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Biosensor device and method

  • US 5,955,379 A
  • Filed: 04/24/1997
  • Issued: 09/21/1999
  • Est. Priority Date: 04/25/1996
  • Status: Expired due to Fees
First Claim
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1. A biosensor apparatus for detecting binding between an oligosaccharide ligand and ligand-specific binding receptor, comprisingan electrode including(i) a detection surface,(ii) formed on said detection surface, a close-packed and ordered monolayer comprising hydrocarbon chains anchored at their proximal ends to the detection surface, said chains being sufficiently close-packed and ordered to form an effective barrier to inhibit contact of a redox species, in an aqueous solution in contact with the monolayer, with said detection surface and thereby reduce measurably electron flow across the monolayer mediated by said redox species, and(iii) said oligosaccharide ligand attached to the distal ends of a portion of said chains, such that binding of said ligand-specific binding receptor to said oligosaccharide ligand perturbs the monolayer sufficiently to allow said redox species to contact said detection surface to donate or accept electrons from said surface and thereby increase measurably electron flow across the monolayer mediated by said redox species,a chamber for holding said aqueous solution of redox species in contact with said monolayer,means for introducing said receptor into said chamber, andcircuit means, comprising voltage generating means, for measuring electron flow mediated by said redox species across said monolayer, in response to binding occurring between said receptor and said oligosaccharide ligand.

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