Glucose sensor

• US 5,964,993 A
• Filed: 12/19/1996
• Issued: 10/12/1999
• Est. Priority Date: 12/19/1996
• Status: Expired due to Term

• Current Assignee: Tenax Therapeutics, Inc.
• Original Assignee: Implanted Biosystems Incorporated
• Inventors: Brunsman, Alan R., Blubaugh, Elmo A. Jr.
• Primary Examiner(s): Beisner, William H.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US08/769,863
• Time in Patent Office: 1,027 Days
• Field of Search: 435/817, 435/180, 435/287.9, 204/403, 205/778, 205/792, 205/793, 205/777.5, 604/20
• US Class Current: 204/403.09
• CPC Class Codes: C12N 11/082 obtained by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C12N 11/089 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A sensor that is capable of accurately measuring glucose and other analytes in low oxygen environments is provided. The sensor employs an enzyme-containing membrane comprising a semi-interpenetrating polymer network which is infiltrated by an enzyme. The enzyme-containing membrane increases oxygen transport to the enzyme such that oxygen is not a limiting factor in the oxidation taking place at the enzyme.

Claims

1. An enzyme-containing membrane comprising a semi-interpenetrating polymer network of fibrillated polytetrafluoroethylene and a silicon compound wherein the network is infiltrated with an enzyme.

2. The membrane of claim 1 wherein the silicon compound is a cross-linked polyorganosiloxane.

3. The membrane of claim 2 wherein the polyorganosiloxane is polydimethylsiloxane.

4. The membrane of claim 1 wherein the enzyme oxidizes glucose and generates hydrogen peroxide.

5. The membrane of claim 4 wherein the enzyme is an oxidase.

6. The member of claim 1 wherein the enzyme is immobilized within said network.

7. The membrane of claim 6 wherein the enzyme is immobilized as an enzyme gel.

8. The membrane of claim 7 wherein the oxidase is glucose oxidase.

9. The membrane of claim 1 wherein the membrane has a porosity of from about 25% to about 55%.

10. The membrane of claim 9 wherein the membrane contains about 15 to 40% by volume of the silicon compound.

12. The membrane system of claim 9 wherein the outer membrane comprises polycarbonate.

13. The membrane system of claim 10 wherein the inner membrane contains about 15 to 40% by volume of said silicon compound.

14. The membrane system of claim 13 wherein the silicon compound of the enzyme-containing membrane is a cross-linked polyorganosiloxane.

15. The membrane system of claim 14 wherein the polyorganosiloxane is polydimethylsiloxane.

16. The membrane system of claim 9 wherein the enzyme of the enzyme-containing membrane is one or a combination of enzymes which oxidizes glucose and generates hydrogen peroxide.

17. The membrane system of claim 16 wherein the enzyme is immobilized in said inner membrane.

18. The membrane system of claim 17 wherein the enzyme is immobilized as an enzyme gel.

19. The membrane system of claim 16 wherein the enzyme is an oxidase.

20. The membrane system of claim 19 wherein the oxidase is glucose oxidase.

21. The membrane system of claim 20 wherein the enzyme-containing membrane has a porosity of from about 25% to about 55%.

11. A membrane system comprising:

an outer membrane; and

an inner membrane, wherein the inner membrane is an enzyme-containing membrane having a semi-interpenetrating polymer network of fibrillated polytetrafluoroethylene and a silicon compound, wherein the network is infiltrated with an enzyme.

22. A glucose sensor comprising:

a membrane system having an outer membrane and an inner membrane, wherein the inner membrane is an enzyme-containing membrane which includes a semi-interpenetrating polymer network of fibrillated polytetrafluoroethylene and a silicon compound, wherein the network is infiltrated by an enzyme, and wherein the outer membrane restricts the flow of glucose into the inner membrane;

an electrode which oxidizes hydrogen peroxide; and

wherein the inner membrane is disposed between the outer membrane and the electrode.

23. The glucose sensor of claim 22 wherein the outer membrane of the membrane system comprises polycarbonate.

24. The glucose sensor of claim 22 wherein the silicon compound of the enzyme-containing membrane is a cross-linked polyorganosiloxane.

25. The glucose sensor of claim 24 wherein the polyorganosiloxane is polydimethylsiloxane.

26. The glucose sensor of claim 22 wherein the enzyme of the enzyme-containing membrane is one or a combination of enzymes which oxidizes glucose and generates hydrogen peroxide.

27. The glucose sensor of claim 26 wherein the enzyme is an oxidase.

28. The glucose of claim 27 wherein the oxidase is glucose oxidase.

29. The glucose sensor of claim 22 wherein the enzyme is immobilized in the enzyme-containing membrane.

30. The glucose sensor of claim 22 wherein the enzyme is immobilized as an enzyme gel.

31. The glucose sensor of claim 22 wherein the enzyme-containing membrane has a porosity of from about 25% to about 55%.