×

Quantitative determination method for chloride ions

  • US 5,962,248 A
  • Filed: 01/23/1996
  • Issued: 10/05/1999
  • Est. Priority Date: 08/04/1993
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for quantitatively determining chloride ion in a sample, comprising the steps of:

  • (1) admixing (i) an enzyme having a glucokinase activity, (ii) adenosine triphosphate and (iii) an aqueous medium comprising said sample;

    (2) reacting said sample with said enzyme to eliminate any glucose coexisting in said sample, to obtain mixture (A);

    (3) admixing mixture (A) with (i) an α

    -amylase which has been deactivated by a chelating agent and (ii) a chelating agent to deactivate the enzyme having a glucokinase activity, to obtain mixture (B);

    (4) reacting said α

    -amylase of mixture (B) with a naturally occurring oligosaccharide selected from the group consisting of maltotetraose, maltohexaose and maltoheptaose;

    (5) determining an amount of glucose produced in the reaction of step (4); and

    (6) correlating said amount of glucose with a quantity of chloride ion in said sample.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×