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Protein activity screening of clones having DNA from uncultivated microorganisms

  • US 5,958,672 A
  • Filed: 06/03/1996
  • Issued: 09/28/1999
  • Est. Priority Date: 07/18/1995
  • Status: Expired due to Term
First Claim
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1. A method for identifying a protein activity of interest comprising:

  • culturing a gene expression library comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor organisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism; and

    detecting the protein activity encoded by the cDNA or genomic DNA fragments.

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