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Electrochemical analyte sensors using thermostable peroxidase

  • US 5,972,199 A
  • Filed: 02/11/1997
  • Issued: 10/26/1999
  • Est. Priority Date: 10/11/1995
  • Status: Expired due to Term
First Claim
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1. An electrochemical sensor, comprising:

  • (a) an electrode;

    (b) a hydrogen peroxide sensing layer on the electrode, comprising a thermostable peroxidase and non-leachable redox centers for transferring electrons between the electrode and the peroxidase; and

    (c) a second enzyme capable of catalyzing the production of hydrogen peroxide in the presence of an analyte, the second enzyme being disposed on the electrode proximate the sensing layer but electrically insulated from the sensing layer;

    wherein the sensor is capable of operation at 37°

    C. with less than 2% loss of sensitivity per hour of continuous operation.

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