
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Application NumberUS08/494,907Time in Patent Office1,548 DaysField of Search435/69.1, 435/252.3, 435/320.1, 435/183, 435/195, 530/350, 536/22.1, 536/23.7, 536/23.2, 536/24.32US Class Current435/69.1CPC Class CodesC07K 14/21 from Pseudomonadaceae (F)C12N 15/52 Genes encoding for enzymes ...
