Surface plasmon resonance-mass spectrometry

• US 5,955,729 A
• Filed: 09/06/1996
• Issued: 09/21/1999
• Est. Priority Date: 09/08/1995
• Status: Expired due to Term

• Current Assignee: GE Healthcare Bio-Sciences AB (GE Healthcare Technologies, Inc.)
• Original Assignee: Biacore AB (General Electric Company)
• Inventors: Nelson, Randall W., Sjolander, Stefan, Krone, Jennifer R., Granzow, Russell, Jansson, Osten
• Primary Examiner(s): ANDERSON, BRUCE C
• Application Number:
  US08/708,341
• Time in Patent Office: 1,110 Days
• Field of Search: 250/282, 250/288, 250/292, 250/423 P
• US Class Current: 250/282
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: H01J 49/0418 for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
• CPC Class Codes: H01J 49/164 Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI] sample holders H01J49/0418

Abstract

The invention provides surface plasmon resonance-mass spectroscopy for the rapid, sensitive and accurate investigation of molecular interactions coupled with the identification and quantification of the same. Methods of the invention include capturing an analyte present within a sample by an interactive surface layer on a real-time interaction analysis sensor, analyzing the analyte by surface plasmon resonance while the analyte is captured by the interactive surface layer, and identifying the captured analyte by desorbing/ionizing the analyte from the interactive surface layer while under vacuum within a mass spectrometer. Devices of the invention include a transparent material, a conductive material capable of supporting surface plasmon resonance affixed to the transparent material, an interactive surface affixed to the conductive material, and a means for exposing the interactive surface to the interior of a mass spectrometer without breaking the vacuum therein.

Claims

1. A method for performing surface plasmon resonance-mass spectrometry on a sample, comprising:

capturing an analyte present within the sample by an interactive surface layer of an interaction analysis sensor;

analyzing the analyte by surface plasmon resonance which monitors a consequential change of a reflected first light beam while the analyte is captured by the interactive surface layer of the interaction analysis sensor; and

mass analyzing the captured analyte by desorbing/ionizing the analyte from the interactive surface layer of the interaction analysis sensor with a second light beam while under vacuum within a mass spectrometer.

2. The method of claim 1 wherein the interaction analysis sensor is a sensor chip.

3. The method of claim 1 wherein the interaction analysis sensor is a fiber optic sensor.

7. The method of claims 1 or 4 wherein the interactive surface layer is a hydrogel.

8. The method of claim 7 wherein the hydrogel is a hydrogel of a polysaccharide.

9. The method of claim 8 wherein the polysaccharide is a carboxymethylated detran.

14. The method of claims 1 or 4 wherein the second light beam is laser light.

17. The method of claims 1 or 4 wherein, prior to desorbing/ionizing the analyte, it is contacted with a laser desorption/ionization matrix.

4. A method for analyzing and identifying an analyte within a sample, comprising:

capturing the analyte by contacting the analyte with an interactive surface layer affixed to a conductive material capable of supporting surface plasmon resonance, wherein the conductive material has a front surface affixed to the interactive surface layer and a back surface affixed to a transparent layer;

directing a first light beam through the transparent layer at varying angles of incidence or wavelengths such that the first light beam is reflected off the back surface of the conductive material and excites surface plasmons at the interface between the conductive layer and the interactive surface laser;

detecting the angle of incidence or the wavelength at which the intensity of the reflected first light beam has a minimum due to surface plasmon resonance to determine the angular or wavelength change caused by the analyte captured by the interactive surface layer; and

measuring the mass spectrum of the analyte by desorbing/ionizing the analyte from the interactive surface layer with a second light beam while under vacuum within a mass spectrometer.

5. The method of claim 4 wherein the interactive surface layer affixed to the conductive material and the transparent layer is in the form of a chip.

6. The method of claim 4 wherein the interactive surface layer affixed to the conductive material and the transparent layer is in the form of a fiber optic.

10. The method of claim 4 wherein the conductive material is a metal.

11. The method of claim 10 wherein the metal is gold.

12. The method of claim 4 wherein the transparent layer is a glass.

13. The method of claim 4 wherein the first light beam is polarized light.

15. The method of claim 4 wherein the second light beam is laser light directed at the interactive layer such that it passes through the interactive layer before striking the front surface of the conductive material.

16. The method of claim 4 wherein the second light beam is laser light directed at the interactive layer such that it strikes the back surface of the conductive material and passes through the conductive material and into the interactive layer.