Article for dispensing small volumes of liquid

• US 5,957,167 A
• Filed: 12/18/1997
• Issued: 09/28/1999
• Est. Priority Date: 12/18/1997
• Status: Expired due to Fees

• Current Assignee: Pharmacopeia LLC (Ligand Pharmaceuticals, Inc.)
• Original Assignee: Pharmacopeia, Inc.
• Inventors: Feygin, Ilya
• Primary Examiner(s): JACYNA, J CASIMER
• Application Number:
  US08/993,106
• Time in Patent Office: 649 Days
• Field of Search: 141/1, 141/31, 141/130, 141/284, 73/864.02, 73/864.72, 422/100
• US Class Current: 141/31
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00369 in multiple or parallel arrangements
• CPC Class Codes: B01L 2400/021 non contact spotting by inertia, i.e. abrupt deceleration of the nozzle or pin
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/0248 Prongs, quill pen type dispenser
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 2035/00237 Handling microquantities of analyte, e.g. microvalves, capillary networks
• CPC Class Codes: G01N 2035/1037 Using surface tension, e.g. pins or wires
• CPC Class Codes: G01N 35/1016 Control of the volume dispensed or introduced
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00

Abstract

A method for handling and dispensing small volumes of liquid, and apparatus for carrying out the method, are disclosed. A small volume of liquid, which is retained within a fluid-dispensing member, is dispensed therefrom by accelerating, and then abruptly decelerating, the member. The abrupt deceleration causes the retained liquid to discharge. The discharged liquid may be directed toward a receiver. A micro volume liquid dispenser for practicing the method includes a plurality of fluid-dispensing members configured for aspiring and retaining a small liquid volume via capillary action. The dispenser further includes an actuator for moving/accelerating the fluid-dispensing members and for stopping/abruptly decelerating the fluid-dispensing members.

Claims

1. An article for dispensing a small volume of liquid, comprising:

a fluid-dispensing member having two opposed surfaces in spaced relation to one another, the surfaces and space therebetween operable to generate a capillary effect;

an actuator operable to accelerate and then abruptly decelerate the fluid-dispensing member, said abrupt deceleration causing liquid held between the two opposed surfaces to discharge therefrom; and

a liquid director physically configured to substantially eliminate deviations in a path followed by said liquid discharged from the fluid-dispensing member.

2. The article of claim 1, wherein the fluid-dispensing member has a discontinuity in the spaced opposed surfaces and the space therebetween, said discontinuity operable to terminate the capillary effect.

3. The article of claim 2, wherein the discontinuity is a widening of the space between the opposed surfaces.

4. The article of claim 1, wherein the physical configuration of the liquid director is substantially needle-like, and wherein the liquid director is disposed within the space between the opposed surfaces and aligned along a centrally-located long axis of the fluid-dispensing member.

5. The article of claim 4, wherein the liquid director extends beyond opposed surfaces of the fluid-dispensing member along the direction of the long axis.

6. The article of claim 1, wherein the opposed surfaces are concave.

7. The article of claim 1, wherein the opposed surfaces are flat.

8. The article of claim 1, wherein the fluid-dispensing member further comprises an adjustment device operably connected to the two opposed surfaces, the adjustment device being operable to change the spacing between said two opposed surfaces.

9. The article of claim 8, wherein the adjustment device is a tightening screw.

10. The article of claim 1, wherein the actuator comprises a biasing member operable to store energy in a first position, wherein, when released from the first position, a portion of the stored energy is converted to kinetic energy thereby accelerating at least a part of the biasing member and also accelerating the fluid-dispensing member, which is in mechanical communication therewith.

11. The article of claim 10, wherein the biasing member is a spring.

12. The article of claim 1, wherein the actuator further comprises

a first and a second stop, wherein the first stop is substantially nonmovable and the second stop is mechanically linked to the fluid-dispensing member and thereby accelerated therewith, and, wherein,

the first stop is suitably positioned to engage the second stop after the second stop has moved a predetermined distance as a result of said acceleration, wherein,

the fluid-dispensing member is abruptly decelerated when the first and second stops engage.

13. The article of claim 12, wherein the first stop is positioned to engage the second stop before substantially all stored energy available for conversion into kinetic energy is so converted.

14. The article of claim 1, further comprising an array of ninety-six fluid-dispensing members, wherein the ninety-six fluid-dispensing members are arranged so that when liquid in one of the fluid-dispensing members is dispensed, it is receivable by a complementary one of ninety-six wells arranged in an array on a suitably-positioned ninety-six well microtiter plate.

15. The article of claim 1, further comprising a microprocessor in communication with a memory and a clock, the microprocessor operable to actuate the actuator according to a dosing schedule.

16. The article of claim 15, wherein the microprocessor is in communication with a controllable current source, the controllable current source is in electrical communication with the actuator, and the actuator is a solenoid, wherein,

the microprocessor is operable to send a signal to the controllable current source at a time dictated by the dosing schedule, which controllable current source is operable to apply a current to the solenoid, thereby actuating the solenoid and accelerating the fluid-dispensing member.

17. The article of claim 15, wherein the actuator is configured for one of either pneumatic, hydraulic or electrodynamic operation.

18. A micro volume liquid dispenser, comprising:

a fluid-dispensing member operable to generate a capillary effect;

a biasing element mechanically linked to the fluid-dispensing member, wherein said biasing element is operable to accelerate said fluid-dispensing member;

a first stop member depending from said fluid-dispensing member, wherein said first stop member is accelerated with said fluid-dispensing member,

a second stop member that is substantially nonmovable and is aligned to engage the first stop member after said first stop member has been accelerated, and is further aligned to prevent said fluid-dispensing member from contacting a liquid receiving surface; and

a liquid director physically configured to substantially eliminate deviations in a path followed by said liquid discharged from the fluid-dispensing member.

19. The micro volume liquid dispenser of claim 18, wherein said fluid dispensing member comprises two opposed surfaces in spaced relation to one another, wherein there is a discontinuity in the spaced opposed surfaces and the space therebetween, said discontinuity operable to terminate said capillary effect.

20. A method for discharging a small volume of liquid, comprising:

accelerating a liquid carrier operable to retain liquid via a capillary effect;

abruptly decelerating the liquid carrier while preventing it from contacting a surface of a receiver; and

directing said liquid along a liquid director that extends beyond said liquid carrier towards said receiver.

21. The method of claim 20, wherein the step of accelerating further comprises converting the stored energy within a biasing element to kinetic energy that is imparted to the liquid carrier.

22. An article for dispensing a small volume of liquid, comprising:

a fluid-dispensing member having two opposed surfaces in spaced relation to one another, the surfaces and space therebetween operable to generate a capillary effect;

an actuator operable to accelerate and then abruptly decelerate the fluid-dispensing member, said abrupt deceleration causing liquid held between the two opposed surfaces to discharge therefrom; and

a tightening screw operably connected to the two opposed surfaces, the tightening screw operable to change the space between said two opposed surfaces.

23. The article of claim 22, wherein said fluid-dispensing member has a liquid director physically configured to substantially eliminate deviations in a path followed by said liquid discharged from the fluid-dispensing member.

24. An article for dispensing a small volume of liquid, comprising:

a fluid-dispensing member operable to generate a capillary effect;

means for accelerating said fluid-dispensing member;

means for rapidly decelerating said fluid dispensing member;

means for preventing said fluid-dispensing member from contacting a receiver that receives liquid dispensed from said fluid-dispensing member upon said rapid deceleration; and

means for substantially eliminating deviations in a path followed by said liquid dispensed from said fluid-dispensing member.

25. The article of claim 24, wherein said fluid-dispensing member comprises

two spaced opposed surfaces.

26. The article of claim 25, further comprising:

means for adjusting a space between said two spaced opposed surfaces.