Chimeric kidney

• US 5,976,524 A
• Filed: 02/11/1997
• Issued: 11/02/1999
• Est. Priority Date: 01/06/1997
• Status: Expired due to Term

• Current Assignee: Washington University In St Louis
• Original Assignee: Washington University In St Louis
• Inventors: Hammerman, Marc
• Primary Examiner(s): Stanton, Brian R.
• Assistant Examiner(s): HAUDA, KAREN MARIE
• Application Number:
  US08/797,201
• Time in Patent Office: 994 Days
• Field of Search: 424/93.1, 424/93.21, 424/9.2, 424/9.1, 435/375
• US Class Current: 424/93.1
• CPC Class Codes: A61K 35/22 Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland
• CPC Class Codes: A61K 38/30 Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2 insulin-like growth factor binding protein A61K38/1754
• CPC Class Codes: A61P 13/00 Drugs for disorders of the urinary system

Abstract

Methods for increasing the nephron mass of a mammalian recipient are disclosed. A metanephros from an allogenic or xenogeneic mammalian donor is implanted next to a recipient'"'"'s omentum or under the renal capsule of the recipient'"'"'s kidney. The metanephros becomes vascularized by the recipient'"'"'s blood vessels, forming a chimeric kidney that produces urine and develops a ureter that facilitates externalization of the urine. A ureter to ureter anastomosis can be subsequently performed to provide fluid communication between the chimeric kidney ureter and a ureter of the recipient.

Claims

1. A method of increasing the nephron mass of a mammalian recipient comprising implanting at least one whole metanephros of an embryonic mammalian donor next to the recipient'"'"'s omentum or under the renal capsule of the recipient'"'"'s kidney under conditions that allow the metanephros to become vascularized, forming a chimeric kidney that produces urine and develops a ureter that facilitates externalization of the urine.

2. The method of claim 1 wherein the metanephros is implanted next to the recipient'"'"'s omentum.

3. The method of claim 2 wherein the metanephros is implanted into an omental fold near the recipient'"'"'s kidney.

4. The method of claim 1 wherein the metanephros is implanted under the renal capsule of the recipient'"'"'s kidney.

5. The method of claim 1 wherein the metanephros has an intact renal capsule.

6. The method of claim 1 wherein at least two whole metanephroi with renal capsules intact are implanted into the recipient.

7. The method of claim 1 wherein the metanephros is allogeneic to the recipient.

8. The method of claim 1 wherein the embryonic donor is xenogeneic to the recipient.

9. The method of claim 8 further comprising immunosuppressing the recipient.

10. The method of claim 1 wherein the metanephros is obtained from the donor within 2 to 4 days after embryonic development of the metanephros begins.

11. The method of claim 1 wherein the metanephric tissue is obtained from the donor prior to the presence of blood vessels within the metanephric tissue.

12. The method of claim 1 wherein the recipient has reduced functional renal mass prior to implantation of the metanephros.

13. The method of claim 1 wherein after the chimeric kidney ureter develops, a ureter to ureter anastomosis is performed to provide fluid communication between the chimeric kidney ureter and a ureter of the recipient.

14. The method of claim 1 wherein the mammalian recipient is a juvenile or adult.

15. The method of claim 1 wherein the metanephros is implanted within one hour after removal from the embryonic donor.

16. The method of claim 1 wherein prior to implantation of the metanephros, renal tissue is removed from the mammalian recipient.