Integrated optic waveguide immunosensor

• US 5,961,924 A
• Filed: 10/31/1997
• Issued: 10/05/1999
• Est. Priority Date: 02/23/1995
• Status: Expired due to Term

• Current Assignee: University of Utah Research Foundation (State of Utah)
• Original Assignee: University of Utah Research Foundation (State of Utah)
• Inventors: Christensen, Douglas A., Wang, Hsu-Kun, Herron, James N., Reichert, W. Monty
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US08/961,827
• Time in Patent Office: 704 Days
• Field of Search: 422/68, 422/82.11, 422/82.05-82.1, 385/12
• US Class Current: 422/82.11
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A step-gradient composite waveguide for evanescent sensing in fluorescent binding assays comprises a thick substrate layer having one or more thin film waveguide channels deposited thereon. In one embodiment, the substrate is silicon dioxide and the thin film is silicon oxynitride. Specific binding molecules having the property of binding with specificity to an analyte are immobilized on the surface of the thin film channels. In preferred embodiments, the composite waveguide further includes light input coupling means integrally adapted to the thin film channels. Such light coupling means can be a grating etched into the substrate prior to deposition of the thin film, or a waveguide coupler affixed to the upper surface of the thin film. The waveguide coupler has a thick input waveguide of high refractive index which receives the laser light through one end and propagates it by total internal reflection. The propagated light is then coupled evanescently into the thin film waveguide across a spacer layer of precise thickness and having an index of refraction lower than either the input waveguide or the thin-film waveguide. The composite waveguide can be constructed by plasma vapor deposition of silicon oxynitride onto the silicon dioxide substrate, masking the channel waveguides with a photoresist, and using reactive ion etching to expose the substrate in the unmasked regions.

Claims

1. An optical structure for performing specific binding assays with an optical unit comprising a light source positioned to direct light into a waveguide for propagation by total internal reflectance in said waveguide and a detector oriented to detect light from a region proximal to the optical structure, said optical structure comprising:

a substrate comprising a first optical material of refractive index n₁ and having two parallel planar surfaces separated by a thickness and a surrounding edge, the thickness being between about 0.3 μ

m and about 10 mm;

a waveguide film of a second optical material of refractive index n₂ different from n₁ in direct contact with at least a portion of one of said two parallel planar surfaces of said substrate, said waveguide film having a thickness which is between about 0.3 μ

m and about 5 μ

m, and said waveguide film further having a plurality of specific binding molecules associated therewith; and

coupling means for coupling light into said waveguide film, said coupling means directly contacting said waveguide film.

2. The optical structure of claim 1, wherein said second optical material is selected from the group consisting of:

silicon oxynitride of refractive index n₂ and silicon dioxide; and

said first optical material is selected from the group consisting of;

pure silicon dioxide, quartz or fused silica, silicon oxynitride of refractive index n₁, and magnesium fluoride.

3. The optical structure of claim 2, wherein said coupling means is selected from the group consisting of:

an optical grating formed of said first material;

an optical grating formed of said second material; and

a waveguide coupler integrally mounted on said waveguide film, said waveguide coupler comprising

an input waveguide constructed to receive light through an edge and to propagate the received light by total internal reflection, and

a spacing layer interposed between said input waveguide and said waveguide film and having a thickness selected to optimize the evanescent coupling of light from said input waveguide into said waveguide film.

4. The optical structure of claim 3, wherein said first optical material is quartz, fused silica or deposited SiO₂, said coupling means is said waveguide coupler, and wherein said input waveguide is formed of an optical material selected from the group consisting of:

rutile, zirconia, and high-index glass; and

wherein said spacing layer is formed of silicon dioxide and has a thickness of between about 0.1 μ

m and about 5 μ

m.

5. The optical structure of claim 1, wherein said waveguide film is configured as a plurality of strips of said second optical material adhered to one of said two parallel planar surfaces in a spaced parallel array.

6. The optical structure of claim 1, further comprising a plurality of sample wells formed thereon, said sample wells formed by the steps of:

epitaxially depositing an outer layer of refractive index n₁ upon said waveguide film;

applying a resist compound to a plurality of outlines which define said sample wells while leaving areas within and without the outlines uncoated; and

removing said outer layer by etching to expose said waveguide film in said uncoated areas.