Supports useful for molecular imprinting technology

• US 5,959,050 A
• Filed: 07/05/1996
• Issued: 09/28/1999
• Est. Priority Date: 05/26/1995
• Status: Expired due to Term

• Current Assignee: Klaus Mosbach
• Original Assignee: Andrew G. Mayes, Klaus Mosbach
• Inventors: Mosbach, Klaus, Mayes, Andrew G.
• Primary Examiner(s): Lipman, Bernard
• Assistant Examiner(s): SAROFIM, NABIL F
• Application Number:
  US08/675,979
• Time in Patent Office: 1,180 Days
• Field of Search: 526/317.1, 526/201, 526/199, 526/200, 526/215, 526/243, 526/318.43, 526/326.2, 526/326.3
• US Class Current: 526/201
• CPC Class Codes: B01D 15/3852 using imprinted phases or molecular recognition; using imprinted phases
• CPC Class Codes: B01J 20/26 Synthetic macromolecular compounds
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: C07B 57/00 Separation of optically-active compounds
• CPC Class Codes: C08F 2/20 with the aid of macromolecular dispersing agents
• CPC Class Codes: C08F 2/44 Polymerisation in the presence of compounding ingredients, e.g. plasticisers, dyestuffs, fillers
• CPC Class Codes: C08F 220/286 and containing polyethylene oxide in the alcohol moiety, e.g. methoxy polyethylene glycol (meth)acrylate
• CPC Class Codes: C08F 220/387 and containing nitrogen and oxygen
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template

Abstract

The claimed invention is directed to a molecularly imprinted support formed from at least two distinct acylic monomers and at least one imprinted molecule. The support comprises beads having a uniform surface for reproducible presentation thereon.

Claims

1. A molecularly imprinted support comprising substantially spherical, uniformly shaped, and non-aggregated particles formed by suspension polymerization of at least two distinct acrylic monomers in the presence of at least one imprint molecule, at least one liquid fluorocarbon compound, and at least one fluorocarbon copolymer.

2. A support according to claim 1, wherein said beads have a diameter of from about 2 to 100 microns.

3. A support according to claim 2, wherein said beads have a diameter of between 2 and 50 microns.

4. A support according to claim 3, wherein said beads have a diameter of between about 5 and 25 microns.

5. A support according to claim 1, wherein said acrylic monomers are methacrylic acid and ethylene glycol dimethacrylate.

6. A support according to claim 1, wherein said molecular imprint molecule is selected from the group consisting of amino-acids, amino-acid derivatives, pharmaceutical compounds, and polysaccharides.

7. A support according to claim 6, wherein said molecular imprint molecule is selected from the group consisting of D-Boc-amino acids, L-Boc-amino acids, and derivatives of D- and L-Boc-amino acids.

8. A support according to claim 1, wherein said stabilizer is an acrylate polymer.

9. A support according to claim 1, wherein said stabilizer has perfluorocarbon groups and polyoxethylene ester groups.

10. A molecularly imprinted polymer in spherical form comprising crosslinked polyacrylate which has been prepared by suspension polymerization of at least two acrylate monomers in the presence of a fluorinated solvent and a fluorinated copolymer.

11. A molecularly imprinted polymer according to claim 10, wherein the polymer is isolated after suspension polymerization without use of grinding or sieving.

12. A molecularly imprinted polymer according to claim 10, wherein a porogenic solvent is also present in the suspension polymerization.

13. A molecularly imprinted polymer according to claim 10, wherein the fluorinated copolymer comprises ester groups.

14. A molecularly imprinted polymer according to claim 12, wherein the fluorinated copolymer comprises ester groups.

15. A molecularly imprinted polymer prepared by the combination of steps consisting essentially of:

(i) providing a polymerizable composition mixture for suspension polymerization including at least two acrylate monomers, at least one porogenic solvent, at least one fluorine-containing liquid, at least one fluorine-containing copolymer, and at least one print molecule;

(ii) executing suspension polymerization on the polymerizable composition mixture to yield substantially spherical polymer particles;

(iii) isolating the spherical particles from the porogenic solvent and the fluorine-containing liquid; and

(iv) removing the print molecule from the spherical particles.

16. A molecularly imprinted polymer according to claim 15, wherein the fluorine-containing liquid is a perfluorinated liquid.

17. A molecularly imprinted polymer according to claim 16, wherein the fluorine-containing copolymer includes ester groups.