Method of Identifying a Base in a Nucleic Acid
First Claim
Patent Images
1. A method of identifying a base in a target nucleic acid with a plurality of nucleic acid probes, said probes having fewer bases than said target, comprising the steps of:
- contacting said probes with said target;
identifying a first probe that has the highest binding affinity to said target;
selecting a first set of extension probes comprising at least two of said first probes which are extended differently from each other by the addition at one end of A, C, T, U or G, with or without a deletion at he other end of said extension probes; and
identifying one of said first set of extension probes that hybridizes specifically to said target more strongly than others of said first set of extension probes, whereby said one of said extension probes identifies a base in said target nucleic acid.
3 Assignments
0 Petitions
Accused Products
Abstract
Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. Arrays of nucleic acids are formed by techniques, preferably high resolution, light-directed techniques. Positions of hybridization of a target nucleic acid are determined by, e.g., epifluorescence microscopy. Devices and techniques are proposed to determine the sequence of a target nucleic acid more efficiently and more quickly through such synthesis and detection techniques.
311 Citations
13 Claims
-
1. A method of identifying a base in a target nucleic acid with a plurality of nucleic acid probes, said probes having fewer bases than said target, comprising the steps of:
-
contacting said probes with said target; identifying a first probe that has the highest binding affinity to said target; selecting a first set of extension probes comprising at least two of said first probes which are extended differently from each other by the addition at one end of A, C, T, U or G, with or without a deletion at he other end of said extension probes; and identifying one of said first set of extension probes that hybridizes specifically to said target more strongly than others of said first set of extension probes, whereby said one of said extension probes identifies a base in said target nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
-
-
10. A method of determining if a nucleotide sequence of a target nucleic acid is the same as a sequence of a first nucleic acid comprising:
-
contacting said target nucleic acid with a plurality of nuclei acid probes to determine a core probe subsequence; determining the affinity of said target nucleic acid to probes identical to, but for a single base mismatch, of said core probe subsequence; and determining that said nucleotide sequence of said target is the same as said first nucleic acid if said affinity of said target to probes identical to but for a single base mismatch follows a predetermined pattern. - View Dependent Claims (11, 12, 13)
-
Specification