Fiber-optic imaging probe

• US 5,973,779 A
• Filed: 03/28/1997
• Issued: 10/26/1999
• Est. Priority Date: 03/29/1996
• Status: Expired due to Term

• Current Assignee: Rafat R. Ansari
• Original Assignee: Kwang I. Suh, Rafat R. Ansari
• Inventors: Ansari, Rafat R., Suh, Kwang I.
• Primary Examiner(s): Evans, F. L.
• Application Number:
  US08/828,371
• Time in Patent Office: 942 Days
• Field of Search: 356/301, 356/336, 356/338, 356/342, 356/317, 356/318, 250/458.1, 250/461.1
• US Class Current: 356/301
• CPC Class Codes: A61B 3/117 for examining the anterior chamber or the anterior chamber angle, e.g. gonioscopes
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: G01N 15/0211 Investigating a scatter or diffraction pattern
• CPC Class Codes: G01N 2015/0216 from fluctuations of diffraction pattern

Abstract

A fiber-optic imaging probe is disclosed for use in dynamic light scattering applications. The probe includes two monomode optical fibers and two GRIN lenses to form a pair of identical fiber-lens combinations. Each fiber is positioned off the optical axis of its associated lens. Further, the optical fibers are placed at an image plane of the lenses. The fiber-lens combinations are parallel to and mirror images of each other. The first fiber-lens combination projects a tightly focused spot of light into a scattering volume containing suspended or dispersed microscopic particles, or biological or live tissues. The second fiber-lens combination collects the light which is scattered from the particles in the scattering volume and conveys it to a photo detector and autocorrelator for analysis. The probe minimizes the scattering volume by advantageously using imaging principles of lenses. Further, the probe optimizes sensitivity and spatial coherence. The probe provides rapid determination of particle diffusivities and their respective size distributions in the scattering volume. The probe can be combined with a video and/or microscopic imaging system for visual monitoring of the scattering volume in real time. The probe may be used in a wide variety of optical diagnostic applications. Further, the probe is easy to manufacture and use.

Claims

1. A dynamic light scattering apparatus for analyzing a scattering volume, the apparatus comprising:

a probe body;

an optical arrangement for transmitting coherent light to and receiving scattered light from a scattering volume, the optical arrangement having a first optical fiber and a first lens, the first optical fiber having a first optical fiber end face at a first end portion and a second optical fiber end face, the first optical fiber end face being secured to the probe body, the first lens being optically connected to the first optical fiber and located a first selected distance from the first optical end face to minimize the scattering volume, the lens being secured to the probe body.

2. The apparatus as defined in claim 1 wherein the optical arrangement includes:

a transmitting optical arrangement for transmitting coherent light to the scattering volume, the transmitting optical arrangement including one of the first optical fiber and a second optical fiber, the second optical fiber having a first optical fiber end face at a first end portion and a second optical fiber end face, the first optical fiber end face of the second optical fiber being secured to the probe body; and

a receiving optical arrangement for receiving scattered light from the scattering volume, the receiving optical arrangement including the other of the first optical fiber and the second optical fiber.

3. The apparatus as defined in claim 2 further comprising:

a second lens optically connected to and located a second selected distance from the other of the first optical fiber end faces of the first and second optical fibers to minimize the scattering volume.

4. The apparatus as defined in claim 1 wherein the first end face of the first optical fiber is located at an image plane of the first lens relative to the scattering volume.

5. The apparatus as defined in claim 3 wherein the first and second selected distances are substantially equal.

6. The apparatus as defined in claim 1 wherein the first lens is a GRIN lens.

7. The apparatus as defined in claim 2 wherein the first end portions of the first and second optical fibers are substantially parallel to each other within the probe body.

8. The apparatus as defined in claim 1 wherein the first end portion of the first optical fiber is positioned off the optical axis of the first lens.

9. The apparatus as defined in claim 1 wherein the first optical fiber is a monomide optical fiber.

10. The apparatus as defined in claim 2 further comprising:

a source of coherent light optically connected to the transmitting optical arrangement;

a detector optically connected to the receiving optical arrangement;

a correlator connected to the detector; and

a computer for generating data characteristic of the scattering volume, the computer receiving data from the correlator.

11. The apparatus as defined in claim 10 wherein the detector is a photodiode.

12. The apparatus of claim 1 further comprising:

a visual monitoring system attached to the probe body for viewing the scattering volume.

13. The apparatus of claim 1 wherein:

the end face of the first optical fiber is positioned for a desired penetration depth z₂ a distance z₁ from the first lens having focal length f according to the Gaussian lens equation in order to minimize the scattering volume.

14. A fiber-optic imaging probe for determining a characteristic of a target, the apparatus comprising:

a source of coherent light;

a probe body;

a transmitting lens secured to the probe body;

a receiving lens secured to the probe body;

a transmitting optical fiber for transmitting light to the target, the transmitting optical fiber having first and second end portions, the first end portion secured within the probe body such that it lies off the optical axis of the transmitting lens, the first end portion having an end face, the second end portion connected to the source of coherent light;

a receiving optical fiber for collecting light from the target, the receiving optical fiber having first and second end portions, the first end portion secured substantially parallel to the first end portion of the transmitting optical fiber such that first end portion of the receiving optical fiber lies off the optical axis of the receiving lens, the first end portion of the receiving optical fiber having an end face secured to the probe body, the second end portion of the receiving optical fiber connected to detector.

15. The apparatus of claim 14 wherein one of the transmitting and receiving lenses is a GRIN lens.

16. The apparatus of claim 14 wherein one of the first end faces of the transmitting and receiving optical fibers is located at a selected distance from its associated lens such that the end face is at an image plane of the target.

17. The apparatus of claim 14 wherein one of the transmitting and receiving optical fibers is a monomode optical fiber.

18. A method for examining a scattering volume, the method comprising:

(a) transmitting laser light non linearly from a first optical fiber end face to the scattering volume, the laser light undergoing Gaussian propagation;

(d) receiving back-scattered laser light nonlinearly from the scattering volume to an end face of a second optical fiber;

(e) detecting the scattered light;

(f) converting the detected light into data characteristic of the scattering volume.

19. A method according to claim 18 further comprising:

(g) simultaneously with step (d), monitoring a physical image of the scattering volume.

20. A method according to claim 18 further comprising:

producing a tightly focused scattering volume given a desired scattering angle and a desired penetration depth according to lens equations.

21. A dynamic light scattering (DLS) probe comprising:

means to coherently transmit input light;

means to coherently transmit scattered light;

means to produce a tightly focused scattering volume in a sample with desired scattering angle and penetration depth by utilizing the principles of imaging;

and means to visually monitor the sample in real time.

22. An apparatus according to claim 21 such that the means to coherently transmit input light comprises a monomode optical fiber and the means to coherently transmit scattered light comprises a monomode optical fiber.

23. An apparatus according to claim 21 such that the means to produce a tightly focused scattering volume with desired scattering angle and penetration depth by utilizing the principle of imaging comprises two lenses, each mated with a monomode fiber.

24. An apparatus according to claim 23 characterized in that the two combinations of monomode fiber and lens are housed together in one probe body and they positioned in parallel to each other and mirror imaged.

25. An apparatus according to claim 24 characterized in that for each fiber-lens combination, the end face of the monomode optical fiber is positioned off the optical axis of the lens and away from the lens such that the location of the scattering volume is related to the location of the end face of the monomode fiber and the focal length of the lens according to the Gaussian lens law.

26. An apparatus according to claim 21 such that the means to visually monitor the sample in real time comprises a detachable video microscope.

27. An apparatus according to claim 21 such that the means to visually monitor the sample in real time comprises a detachable fiberscope.

28. An apparatus according to claim 21 used for many diverse dynamic light scattering applications including but not limited to, early diagnosis of various eye diseases (cholesterol deposit and blood sugar level in anterior chamber, cataract in the lens, diabetic retinopathy and age related molecular change in the vitreous), monitoring protein crystallization process, study of polymer induced flocculation and aggregation, study of highly concentrated and interacting systems, characterization of food protein, monitoring of the synthesis of microporous material (zeolite crystal), and skin/tissue analysis.

29. An apparatus according to claim 21 to be used for other optical diagnostics techniques including static light scattering, Brillouin scattering, Raman scattering, laser Doppler velocimetry, fluorescence spectroscopy, and transmission and absorption spectroscopy.