Sample wash station assembly

• US 5,976,470 A
• Filed: 05/29/1998
• Issued: 11/02/1999
• Est. Priority Date: 05/29/1998
• Status: Expired due to Fees

• Current Assignee: Ontogen Corporation (Infinity Pharmaceuticals, Inc.)
• Original Assignee: Ontogen Corporation (Infinity Pharmaceuticals, Inc.)
• Inventors: Maiefski, Romaine, Wendell, Don
• Primary Examiner(s): Le, Long V.
• Application Number:
  US09/087,376
• Time in Patent Office: 522 Days
• Field of Search: 422/63, 422/65, 422/68.1, 422/81, 422/100, 422/101, 422/103, 422/104, 436/43, 436/49, 436/174, 436/179, 436/180, 222/144.5, 222/137, 222/330, 222/485
• US Class Current: 422/501
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00313 the reactor vessels being formed by arrays of wells in blocks
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00373 Hollow needles
• CPC Class Codes: B01J 2219/00376 in multiple or parallel arrangements
• CPC Class Codes: B01J 2219/00389 Feeding through valves
• CPC Class Codes: B01J 2219/00391 Rotary valves
• CPC Class Codes: B01J 2219/00416 Vacuum
• CPC Class Codes: B01J 2219/00418 using pressure
• CPC Class Codes: B01J 2219/00484 by shaking, vibrating or oscillating of the reaction vessels
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: G01N 35/1067 for transfer to or from containers having different spacing
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A wash station assembly and method for washing selected samples in a sample containing assembly. The wash station assembly includes a wash station having a frame and a fluid dispensing assembly connected to the frame. The fluid dispensing assembly has a selector valve that is connectable to a plurality of fluid lines and is adjustable to allow only one of the fluids to pass through the selector valve at a time. An array of syringes is coupled to the selector valve and positioned to receive the fluid passing through the selector valve. Each syringe includes a check valve that prevents backflow of fluid or air into the syringe through the pipetting needles. A distribution manifold is connected to the selector valve and receives the fluid passing through the selector valve. The manifold has a plurality of distribution channels coupled to the syringes, to direct the fluid into the syringes. Each distribution channel has a manifold valve therein that prevents backflow of fluid into the manifold from the syringes. An array of pipetting needles is connected to the syringes and is positioned to direct the fluid into the sample containing assembly for washing the samples. The pipetting needles have a radially directed opening in the distal end to direct the fluid radially away from the needle during the dispensing process. A waste management system is connected to the wash station to automatically separate halogenated waste fluids from non-halogenated waste fluids.

Claims

1. A fluid dispensing assembly for dispensing a selected fluid into multiple vessels, the fluid dispensing assembly being connectable to a fluid source, comprising:

a distribution manifold having a manifold inlet positioned to receive fluid from the fluid source, the distribution manifold having a plurality of distribution channels coupled to the manifold inlet, the distribution channels each having a separate channel outlet, each distribution channel having a first valve therein to allow the fluid to flow in only one direction in the respective distribution channel; and

an array of fluid dispensers connected to the distribution manifold, each fluid dispenser being connected to the channel outlet of a respective distribution channel to receive the fluid passing through the channel outlet, each fluid dispenser having a second valve therein to allow the fluid to flow in only one direction out of the respective fluid dispenser.

2. The fluid dispensing assembly of claim 1 further comprising a gate valve connected to the distribution manifold and positioned to control fluid flow from the fluid source to the distribution manifold.

3. The fluid dispensing assembly of claim 1 having a plurality of fluid sources with fluids therein, the assembly further comprising a selector valve connectable to the plurality of fluid sources, the selector valve being adjustable to one of a plurality of positions to allow a selected one of the fluids to pass therethrough to the distribution manifold.

4. The fluid dispensing assembly of claim 3 wherein the selector valve includes a body with a plurality of fluid inlets connectable fluid lines from the fluid sources, a plurality of fluid passageways in the body communicating with the fluid inlets, a valve member connected to the body, and an outlet channel communicating with the valve member and the manifold inlet, the valve member having a valve channel therethrough that communicates with the outlet passageway, the valve member being adjustable to position the valve channel in communication with one of the fluid passageways to direct fluid from the one of the fluid passageways into the outlet passageway and into the manifold inlet and the valve member blocks the other fluid passageways to prevent fluid from flowing therethrough.

5. The fluid dispensing assembly of claim 4 wherein the selector valve assembly has a gate valve in the outlet passageway, the gate valve being adjustable between open and closed positions to control the flow of fluid through the outlet passageway.

6. The fluid dispensing assembly of claim 4 wherein the valve member is a rotary valve member that rotationally adjustable to separately align the valve channel with each of the fluid passageways.

7. The fluid dispensing assembly of claim 4 wherein the selector valve includes a valve control assembly connected to the valve member and being adjustable to move the valve member to align the valve channel with the selected one of the fluid passageways.

8. The fluid dispensing assembly of claim 7, further comprising a programmable controller connected to the valve control assembly for controlling adjustment of the valve member relative to the fluid passageways.

9. The fluid dispensing assembly of claim 4, further comprising a programmable controller and the selector valve having a control device operatively connected to the controller for automatic adjustment of the valve member relative to the fluid passageways.

10. The fluid dispensing assembly of claim 1 wherein the fluid dispensers are syringes.

11. The fluid dispensing assembly of claim 1 wherein the array of fluid dispensers include 96 syringes.

12. The fluid dispensing assembly of claim 1 further comprising distributor members connected to the fluid dispensers, the distributor members having an interior passageway, a distal end portion, and a radially directed aperture in the distal end portion, the aperture communicating with the interior passageway to direct a flow of fluid radially away from the distal end portion.

13. The fluid dispensing assembly of claim 1, further including a frame, a distributor support connected to the frame and the distribution manifold, and a drive mechanism connected to the distributor support, the distributor support interconnects the distribution manifold to the frame, the drive mechanism being movable to move the distribution manifold and fluid dispensers as a unit relative to the frame between raised and lowered positions.

14. The fluid dispensing assembly of claim 1 wherein the distribution manifold has only one manifold inlet in fluid connection with each distribution channel.