Probes for papillomaviruses and an in vitro diagnostic procedure for papilloma infections
First Claim
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1. A purified DNA of human papillomaviruses, which is a gene selected from the group consisting of E1, E6-E7, L1, and L2,wherein said human papillomavirus is selected from the group consisting of HPV-IP5 and HPV-IP6.
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Abstract
The invention relates to papillomaviruses, particularly to DNA-HPVs isolated from the papillomaviruses IP5 and IP6, the restriction maps of papillomaviruses IP5 and IP6, and also to probes containing these DNA-HPVs or fragments obtained from them. The invention relates, in addition, to "kits" containing distinct groups of probes, containing one of these DNA-HPVs or DNA-HPV fragments, as well as a procedure for the detection and identification of papillomaviruses which makes use of these different probes.
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25 Claims
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1. A purified DNA of human papillomaviruses, which is a gene selected from the group consisting of E1, E6-E7, L1, and L2,
wherein said human papillomavirus is selected from the group consisting of HPV-IP5 and HPV-IP6.
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2. A purified DNA, which hybridizes to the DNA of a human papillomavirus (HPV) selected from the group consisting of HPV-IP5 and HPV-IP6.
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5. A probe for the detection of human papillomavirus (HPV) DNA, which hybridizes under stringent conditions with the genome of a human papillomavirus selected from the group consisting of HPV-IP5 and HPV-IP6.
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8. A kit comprising a plurality of different probes for detecting human papillomavirus (HPV) DNA, wherein said kit contains at least one probe selected from each of the following groups a-k:
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a) DNA of HPV2d; b) DNA of HPV10b, 28 and 29; c) DNA of HPV17, 24; d) DNA of HPV14, 15, 17, 19, 20, 21, 22, 23 and IP4; e) DNA of HPV15 and 17; f) DNA of HPV24; g) DNA of HPV14, 32 and IP4; h) DNA of HPV31; i) DNA of HPV32; j) DNA of HPV16, 18, IP2 and IP5; and k) DNA of HPV6, 11 and IP6, wherein each of the probes in said kit is of a different HPV type from the other probes in said kit.
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9. A kit for the in vitro detection of infection by human papillomavirus, said kit comprising DNA probes of human papillomavirus types in a mixture selected from the following groups a-k:
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a) DNA of HPV2d; b) DNA of HPV10b, 28 and 29; c) DNA of HPV17, 24; d) DNA of HPV14, 15, 17, 19, 20, 21, 22, 23 and IP4; e) DNA of HPV15 and 17; f) DNA of HPV24; g) DNA of HPV14, 32 and IP4; h) DNA of HPV31; i) DNA of HPV32; j) DNA of HPV16, 18, IP2 and IP5; and k) DNA of HPV6, 11 and IP6.
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10. A purified recombinant DNA molecule produced by the process comprising cloning a vector in a host cell, wherein said vector comprises human papillomavirus DNA selected from the HPV group consisting of HPV IP5 and HPV IP6.
- 11. A purified recombinant DNA molecule produced by a process comprising cloning a vector in a host cell, wherein said vector comprises human papillomavirus IP5 DNA.
- 18. A cloned recombinant DNA molecule produced by a process comprising cloning a vector in a host cell, wherein said vector comprises human papillomavirus IP6 DNA.
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25. A kit for the in vitro detection of infection by human papillomavirus, said kit comprises eleven DNA probes of human papillomavirus types in a mixture, wherein said DNA probes are from the human papillomaviruses selected from the following groups a-k:
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a) HPV1, 2d and 4; b) HPV3, 10a, 10b, 28, and 29; c) HPV5, 17a, and 24; d) HPV5, 8, 12, 14a, 14b, 19, 20, 21, 22, 23, and IP4; e) HPV9, 15, 17a, and 17b; f) HPV24; g) HPV5, 8, 14b, 32, and IP4; h) HPV13 and 31; i) HPV32 and 1P4; j) HPV16, 18, IP2, and IP5; and k) HPV6, 11, and IP6.
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Specification