×

ATP-dependent protease and use of inhibitors for same in the treatment of cachexia and muscle wasting

  • US 5,972,636 A
  • Filed: 12/02/1997
  • Issued: 10/26/1999
  • Est. Priority Date: 05/13/1991
  • Status: Expired due to Term
First Claim
Patent Images

1. A method of screening for an inhibitor of muscle protein degradation, said method comprising:

  • a) providing cultured cells in which a protein whose degradation is ubiquitin-dependent is produced;

    b) contacting the cultured cells with a substance to be assessed for its ability to inhibit muscle protein degradation, under conditions appropriate for entry of the substance into the cultured cells;

    c) determining the extent to which the protein is present in the cytosol of the cultured cells, wherein accumulation of the protein in the cytosol is indicative that the substance inhibits the ATP-ubiquitin-dependent degradative process; and

    d) identifying a substance that inhibits the ATP-ubiquitin-dependent degradative process as an inhibitor of muscle protein degradation.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×