Method and apparatus for displaying capillary electrophoresis data

• US 5,963,456 A
• Filed: 11/27/1996
• Issued: 10/05/1999
• Est. Priority Date: 07/17/1992
• Status: Expired due to Term

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: Beckman Instruments, Inc. (Danaher Corporation)
• Inventors: Klein, Gerald L., Katzmann, Steven P.
• Primary Examiner(s): Weinhardt, Robert A.
• Assistant Examiner(s): Thomas, Joseph
• Application Number:
  US08/757,498
• Time in Patent Office: 1,042 Days
• Field of Search: 364/496, 364/497, 364/498, 364/526, 364/FOR 117, 364/FOR 114, 364/FOR 115, 356/394, 395/202, 395/203, 345/107, 345/88, 345/89, 345/90, 345/131, 430/32, 430/34, 430/38, 204/451, 204/455, 204/456, 204/600, 204/601, 204/603, 204/604, 204/606
• US Class Current: 702/19
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

A method and apparatus for displaying capillary electrophoresis data wherein absorbance with respect to time is related to graphic values within a graphic range or scale of values and selected graphic values are displayed with respect to time. The selected graphic values may comprise a range hues, color saturation and/or brightness or may represent a monochromatic scale. The resulting representation may be horizontally aligned with the corresponding electrophoretogram absorbance values to provide both a quantitative value (electrophoretogram absorbance values) and qualitative (graphic stripe) display.

Claims

1. A method of displaying capillary electrophoretograms, the method including:

(a) electrophoresing a biological fluid in a capillary, the fluid comprising a plurality of biological constituents;

(b) passing a light beam through the fluid at a predetermined position into the capillary;

(c) detecting with respect to time a plurality of quantitative absorbance values related to the absorbance of the light beam by the biological constituents of the fluid in the capillary;

(d) retaining the detected absorbance values in a memory device;

(e) selecting a range of color characteristic values;

(f) selecting a range of absorbance values;

(g) correlating the range of color characteristic values to the range of absorbance values;

(h) relating each of the retained absorbance values to the range of absorbance values;

(i) selecting a color characteristic value from the range of color characteristic values for each retained absorbance value; and

(j) displaying the selected color characteristic values as a stripe pattern including a plurality of stripes, the stripe pattern having an axis related to time and the stripes being spaced from each other on the axis, the color characteristic values of the stripes correlating to the retained absorbance values with respect to time, wherein (i) the stripes qualitatively represent the concentration of the biological constituents in the fluid, and (ii) different stripes have the same or have different color characteristic values.

2. A method as in claim 1 wherein displaying the color characteristic values includes displaying the values on a computer display screen.

3. A method as in claim 1 wherein displaying the color characteristic values includes printing the values.

4. A method as in claim 1 wherein the range of color characteristic values is a range of varying color.

5. The method of claim 1 wherein the step of displaying comprises:

(a) displaying on a display the absorbance values as a graph having absorbance value on a vertical axis and time on a horizontal axis; and

(b) displaying the stripe pattern on the same display, the stripe pattern having a horizontal axis related to time, wherein there is a one-to-one correspondence between absorbance values displayed in the graph and absorbance values displayed in the stripe pattern.

6. A method as in claim 1 wherein the range of color characteristic values comprises a range of hues or shades of gray.

7. A method as in claim 1 wherein the stripes of the stripe pattern have the same height on the axis.

8. A method as in claim 1 wherein the stripes of the stripe pattern each have multiple color characteristic values.

9. A method of displaying capillary electrophoresis electrophoretograms, the method including:

(a) concurrently performing electrophoresis of biological fluids in a plurality of capillaries, each of the biological fluids comprising a plurality of biological constituents;

(b) passing a light beam through the fluid at a predetermined position into each capillary;

(c) detecting with respect to time a plurality of quantitative absorbance values related to the absorbance of the light beam by the biological constituents of the fluid in each of the capillaries;

(d) retaining the detected absorbance values for each capillary in a memory device;

(e) selecting a range of color characteristic values for each capillary;

(f) selecting a range of absorbance values for each capillary;

(g) correlating the range of color characteristic values to the range of absorbance values for each capillary;

(h) relating each of the retained absorbance values to the range of absorbance values for each capillary;

(i) selecting a color characteristic value from the range of color characteristic values for each retained absorbance value for each capillary; and

(j) displaying the selected color characteristic values for each of the capillaries as a stripe pattern including a plurality of stripes, each stripe pattern having an axis related to time and the stripes being spaced from each other on the axis, the color characteristic values of the stripes correlating to the retained absorbance values with respect to time, wherein (i) the stripes qualitatively represent the concentration of the biological constituents in the fluid, and (ii) different stripes have the same or have different color characteristic values.

10. A method as in claim 9 wherein displaying the color characteristic values includes displaying the values on a computer display screen.

11. A method as in claim 9 wherein displaying the color characteristic values includes printing the values.

12. A method as in claim 9 wherein the stripes are aligned horizontally with respect to time.

13. A method as in claim 9 wherein the range of color characteristic values is monochromatic.

14. The method of claim 9 wherein the step of displaying comprises:

(a) displaying on a display the retained absorbance values for a capillary as a graph having absorbance value on a vertical axis and time on a horizontal axis; and

(b) displaying the stripe pattern on the same display, the stripe pattern having a horizontal axis related to time, wherein there is a one-to-one correspondence between absorbance values displayed in the graph and absorbance values displayed in the stripe pattern.

15. A method as in claim 9 wherein the stripes of the stripe pattern have the same height on the axis.

16. A method as in claim 9 wherein the stripes of the stripe pattern each have multiple color characteristic values.

17. A method for displaying capillary electrophoresis measurements, the method including:

(a) electrophoresing a biological liquid in a capillary, the liquid comprising a plurality of biological constituents;

(b) passing a light beam through the liquid at a predetermined position into the capillary;

(c) detecting with respect to time the quantitative absorbance of the light beam by the biological constituents of the liquid during at least a portion of the electrophoresising time;

(d) retaining the detected absorbance in a memory device;

(e) selecting a range of color characteristic values;

(f) selecting a range of absorbance values;

(g) correlating the range of color characteristic values to the range of absorbance values;

(h) relating the retained absorbance detected at predetermined times to the range of absorbance values;

(i) selecting a color characteristic value from the range of color characteristic values for each predetermined time; and

(j) displaying the selected color characteristic values as a stripe pattern including a plurality of stripes, the stripe pattern having an axis related to time and the stripes being spaced from each other on the axis, the color characteristic values of the stripes correlating to the retained absorbance with respect to time, wherein (i) the stripes qualitatively represent the concentration of the biological constituents in the fluid, and (ii) different stripes have the same or have different color characteristic values.

18. A method as in claim 17 wherein the step of displaying includes displaying the selected color characteristic values as electrophoresising occurs.

19. A method as in claim 17 wherein step (h) comprises determining a ratio according to the value of the absorbance within the range of absorbance values, and step (i) comprises selecting the color characteristic value according to the ratio.

20. A method as in claim 19 wherein the range of absorbance values has an upper limit and a lower limit.

21. A method as in claim 20 wherein the method includes varying the upper limit.

22. A method as in claim 19 wherein the method includes selecting maximum and minimum absorbance values from the absorbance values detected and setting the range of absorbance values to the maximum and minimum values.

23. A method as in claim 17 wherein the selected color characteristic values are displayed with respect to electrophoresising time.

24. A method as in claim 23 wherein the method includes displaying the detected absorbance as a graph having absorbance value on a vertical axis and time on a horizontal axis.

25. A method as in claim 24 wherein the selected color characteristic values time axis and the graph time axis are horizontally aligned.

26. A method as in claim 25 wherein the stripe pattern axis corresponds to electrophoresing time.

27. A method as in claim 26 wherein the graph is displayed on the stripe pattern.

28. A method as in claim 26 wherein there is a one-to-one correspondence between absorbance values displayed in the graph and absorbance values displayed in the stripe pattern.

29. A method as in claim 17 wherein the range of color characteristic values is nonlinear.

30. A method as in claim 17 wherein the range of color characteristic values is linear.

31. A method as in claim 17 wherein the range of color characteristic values is visually differing from one end of the range to the other end of the range.

32. A method as in claim 17 wherein the stripes of the stripe pattern have the same height on the axis.

33. A method as in claim 17 wherein the stripes of the stripe pattern each have multiple color characteristic values.

34. A capillary electrophoresis apparatus, the apparatus comprising:

(a) means for electrophoresing a biological fluid in a capillary, the fluid comprising a plurality of biological constituents;

(b) means for passing a light beam through the fluid at a predetermined position into the capillary;

(c) means for detecting with respect to time a plurality of quantitative absorbance values related to the absorbance of the light beam by the biological constituents of the fluid in the capillary;

(d) means for retaining the detected absorbance values;

(e) means for selecting at least one range of color characteristic values;

(f) means for selecting a range of absorbance values;

(g) means for correlating the range of color characteristic values to the range of absorbance values;

(h) means for relating each of the retained absorbance values to the range of absorbance values;

(i) means for selecting a color characteristic value from the range of color characteristic values for each retained absorbance value; and

(j) means for displaying the selected color characteristic values as a stripe pattern including a plurality of stripes, the stripe pattern having an axis related to time and the stripes being spaced from each other on the axis, the color characteristic values of the stripes correlating to the retained absorbance values with respect to time, wherein (i) the stripes qualitatively represent the concentration of the biological constituents in the fluid, and (ii) different stripes have the same or have different color characteristic values.

35. An apparatus as in claim 34 wherein the displaying means includes means for displaying the values on a computer display screen.

36. An apparatus as in claim 34 wherein the means for displaying the color characteristic values includes means for printing the values.

37. An apparatus as in claim 34 wherein the range of color characteristic values is a range of varying color.

38. The apparatus of claim 34 wherein the means for displaying comprises:

(a) means for displaying on a display the retained absorbance values as a graph having absorbance value on a vertical axis and time on a horizontal axis; and

(b) means for displaying the stripe pattern on the same display, the stripe pattern having a horizontal axis related to time, wherein there is a one-to-one correspondence between absorbance values displayed in the graph and absorbance values displayed in the stripe pattern.

39. An apparatus as in claim 34 wherein the stripes of the stripe pattern have the same height on the axis.

40. An apparatus as in claim 34 wherein the stripes of the stripe pattern each have multiple color characteristic values.

41. An electrophoresis apparatus, the apparatus comprising:

(a) means for concurrently performing electrophoresis of biological fluids in a plurality of capillaries, the fluids comprising a plurality of biological constituents;

(b) means for passing a light beam through the fluid at a predetermined position into each capillary;

(c) means for detecting with respect to time a plurality of quantitative absorbance values related to the absorbance of the light beam by the biological constituents of the fluid in each of the capillaries;

(d) means for retaining the detected absorbance values;

(e) means for selecting at least one range of color characteristic values for each capillary;

(f) means for selecting a range of absorbance values for each capillary;

(g) means for correlating the range of color characteristic values to the range of absorbance values for each capillary;

(h) means for relating each of the retained absorbance values to the range of absorbance values for each capillary;

(i) means for selecting a color characteristic value from the range of color characteristic values for each retained absorbance value for each capillary; and

(j) means for displaying the selected color characteristic values for each of the capillaries as a stripe pattern including a plurality of stripes, each stripe pattern having an axis related to time and the stripes being spaced from each other on the axis, the color characteristic values of the stripes correlating to the retained absorbance values with respect to time, wherein (i) the stripes qualitatively represent the concentration of the biological constituents in the fluid, and (ii) different stripes have the same or have different color characteristic values.

42. An apparatus as in claim 41 wherein the means for displaying the color characteristic values includes means for displaying the values on a computer display screen.

43. An apparatus as in claim 41 wherein the means for displaying the color characteristic values includes means for printing the values.

44. An apparatus as in claim 41 wherein the stripes are aligned horizontally with respect to time.

45. An apparatus as in claim 41 wherein the range of color characteristic values is monochromatic.

46. The apparatus of claim 41 wherein the means for displaying comprises:

(a) means for displaying on a display the retained absorbance values for a capillary as a graph having absorbance value on a vertical axis and time on a horizontal axis; and

(b) means for displaying the stripe pattern on the same display, the stripe pattern having a horizontal axis related to time, wherein there is a one-to-one correspondence between absorbance values displayed in the graph and absorbance values displayed in the stripe pattern.

47. An electrophoresis apparatus, the apparatus including:

(a) means for electrophoresing a biological liquid in a capillary, the liquid comprising a plurality of biological constituents;

(b) means for passing a light beam through the liquid at a predetermined position into the capillary;

(c) means for detecting with respect to time the quantitative absorbance of the light beam by the biological constituents of the liquid during at least a portion of the electrophoresising time;

(d) means for retaining the detected absorbance;

(e) means for selecting at least one range of color characteristic values;

(f) means for selecting a range of absorbance values;

(g) means for correlating the range of color characteristic values to the range of absorbance values;

(h) means for relating the retained absorbance values detected at predetermined times to the range of absorbance values;

(i) means for selecting a color characteristic value from the range of color characteristic values for each predetermined time; and

(j) means for displaying the selected color characteristic values as a stripe pattern including a plurality of stripes, the stripe pattern having an axis related to time and the stripes being spaced from each other on the axis, the color characteristic values of the stripes correlating to the retained absorbance with respect to time, wherein (i) the stripes qualitatively represent the concentration of the biological constituents in the fluid, and (ii) different stripes have the same or have different color characteristic values.

48. An apparatus as in claim 47 wherein the means for relating includes means for determining a ratio according to the value of the absorbance within the range of absorbance values, and the means for selecting includes means for selecting the color characteristic value according to the ratio.

49. An apparatus as in claim 48 wherein the means for selecting a range of absorbance values includes means for setting an upper limit and a lower limit for the absorbance values.

50. An apparatus as in claim 49 wherein the means for selecting a range of absorbance values includes means for varying the upper limit.

51. An apparatus as in claim 49 wherein the means for setting the upper and lower limit includes means for selecting maximum and minimum absorbance values from the absorbance values detected and setting the range of absorbance values to the maximum and minimum values.

52. An apparatus as in claim 47 including means for displaying the selected color characteristic values with respect to electrophoresising time.

53. An apparatus as in claim 52 including means for displaying the detected absorbance as a graph having absorbance value on a vertical axis and time on a horizontal axis.

54. An apparatus as in claim 53 wherein the selected color characteristic values time axis and the graph time axis are horizontally aligned.

55. An apparatus as in claim 54 wherein the stripe pattern has a length which corresponds to electrophoresising time.

56. An apparatus as in claim 55 wherein the graph is displayed on the stripe pattern.

57. An apparatus as in claim 52 wherein there is a one-to-one correspondence between absorbance values displayed in the graph and absorbance values displayed in the stripe pattern.

58. An apparatus as in claim 47 wherein the range of color characteristic values is monochromatic.

59. An apparatus as in claim 47 wherein the range of color characteristic values is visually differing from one end of the range to the other end of the range.

60. The apparatus of claim 47 wherein the means for displaying comprises:

(a) means for displaying on a display the retained absorbance as a graph having absorbance value on a vertical axis and time on a horizontal axis; and

(b) means for displaying on the same display, the stripe pattern having a horizontal axis related to time, wherein there is a one-to-one correspondence between absorbance values displayed in the graph and absorbance values displayed in the strip pattern.

61. An apparatus as in claim 47 wherein the stripes of the stripe pattern have the same height on the axis.

62. An apparatus as in claim 47 wherein the stripes of the stripe pattern each have multiple color characteristic values.

63. An electrophoresis apparatus for use with reagent segments wherein the reagent segments have a plurality of wells, the apparatus comprising:

(a) a plurality of capillaries, each capillary having a first end and a second end;

(b) means for simultaneously positioning a first end of each of the capillaries into selected distinct ones of the reagent segment wells;

(c) a manifold defining a common conduit including means for receiving the second end of each of the capillaries into the common conduit and thereby establishing a common liquid path between the second ends of the capillaries;

(d) an electrode in the manifold;

(e) means for passing a light beam through a biological liquid at a predetermined position into each capillary, the liquid comprising a plurality of biological constituents;

(f) means for detecting with respect to time the quantitative absorbance of the light beam by the biological constituents of the liquid in each capillary during at least a portion of the electrophoresising time;

(g) means for retaining the detected absorbance;

(h) means for selecting at least one range of color characteristic values for each capillary;

(i) means for selecting a range of absorbance values for each capillary,

(j) means for correlating the range of color characteristic values to the range of absorbance values for each capillary;

(k) means for relating each of the retained absorbance values detected at predetermined times to the range of absorbance values for each capillary;

(l) means for selecting a color characteristic value from the range of color characteristic values for each capillary for each predetermined time; and

(m) means for displaying the selected color characteristic values for each of the capillaries as a stripe pattern including a plurality of stripes, each stripe pattern having an axis related to time and the stripes being spaced from each other on the axis, the color characteristic values of the stripes correlating to the retained absorbance values with respect to time, wherein (i) the stripes qualitatively represent the concentration of the biological constituents in the fluid, and (ii) different stripes have the same or have different color characteristic values.

64. An apparatus as in claim 63 wherein the means for relating includes means for determining a ratio according to the value of the absorbance within the range of absorbance values, and the means for selecting includes means for selecting the color characteristic value according to the ratio.

65. An apparatus as in claim 63 including means for displaying the selected color characteristic values with respect to electrophoresising time.

66. An apparatus as in claim 63 wherein the range of color characteristic values is monochromatic.

67. An apparatus as in claim 63 wherein the stripes of the stripe pattern have the same height on the axis.

68. An apparatus as in claim 63 wherein the stripes of the stripe pattern each have multiple color characteristic values.

69. A method of displaying capillary electrophoresis electrophoretograms including:

(a) electrophoresing a biological fluid in a capillary, the fluid comprising a plurality of biological constituents;

(b) passing a light beam through the fluid at a predetermined position into the capillary;

(c) detecting with respect to time a plurality of quantitative values related to absorbance characteristics of the biological constituents of the fluid in the capillary;

(d) retaining the detected values in a memory device;

(e) selecting a range of color characteristic values;

(f) selecting a range of absorbance values;

(g) correlating the range of color characteristic values to the range of absorbance values;

(h) relating each of the retained quantitative values to the range of absorbance values;

(i) selecting a color characteristic value from the range of color characteristic values for each detected value; and

(j) displaying the selected color characteristic values as a stripe pattern including a plurality of stripes, the stripe pattern having an axis related to time and the stripes being spaced from each other on the axis, the color characteristic values of the stripes correlating to the retained values with respect to time, wherein (i) the stripes qualitatively represent the concentration of the biological constituents in the fluid, and (ii) different stripes have the same or have different color characteristic values.

70. A method as in claim 69 wherein displaying the color characteristic values includes displaying the values on a computer display screen.

71. A method as in claim 69 wherein displaying the color characteristic values includes printing the values.

72. A method as in claim 69 wherein the stripes of the stripe pattern have the same height on the axis.

73. A method as in claim 69 wherein the stripes of the stripe pattern each have multiple color characteristic values.