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Method and apparatus for cell counting and cell classification

  • US 5,962,238 A
  • Filed: 09/16/1996
  • Issued: 10/05/1999
  • Est. Priority Date: 02/17/1993
  • Status: Expired due to Term
First Claim
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1. A method of detecting peak events indicative of target components in a fluid sample contained in a sample holder, the method comprising:

  • positioning an objective lens at a plurality of locations in a first direction along the sample;

    focusing the objective lens in a second direction substantially perpendicular to the first direction at each of the plurality of locations to find a position of optimal fluorescence in the second direction corresponding to each of the plurality of locations;

    scanning the sample in at least the first direction to detect peak events while adjusting the objective lens in the second direction to a focal length that is determined from at least one position of optimal fluorescence.

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