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Detection of purine and pyrimidine nucleotides and underivatized nucleic acids by sinusoidal voltammetry

  • US 5,958,215 A
  • Filed: 07/18/1997
  • Issued: 09/28/1999
  • Est. Priority Date: 09/18/1995
  • Status: Expired due to Term
First Claim
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1. A method for detecting nucleotides and nucleic acid polymers in a sample comprising the steps of:

  • contacting a surface of an electrocatalytic metal electrode with the sample dissolved in a high pH aqueous buffer;

    applying a sinusoidal voltage having an amplitude and a fundamental frequency to the electrode, the amplitude being sufficient to elicit a redox reaction of at least one form of nucleotides and nucleic acid polymers in contact with the electrode;

    measuring a current at the electrode, the current resulting from the elicited redox reaction;

    producing a frequency spectrum of the measured current; and

    detecting the nucleotides and nucleic acid polymers by reference to a harmonic of the frequency spectrum greater than the fundamental frequency.

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