Antibodies to protein, FAF1

• US 5,962,652 A
• Filed: 12/18/1997
• Issued: 10/05/1999
• Est. Priority Date: 06/07/1995
• Status: Expired due to Fees

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Chu, Keting, Williams, Lewis T.
• Primary Examiner(s): MERTZ, PREMA MARIA
• Application Number:
  US08/993,210
• Time in Patent Office: 656 Days
• Field of Search: 530/387.1, 530/387.9, 530/388.1, 530/350, 435/325, 435/326, 435/7.1
• US Class Current: 530/387.9
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C07K 14/4747 Apoptosis related proteins
• CPC Class Codes: C07K 2319/00 Fusion polypeptide

Abstract

The present invention identifies a novel, Fas-associated factor 1 termed FAF1 which potentiates Fas-induced cell killing. The invention provides FAF1 nucleic acid and polypeptide compositions as well as methods of using these compositions in the therapeutic treatment of diseases resulting from dysregulation in apoptosis. Also provided are cells carrying and expressing the nucleic acid compositions and methods of using these cells to screen for agonists and antagonists of Fas-mediated apoptosis. Methods of isolating FAF1-interacting proteins are disclosed. Also provided are antibodies that bind FAF1, a hybridoma and a kit comprising the antibodies.

Claims

1. An antibody that specifically binds to an isolated polypeptide comprising a domain of a Fas-associated factor 1 (FAF1) polypeptide, said polypeptide capable of associating with a cytoplasmic domain of Fas, wherein said FAF1 domain is encoded by a nucleic acid sequence that comprises at least 18 nucleotides and hybridizes to the complementary nucleic acid sequence shown in SEQ ID NO:

1 or to a degenerate form thereof.

2. The antibody of claim 1, wherein the polypeptide comprises the sequence of SEQ ID NO:

2.

3. A kit comprising an antibody of claim 1.

4. The antibody of claim 1, which is a rabbit antiserum or a monoclonal antibody.

5. A hybridoma capable of producing the monoclonal antibody of claim 4.