Quantitative determination method for chloride ions
First Claim
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1. A method for quantitatively determining chloride ion in a sample, comprising the steps of:
- (1) admixing (i) an enzyme having a glucokinase activity, (ii) adenosine triphosphate and (iii) an aqueous medium comprising said sample;
(2) reacting said sample with said enzyme to eliminate any glucose coexisting in said sample, to obtain mixture (A);
(3) admixing mixture (A) with (i) an α
-amylase which has been deactivated by a chelating agent and (ii) a chelating agent to deactivate the enzyme having a glucokinase activity, to obtain mixture (B);
(4) reacting said α
-amylase of mixture (B) with a naturally occurring oligosaccharide selected from the group consisting of maltotetraose, maltohexaose and maltoheptaose;
(5) determining an amount of glucose produced in the reaction of step (4); and
(6) correlating said amount of glucose with a quantity of chloride ion in said sample.
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Abstract
Chloride ion is quantitatively determined in a sample by adding to the sample an enzyme having glucokinase activity to thereby eliminate glucose, admixing an α-amylase which has been deactivated by a chelating agent, and reacting the α-amylase with maltotetraose, maltohexaose or maltoheptaose.
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Citations
9 Claims
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1. A method for quantitatively determining chloride ion in a sample, comprising the steps of:
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(1) admixing (i) an enzyme having a glucokinase activity, (ii) adenosine triphosphate and (iii) an aqueous medium comprising said sample; (2) reacting said sample with said enzyme to eliminate any glucose coexisting in said sample, to obtain mixture (A); (3) admixing mixture (A) with (i) an α
-amylase which has been deactivated by a chelating agent and (ii) a chelating agent to deactivate the enzyme having a glucokinase activity, to obtain mixture (B);(4) reacting said α
-amylase of mixture (B) with a naturally occurring oligosaccharide selected from the group consisting of maltotetraose, maltohexaose and maltoheptaose;(5) determining an amount of glucose produced in the reaction of step (4); and (6) correlating said amount of glucose with a quantity of chloride ion in said sample. - View Dependent Claims (2, 3, 5, 6, 7, 8, 9)
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4. The method of claim 4, wherein said enzyme having a glucokinase activity is hexokinase type IV.
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