Use of melatonin to prevent cytotoxic effects of amyloid beta protein

• US 5,958,964 A
• Filed: 02/18/1997
• Issued: 09/28/1999
• Est. Priority Date: 02/18/1997
• Status: Expired due to Term

• Current Assignee: South Alabama Medical Science Foundation
• Original Assignee: South Alabama Medical Science Foundation
• Inventors: Pappolla, Miguel A.
• Primary Examiner(s): Tsang, Cecilia J.
• Assistant Examiner(s): Lukton, David
• Application Number:
  US08/801,301
• Time in Patent Office: 952 Days
• Field of Search: 514/415, 514/292, 548/469
• US Class Current: 514/415
• CPC Class Codes: A61K 31/4045 Indole-alkylamines; Amides thereof, e.g. serotonin, melatonin

Abstract

The present invention is directed to a method of preventing cytotoxic effects of amyloid beta protein on cells. The method comprises exposing the cells to an effective amount of melatonin. The invention further provides a method of treating Alzheimer'"'"'s disease in a human subject, which comprises administering an amount of melatonin effective to prevent the cytotoxic effects of amyloid beta protein to the human subject.

Claims

1. A method of enhancing the survivability of cells that have been subjected to cytotoxic effects of amyloid beta protein comprising exposing said cells to an effective amount of melatonin.

2. The method of claim 1 wherein the exposure to melatonin results in a decrease in incidence of cell death.

3. The method of claim 1 where said cytotoxic effect is increased lipid peroxidation.

4. The method of claim 1 where said cytotoxic effect is increased intracellular Ca²⁺.

5. The method of claim 1 wherein said cytotoxic effect is increased oxygen-centered free radicals.

6. The method of claim 1 wherein said cells are neuronal cells.

7. The method of claim 1 wherein said cells are present in a human subject and said exposing results from systemic administration of said melatonin.

8. The method of claim 7 wherein said effective amount is between about 1 μ

g and about 100 g melatonin.

9. The method of claim 1 wherein said cells are present in a human subject having Alzheimer'"'"'s disease.

10. The method of claim 1 wherein said effective amount is between about 1 and 100 μ

M melatonin.

11. A method of enhancing the survivability of cells that have been subjected to cytotoxic effects of amyloid beta protein in a patient afflicted with Alzheimer'"'"'s disease, comprising administering melatonin to the patient in an amount effective to alleviate symptoms associated with said disease.

12. The method of claim 11 wherein the amount is between about 1 μ

g and about 100 g melatonin.

13. The method of claim 11 wherein said administering is systemic.