DNA polymerase with modified processivity
First Claim
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1. A chimeric DNA polymerase having a DNA polymerase domain and a processivity factor binding domain not naturally associated with said DNA polymerase domain, wherein said processivity factor binding domain binds thioredoxin.
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Abstract
Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.
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Citations
14 Claims
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1. A chimeric DNA polymerase having a DNA polymerase domain and a processivity factor binding domain not naturally associated with said DNA polymerase domain, wherein said processivity factor binding domain binds thioredoxin.
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2. A chimeric DNA polymerase having a DNA polymerase domain and a processivity factor binding domain not naturally associated with said DNA polymerase domain, wherein said processivity factor binding domain consists essentially of amino acids 236 to 363 from T7 gene 5 protein.
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3. A chimeric DNA polymerase having a DNA polymerase domain and a processivity factor binding domain not naturally associated with said DNA polymerase domain, wherein said polymerase consists essentially of a wild-type DNA polymerase having substituted in a region of the wild-type DNA polymerase corresponding to amino acids 549 to 609 of Klenow fragment of DNA polymerase I, a processivity factor binding domain that corresponds essentially to amino acids 236 to 363 of T7 gene 5 protein.
- 4. A chimeric DNA polymerase having a DNA polymerase domain and a processivity factor binding domain not naturally associated with said DNA polymerase domain, wherein the processivity factor binding domain consists essentially of amino acid residues 258 to 333 of T7 DNA polymerase.
- 8. A processivity factor modified to increase its heat stability.
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12. A chimeric DNA polymerase having a DNA polymerase domain and a processivity factor binding domain not naturally associated with said DNA polymerase domain, wherein said processivity factor binding domain is inserted in place of residues 571 to 577 of E. coli DNA polymerase I.
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13. A chimeric DNA polymerase having a DNA polymerase domain and a processivity factor binding domain not naturally associated with said DNA polymerase domain, wherein said processivity factor binding domain is inserted in place of residues 475 to 481 of Taq DNA polymerase.
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14. A chimeric DNA polymerase having a DNA polymerase domain and a processivity factor binding domain not naturally associated with said DNA polymerase domain, wherein said processivity factor binding domain is inserted in place of residues 477 to 483 of Tth DNA polymerase.
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