Process for stabilizing the content of glycated protein of a sample on a matrix material

• US 5,959,076 A
• Filed: 08/26/1996
• Issued: 09/28/1999
• Est. Priority Date: 08/24/1995
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Original Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Inventors: Nagel, Rolf, Mistele, Jurgen
• Primary Examiner(s): Tsang, Cecilia J.
• Assistant Examiner(s): Gupta, Anish
• Application Number:
  US08/702,972
• Time in Patent Office: 1,128 Days
• Field of Search: 514/2, 514/8, 530/322, 530/350
• US Class Current: 530/322
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92
• CPC Class Codes: G01N 33/6842 Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
• CPC Class Codes: G01N 33/723 Glycosylated haemoglobin
• CPC Class Codes: G01N 33/726 Devices

Abstract

The invention concerns a method for stabilizing the content of glycated protein in a sample on a matrix material, which is characterized in that the matrix material is impregnated with boric acid buffer with a pH which is larger or equal to 10.5 or a transition metal salt as well as an appropriate matrix material, an element for collecting, transporting and storing sample material to be analysed with such a matrix material and a system containing such an element and a sealable covering.

Claims

1. A method for stabilizing a glycated protein comprising applying said glycated protein to a matrix material which has been impregnated with a member selected from the group consisting of boric acid, and a water soluble transition metal salt wherein said boric acid has a pH of at least 10.5.

2. The method according to claim 1, wherein said boric acid has a pH of at least about 11.

3. The method according to claim 1, wherein said water soluble transition metal salt is selected from the group consisting of a nickel salt and a copper salt.