Dry solid medium for storage and analysis of genetic material

• US 5,972,386 A
• Filed: 12/19/1995
• Issued: 10/26/1999
• Est. Priority Date: 12/19/1995
• Status: Expired due to Term

• Current Assignee: Whatman Ltd. (United Kingdom) (General Electric Co. (Italy))
• Original Assignee: Flinders Technologies Pty., Ltd.
• Inventors: Burgoyne, Leigh Alexander
• Primary Examiner(s): Myers, Carla J.
• Assistant Examiner(s): JOHANNSEN, DIANA B
• Application Number:
  US08/574,888
• Time in Patent Office: 1,407 Days
• Field of Search: 435/6, 435/91.2, 435/91.5, 435/91.51, 935/77, 424/488, 424/486, 424/489, 424/497, 424/502
• US Class Current: 424/488
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence

Abstract

The present invention provides a dry solid medium for storing a sample of a genetic material, including RNA and DNA, in a form suitable for subsequent analysis. The dry solid medium of the invention includes a dry solid matrix and a composition including a protein denaturing agent, and a chelating agent. The dry solid medium can further include a component which functions in subsequent analysis of the genetic material using, for example, PCR, reverse transcriptase initiated PCR, LCR, RFLP, or genetic hybridization. A component for subsequent analysis includes, for example, a nucleotide sequences such as a primer, DNA or RNA probe, and a target sequence stabilizer. The dry solid medium can also include a retaining agent to enhance retention of a component for subsequent analysis. The invention also provides methods for using the dry solid medium of the invention. The dry solid media and methods of use thereof are particularly suited for use in automated systems.

Claims

1. A dry solid medium for storing at least one sample of genetic material, said dry solid medium comprising:

(a) a solid matrix having sorbed thereto a composition comprising a protein denaturing agent;

(b) a component for subsequent analysis of said sample of genetic material; and

(c) a retaining agent for retaining said component for subsequent analysis on said solid matrix, wherein said retaining agent comprises a hydrophilic solid carrier in wax and wherein said hydrophilic solid carrier comprises a starch, cellulose or cotton material.

2. The dry solid medium according to claim 1, wherein said protein denaturing agent is a detergent.

3. The dry solid medium according to claim 1, wherein said sample of genetic material stored on said dry solid medium is RNA.

4. The dry solid medium according to claim 1, wherein said sample of genetic material stored on said dry solid medium is blood DNA.

5. The dry solid medium according to claim 1, wherein said component for subsequent analysis of said sample of genetic material is a nucleotide sequence.

6. The dry solid medium according to claim 1, wherein said component for subsequent analysis of said sample of genetic material is a target sequence stabilizer.

7. The dry solid medium according to claim 1, wherein said retaining agent reduces loss of said component for subsequent analysis during an aqueous wash procedure.

8. The dry solid medium according to claim 1, wherein said component for subsequent analysis of said sample of genetic material is applied to said dry solid medium after said sample of genetic material is applied to said dry solid medium.

9. The dry solid medium according to claim 1, wherein said dry solid medium is a card comprising more than one component for subsequent analysis.

10. The dry solid medium according to claim 1, wherein said composition of said dry solid medium further comprises a free radical trap.

11. The dry solid medium according to claim 2, wherein said detergent is an anionic detergent.

12. A method for storing and subsequent analysis of a sample of genetic material comprising:

(a) applying a sample of genetic material to a dry solid medium, said dry solid medium comprising;

(i) a solid matrix having sorbed thereto a composition comprising a protein denaturing agent;

(ii) a component for subsequent analysis of said sample of genetic material; and

(iii) a retaining agent for retaining said component for subsequent analysis on said matrix, wherein said retaining agent comprises a hydrophilic solid carrier in wax and wherein said hydrophilic solid carrier comprises a starch, cellulose or cotton material;

(b) storing said sample of genetic material;

(c) washing said sample of genetic material; and

(d) analyzing said sample of genetic material.

13. The method according to claim 12, wherein any of said steps (a), (b), (c) and (d) are performed on an automated system.

14. The method according to claim 12, wherein said sample of genetic material analyzed is RNA which is first transcribed to DNA using reverse transcriptase initiated polymerase chain reaction.

15. The method according to claim 12, wherein said sample of genetic material of step (d) is analyzed using polymerase chain reaction.

16. The method according to claim 12, wherein said washing of said sample of genetic material of step (c) is performed using an aqueous wash system.

17. A method for storing a sample of genetic material comprising:

(a) applying a sample of genetic material to a dry solid medium, said dry solid medium comprising;

(i) a solid matrix having sorbed thereto a composition comprising a protein denaturing agent;

(ii) a component for subsequent analysis of said sample of genetic material; and

(iii) a retaining agent for retaining said component for subsequent analysis on said solid matrix, wherein said retaining agent comprises a hydrophilic solid carrier in wax and wherein said hydrophilic solid carrier comprises a starch, cellulose or cotton material; and

(b) storing said sample of genetic material.