Antibodies that bind hek ligands

• US 5,969,110 A
• Filed: 04/08/1998
• Issued: 10/19/1999
• Est. Priority Date: 08/20/1993
• Status: Expired due to Fees

• Current Assignee: Immunex Corporation (Amgen Inc.)
• Original Assignee: Immunex Corporation (Amgen Inc.)
• Inventors: Cerretti, Douglas P., Beckmann, M. Patricia
• Primary Examiner(s): MERTZ, PREMA MARIA
• Application Number:
  US09/057,121
• Time in Patent Office: 559 Days
• Field of Search: 530/387.1, 530/389.7, 530/388.1, 530/388.23, 530/388.24, 530/389.1, 530/389.2, 435/325, 435/326, 435/331, 435/335, 514/2, 514/8, 514/12
• US Class Current: 530/387.9
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 35/02 specific for leukemia
• CPC Class Codes: C07K 14/52 Cytokines; Lymphokines; Interferons
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Abstract

Antibodies specific for a hek-L may be generated, using a hek-L polypeptide or fragment thereof as an immunogen. The antibodies may be monoclonal.

Claims

1. A purified antibody that is immunoreactive with a hek ligand (hek-L) protein, wherein the hek-L protein is selected from the group consisting of the protein of SEQ ID NO:

2 and the protein of SEQ ID NO;

4.

2. An antibody according to claim 1, wherein said antibody is a monoclonal antibody that is immunoreactive with the hek-L protein of SEQ ID NO:

2.

3. An antibody according to claim 1, wherein said antibody is a monoclonal antibody that is immunoreactive with the hek-L protein of SEQ ID NO:

4.

4. A purified antibody that is specific for a hek-L protein, wherein said hek-L protein comprises the amino acid sequence presented as residues 1 to 219 of SEQ ID NO:

2.

5. An antibody of claim 4, wherein said antibody is a monoclonal antibody.

6. A purified antibody that is specific for a hek-L protein, wherein said hek-L protein comprises the amino acid sequence presented as residues 1 to 202 of SEQ ID NO:

2.

7. An antibody of claim 6, wherein said antibody is a monoclonal antibody.

8. A purified antibody that is specific for a hek-L protein, wherein said hek-L protein comprises the amino acid sequence presented as residues 1 to 179 of SEQ ID NO:

4.

9. An antibody of claim 8, wherein said antibody is a monoclonal antibody.

10. A purified antibody that is specific for a hek-L protein, wherein said hek-L protein comprises the amino acid sequence presented as residues 1 to 160 of SEQ ID NO:

4.

11. An antibody of claim 10, wherein said antibody is a monoclonal antibody.

12. A method for producing a monoclonal antibody specific for a hek ligand (hek-L) polypeptide, said method comprising the steps of:

a) immunizing an animal with a hek-L polypeptide selected from the group consisting of the protein of SEQ ID NO;

2, an immunogenic fragment of the protein of SEQ ID NO;

2, the protein of SEQ ID NO;

4, and an immunogenic fragment of the protein of SEQ ID NO;

4;

b) harvesting spleen cells from the immunized animal;

c) fusing said spleen cells to a myeloma cell line, thereby generating hybridoma cells;

d) identifying a hybridoma cell line that produces monoclonal antibodies specific for the hek-L polypeptide; and

e) purifying monoclonal antibodies produced by said hybridoma cell line.

13. A method according to claim 12, comprising immunizing the animal with the protein of SEQ ID NO:

2.

14. A method according to claim 12, comprising immunizing the animal with an immunogenic fragment comprising the extracellular domain of the protein of SEQ ID NO:

2.

15. A method according to claim 12, comprising immunizing the animal with the protein of SEQ ID NO:

4.

16. A method according to claim 12, comprising immunizing the animal with an immunogenic fragment comprising the extracellular domain of the protein of SEQ ID NO:

4.

17. A monoclonal antibody produced by a method according to claim 12.

18. A method of producing a hybridoma cell line, said method comprising the steps of:

a) immunizing an animal with a hek-L polypeptide selected from the group consisting of the protein of SEQ ID NO;

2, an immunogenic fragment of the protein of SEQ ID NO;

2, the protein of SEQ ID NO;

4, and an immunogenic fragment of the protein of SEQ ID NO;

4;

b) harvesting spleen cells from said animal;

c) fusing said spleen cells to a myeloma cell line, thereby generating hybridoma cells; and

d) identifying a hybridoma cell line that produces a monoclonal antibody specific for the hek-L polypeptide.

19. A method according to claim 18, comprising immunizing the animal with the protein of SEQ ID NO:

2.

20. A method according to claim 18, comprising immunizing the animal with an immunogenic fragment comprising the extracellular domain of the protein of SEQ ID NO:

2.

21. A method according to claim 18, comprising immunizing the animal with the protein of SEQ ID NO:

4.

22. A method according to claim 18, comprising immunizing the animal with an immunogenic fragment comprising the extraccllular domain of the protein of SEQ ID NO:

4.

23. A method for producing an antibody, comprising immunizing an animal with a hek-L polypeptide selected from the group consisting of the protein of SEQ ID NO:

2, an immunogenic fragment of the protein of SEQ ID NO;

2, the protein of SEQ ID NO;

4, and an immunogenic fragment of the protein of SEQ ID NO;

4;

whereby antibodies directed against the hek-L polypeptide are generated in said animal.