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Self-contained device integrating nucleic acid extraction amplification and detection

  • US 5,955,351 A
  • Filed: 07/12/1996
  • Issued: 09/21/1999
  • Est. Priority Date: 07/13/1995
  • Status: Expired due to Term
First Claim
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1. A self-contained device for the extraction, amplification and detection of nucleic acid sequences, which comprises:

  • a) a first hollow elongated cylinder closed at one end having a plurality of chambers, each chamber having an upper end and a lower end;

    b) a second hollow elongated cylinder positioned contiguously inside said first cylinder and having an upper end and a lower end with an aperture and sealing lip interposed and connecting via rotation said lower end of the second cylinder to said upper end of each chamber of said first cylinder, said second cylinder further having three indexing notches disposed equilaterally on the upper end of the cylinder; and

    c) a cover integrally hinged to the open end of the first cylinder, said cover having a reaction bead chamber integral with a knife-edge, said chamber housing a reaction bead and hermetically sealed with a membrane, said cover further having an indexing pin disposed diametrically to the hinge for indexing with said notches during rotation of said first cylinder in relation to said second cylinder.

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