Reaction vessel for heat-exchanging chemical processes

• US 5,958,349 A
• Filed: 02/28/1997
• Issued: 09/28/1999
• Est. Priority Date: 02/28/1997
• Status: Expired due to Term

• Current Assignee: Cepheid (Danaher Corporation)
• Original Assignee: Cepheid (Danaher Corporation)
• Inventors: Kovacs, Gregory T. A., Petersen, Kurt E., McMillan, William A., Young, Steven J.
• Primary Examiner(s): Tran, Hien
• Assistant Examiner(s): Kennedy, James
• Application Number:
  US08/808,325
• Time in Patent Office: 942 Days
• Field of Search: 422/102, 422/52, 422/58, 422/68.1, 422/82.05, 422/82.08, 422/198, 250/238, 356/246, 356/340, 356/349, 356/442
• US Class Current: 422/198
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00873 Heat exchange
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/0332 with temperature control control of temperature G05D23/00; cryostats F17C3/08

Abstract

A reaction vessel for holding a sample for a heat-exchanging chemical process has two opposing major faces and a plurality of contiguous minor faces joining the major faces to each other. The major and minor faces form an enclosed chamber having a triangular-shaped bottom portion. The ratio of the thermal conductance of the major faces to that of the minor faces is at least 2:1, and the minor faces forming the triangular-shaped bottom portion of the chamber are optically transmissive. The vessel also has a port for introducing a sample into the chamber and a cap for sealing the chamber.

Claims

1. A reaction vessel comprising:

a) two opposing major faces for mating with heating or cooling surfaces;

b) a plurality of minor faces, wherein each of the minor faces joins the major faces to each other to form a chamber having a triangular-shaped bottom portion;

c) a port for introducing the sample into the chamber; and

d) means for sealing the port;

wherein the ratio of the thermal conductance of the major faces to that of the minor faces is at least 2;

1, and the minor faces forming the triangular-shaped bottom portion are optically transmissive.

2. The reaction vessel of claim 1, wherein the ratio of the total surface area of the major faces to the total surface area of the minor faces is at least 2:

1.

3. The reaction vessel of claim 1, wherein the minor faces forming the bottom portion of the chamber are angularly offset from each other by an angle of about 90°

.

4. The reaction vessel of claim 1, further comprising an optical element on at least one of the minor faces forming the bottom portion of the chamber.

5. The reaction vessel of claim 4, wherein the optical element comprises a lens molded into the surface of the minor face.

6. The reaction vessel of claim 1, further comprising at least one optical element on each of the minor faces forming the bottom portion of the chamber.

7. The reaction vessel of claim 1, wherein each of the minor faces forming the bottom portion of the chamber has at least one lens molded into its surface.

8. The reaction vessel of claim 1, wherein the minor faces forming the bottom portion of the chamber are fabricated from a different material than the other faces.

9. The reaction vessel of claim 1, wherein the chamber has a volume capacity in the range of 10 to 100 microliters.

10. The reaction vessel of claim 1, wherein the minor faces forming the top portion of the chamber are provided by an insert fitted into the vessel.

11. The reaction vessel of claim 10, wherein the insert further defines the port and a channel connecting the port to the chamber.