Stable macroscopic membranes formed by self-assembly of amphiphilic peptides and uses therefor

• US 5,955,343 A
• Filed: 08/22/1994
• Issued: 09/21/1999
• Est. Priority Date: 12/28/1992
• Status: Expired due to Term

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Holmes, Todd, Zhang, Shuguang, Rich, Alexander, DiPersio, C. Michael, Lockshin, Curtis
• Primary Examiner(s): Patterson, Jr., Charles L.
• Assistant Examiner(s): GRIMES, ERIC BURTON
• Application Number:
  US08/293,284
• Time in Patent Office: 1,856 Days
• Field of Search: 435/240.1, 435/240.2, 435/240.23, 435/240.241
• US Class Current: 435/325
• CPC Class Codes: A61K 9/009 Sachets, pouches characterised by the material or function of the envelope with gastric retention A61K9/0065; sachets which are not administered but function merely as a container are classified according to the content, e.g. sachets comprising powder for reconstitution of a drink A61K9/0095
• CPC Class Codes: C07K 14/001 by chemical synthesis
• CPC Class Codes: C07K 14/395 from Saccharomyces
• CPC Class Codes: C07K 5/0815 with the first amino acid being basic
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids
• CPC Class Codes: C07K 7/08 having 12 to 20 amino acids gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 2533/50 Proteins
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

Described herein is the self-assembly of amphiphilic peptides, i.e., peptides with alternating hydrophobic and hydrophilic residues, into macroscopic membranes. The membrane-forming peptides are greater than 12 amino acids in length, and preferably at least 16 amino acids, are complementary and are structurally compatible. Specifically, two peptides, (AEAEAKAK)₂ (ARARADAD)₂, were shown to self-assemble into macroscopic membranes. Conditions under which the peptides self-assemble into macroscopic membranes and methods for producing the membranes are also described. The macroscopic membranes have several interesting properties: they are stable in aqueous solution, serum, and ethanol, are highly resistant to heat, alkaline and acidic pH, chemical denaturants, and proteolytic digestion, and are non-cytotoxic. The membranes are potentially useful in biomaterial applications such as slow-diffusion drug delivery systems, artificial skin, and separation matrices, and as experimental models for Alzheimer'"'"'s disease and scrapie infection. The sequence of the peptide, EAK16, was derived from a putative Z-DNA binding protein from yeast, called zuotin. The cloning and characterization of the ZUO1 gene are also described.

Claims

1. A method for in vitro cell culture comprising:

a) adding a macroscopic membrane which in formed by self-assembly of amphiphilic peptides in an aqueous solution containing monovalent metal cations, wherein the peptides are 12 or more amino acids in length, have alternating hydrophobic and hydrophilic amino acids, and are complementary and structurally compatible, to a call culture medium comprising cells, thereby forming a membrane/culture mixture;

b) maintaining the mixture under conditions sufficient for cell growth.

2. The method of claim 1 wherein the peptides are homogeneous.

3. The method of claim 1 wherein the macroscopic membrane was formed by self-assembly of a peptide (residues 310 to 317 of SEQ ID NO:

2) having the sequence (Ala-Glu-Ala-Glu-Ala-Lys-Ala-Lys)_n, where n is greater than or equal to 2.

4. The method of claim 1 wherein the macroscopic membrane was formed by a peptide (residues 1 to 8 of SEQ ID NO:

40) having the sequence (Arg-Ala-Asp-Ala-Arg-Ala-Asp-Ala)_n, where n is greater than or equal to 2.