Thin film sample support

• US 5,958,345 A
• Filed: 03/14/1997
• Issued: 09/28/1999
• Est. Priority Date: 03/14/1997
• Status: Expired due to Fees

• Current Assignee: Moxtex, Inc.
• Original Assignee: Moxtek Inc (Nippon Kayaku Company Limited)
• Inventors: Turner, D. Clark, Wilson, Alisa A., Robbins, Arthur A.
• Primary Examiner(s): Thornton, Krisanne
• Application Number:
  US08/818,168
• Time in Patent Office: 928 Days
• Field of Search: 422/102, 422/104, 422/98, 422/82.05, 435/301, 436/531, 436/535, 428/409, 428/543
• US Class Current: 422/561
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/5088 confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence
• CPC Class Codes: G01N 23/2204 Specimen supports therefor; Sample conveying means therefore
• CPC Class Codes: Y10T 428/31 Surface property or characteristic of web, sheet or block

Abstract

A sample support for holding for samples for use with an analysis instrument. The sample support is particularly beneficial for use with analysis instruments which rely on a beam of radiation or accelerated particles and a method for making the same is disclosed. The holder includes a frame with one or more orifices covered by a support surface, typically in the form of a thin polymer film. The film is divided into hydrophobic and hydrophilic portions to isolate precise positions where samples can be placed to intersect a probe beam during analysis.

Claims

1. A sample holder for retaining a liquid sample in a localized region and configured for subsequent analytical processing in a beam of electromagnetic radiation, the sample holder comprising:

a substantially planar sample support surface for providing a localized region for receiving a test sample and having a central portion and an exterior portion disposed to substantially surround the central portion, one of the central portion and the exterior portion having a hydrophilic surface, and the other of the central portion and the exterior portion having a hydrophobic surface, the central and exterior portions of the sample support surface being formed of a material which resists spectral contamination.

2. The sample holder of claim 1, wherein the sample support surface is configured to use as part of an analytical processing system utilizing particle beams.

3. The sample holder of claim 2, wherein the sample support surface is formed by a polymer film.

4. The sample holder of claim 1, wherein the support surface has a composition having atomic components, and wherein each atomic component of the support surface has an atomic number less than that of fluorine.

5. The sample holder of claim 1, wherein the holder further comprises:

a support frame defining at least one orifice for holding the sample support surface and maintaining the sample support surface in a generally planar orientation, the sample support surface being disposed on the frame with the central portion disposed in alignment with the orifice such that an x-ray beam may pass through the central portion without contacting the frame.

6. The sample holder of claim 1, wherein the sample support surface is formed of a polymer film and wherein the central portion of the film has a surface density of less than about 50 μ

g/cm².

7. The sample holder of claim 1, wherein the central portion is configured to be subjected to x-ray electromagnetic energy, while maintaining nominal scattering of x-rays.

8. The sample holder of claim 1, wherein the central portion has a diameter of 2 mm or less.

9. The sample holder of claim 1, wherein the support surface comprises a polymer film having a plurality of central portions, each central portion having a hydrophilic surface, and each central portion being substantially surrounded by a exterior portion having a hydrophobic surface.

10. The sample holder of claim 1, wherein the support surface comprises a polymer film having a plurality of central portions, each central portion having a hydrophobic surface, and each central portion being substantially surrounded by a exterior portion having a hydrophilic surface.

11. The sample holder of claim 1, wherein the support surface comprises a polymer film which is formed of at least one of the group consisting of polyvinyl formal, polyimide, polycarbonate, polypropylene, polyethylene, parylene and isotactic polypropylene.

12. The sample holder of claim 11, wherein the film is at least partially formed of polyimide.

13. The sample holder of claim 5, wherein the support frame is comprised of a composition (i) which provides structural rigidity adapted for applications involving exposure of the sample holder to an analytical beam,, and (ii) which has a sufficiently low vapor pressure to preclude contamination of a test environment.

14. The sample holder of claim 1, further comprising positioning means disposed on the sample support surface for alignment with respect to an analytical beam.

15. The sample holder of claim 14, wherein the positioning means comprises a material that luminesces in an irradiating beam disposed on the central portion of the support surface.

16. The sample holder of claim 3, wherein the polymer film comprises a base layer formed from a hydrophilic material as the central portion, and a secondary layer formed of a hydrophobic material as the exterior portion.

17. The sample holder of claim 16, wherein a void is etched in the secondary layer to expose the hydrophilic material of the base layer, thereby forming a hydrophilic central portion surrounded by a hydrophobic exterior portion.

18. The sample holder of claim 1, wherein the support surface comprises a polymer film having a base layer formed from a hydrophobic material as the central portion and a secondary layer formed of a hydrophilic material as the exterior portion.

19. The sample hold of claim 18, wherein a void is etched in the secondary layer to expose the hydrophobic material of the base layer, thereby forming a hydrophobic central portion surrounded by a hydrophilic exterior portion.

20. A sample holder for retaining a liquid sample in a localized region and configured for subsequent analytical processing in a beam of electromagnetic radiation, the sample holder comprising:

a substantially planar sample support surface for providing a localized region for receiving a test sample and having a substantially planar base layer and a substantially planar secondary layer disposed on the base layer to provide a central portion and an exterior portion substantially surrounding the central portion, one of the base or secondary layers having a hydrophilic surface, and the other of the base or secondary layers having a hydrophobic material such that one of the central or exterior portions has a hydrophilic surface, and the other of the central or exterior portions has a hydrophobic surface, the base and secondary layers being formed of a material which resists spectral contamination.

21. The sample holder of claim 20, wherein the secondary layer has a hole formed therein exposing a portion of the base layer such that the secondary layer forms the exterior portion and the base layer forms the central portion.

22. The sample holder of claim 20, wherein the base layer is a generally hydrophilic polymer film and the second layer is a generally hydrophobic coating disposed on the hydrophilic polymer film;

and further comprising a hole formed in the hydrophobic coating of the secondary layer to expose a portion of the hydrophilic base layer.

23. The sample holder of claim 20, wherein the base layer is a generally hydrophobic film and the second layer is a generally hydrophilic film disposed on the hydrophobic film;

and further comprising a hole formed in the hydrophilic film of the secondary layer to expose a portion of the hydrophobic base layer.

24. The sample holder of claim 20, wherein the base layer is a generally hydrophilic film and the second layer is a generally hydrophobic film disposed on the hydrophilic film, the hydrophobic film forming the exterior portion;

and further comprising a tertiary layer disposed on the secondary layer and forming the central portion with the secondary layer surrounding the tertiary layer, the tertiary layer being a generally hydrophilic film.

25. The sample holder of claim 20, wherein the secondary layer is disposed on the base layer with the base layer surrounding the secondary layer such that the base layer forms the exterior portion and the secondary layer forms the central portion.

26. The sample holder of claim 20, wherein the base layer is a generally hydrophilic film and the second layer is a generally hydrophobic film disposed on the hydrophilic polymer film with the hydrophilic film of the base layer surrounding the hydrophobic film of the secondary layer.