Instruments for chemical and microbiological tests

• US 5,955,352 A
• Filed: 06/20/1997
• Issued: 09/21/1999
• Est. Priority Date: 12/22/1994
• Status: Expired due to Term

• Current Assignee: Showa Yakuhin Kako Co., Ltd. (General Cannabis Corp.)
• Original Assignee: Showa Yakuhin Kako Co., Ltd. (General Cannabis Corp.)
• Inventors: Yamada, Hiroshi, Kinoshita, Yuichi, Inoue, Yoshiharu, Shibuya, Mutsumi, Ohshina, Chizuko, Hayashi, Masaho, Oguchi, Kiyoshi
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US08/879,599
• Time in Patent Office: 823 Days
• Field of Search: 435/287.7, 435/288.4, 435/288.5, 435/305.2, 435/305.3, 422/56, 422/58, 422/102
• US Class Current: 435/287.7
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/5088 confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence

Abstract

An instrument for chemical and microbiological tests comprises a sample container for temporarily storing a liquid sample; a plurality of sample-holding portions formed on the bottom face of the sample container; and a liquid-absorbent body capable of coming in contact with the liquid sample so that the body can absorb the excess sample, when the liquid sample is introduced into the container, while retaining the sample to be held on the sample-holding portions. This instrument can be used in a chemical or microbiological test wherein a sample is used in a relatively small amount and, in particular, those requiring a large number of reactions and cultivation. Moreover, the instrument does not require the dispensation of a sample to each reaction system and does not cause any scatter of the sample in the surroundings.

Claims

1. An instrument for chemical or microbiological tests comprising;

a sample container for temporarily storing a liquid sample;

a plurality of sample-holding portions formed on the bottom face of the sample container in the form of hydrophilic portions said sample-holding portions being positioned at a level higher than that of the portion on the bottom face of the container other than the sample-holding portion; and

a liquid-absorbent body capable of coming in contact with the liquid sample so that the body can absorb the excess sample, when the liquid sample is introduced into the container, while retaining the sample to be held on the sample-holding portions, said liquid-absorbent body being provided such that it constitutes the lowest portion of the bottom face of the container except for the sample-holding portion.

2. The instrument as set forth in claim 1 wherein the sample-holding portions are provided as concave portions on the bottom face of the container.

3. The instrument as set forth in claim 1 wherein the liquid-absorbent body is provided so as to constitute a part or the whole of the bottom face, partly or wholly surrounding the sample-holding portions, of the sample container for accommodating a liquid sample.

4. The instrument as set forth in claim 1 wherein the sample-holding portions contain an agent for a chemical or microbiological test.

5. An instrument for chemical or microbiological tests comprising a sample container for at least temporarily storing a liquid sample;

a plurality of sample-holding portions which are arranged on a hydrophobic bottom face of the sample container and spaced apart from one another;

a liquid-absorbent body which is provided so as to absorb the liquid sample present at the periphery of the sample-holding portions when the liquid sample is introduced into the container; and

a bottom member arranged behind the sample container and capable of supporting the liquid-absorbent body, the bottom member forming a region for accommodating the liquid-absorbent body in cooperation with the sample container, the sample container being provided with a peripheral portion positioned below the sample-holding portion and a through-hole formed on the peripheral portion, and the through-hole permitting the fluid communication with the liquid-absorbent body-accommodating region arranged between the sample container and the bottom member.

6. The instrument of claim 5 wherein the outer peripheral edge of the sample-holding portion is positioned at a level higher than that of the peripheral portion thereof and the peripheral portion is formed from a funnel-like wall body which downward extends from the outer edge towards the hole.

7. The instrument of claim 6 wherein the bottom member is provided with a plurality of upward extending cylindrical standing-up walls and the top face of the standing-up wall has a shape substantially complementary to the lower face of the sample-holding portion.

8. An instrument for chemical or microbiological tests comprising a sample container for at least temporarily storing a liquid sample;

a plurality of sample-holding portions which are arranged on a hydrophobic bottom face of the sample container and spaced apart from one another;

a liquid-absorbent body which is provided so as to absorb the liquid sample present at the periphery of the sample-holding portions when the liquid sample is introduced into the container; and

a bottom member formed in the form of a container whose top is opened, a plurality of cylindrical standing-up walls which upward extend, each having a top face, are formed on the bottom wall of the bottom member, the top face of the standing-up wall constitutes the sample-holding portion;

a plurality of through-holes, through which the standing-up wall passes, are formed on the liquid-absorbent body and a plurality of openings capable of being fitted to the top portions of the standing-up walls are formed, on the bottom face of the container.

9. The instrument of claim 8 wherein the standing-up wall is formed in the form of a cylinder and a stepped portion is formed on the outer periphery of the standing-up wall, which is fitted with the inner peripheral edge of the opening on the bottom face to thus position the top edge of the standing-up wall and the bottom face on a substantially the same plane.

10. An instrument for chemical or microbiological tests comprising a sample container for at least temporarily storing a liquid sample;

a plurality of sample-holding portions which are arranged on a hydrophobic bottom face of the sample container and spaced apart from one another;

a liquid-absorbent body which is provided so as to absorb the liquid sample present at the periphery of the sample-holding portions when the liquid sample is introduced into the container; and

a bottom member arranged behind the sample container and capable of supporting the liquid-absorbent body, the bottom member forming a region for accommodating the liquid-absorbent body in cooperation with the sample container, the sample container being provided with a peripheral portion positioned below the sample-holding portion and a through-hole formed on the peripheral portion, and the through-hole permitting the fluid communication with the liquid-absorbent body-accommodating region arranged between the sample container and the bottom member;

said bottom member having a bottom wall, wherein upper projections which support the liquid-absorbent body are arranged on the bottom wall of the bottom member and the projections are positioned below the through-holes.