Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer

• US 5,976,567 A
• Filed: 06/06/1996
• Issued: 11/02/1999
• Est. Priority Date: 06/07/1995
• Status: Expired due to Term

• Current Assignee: Tekmira Pharmaceuticals Corporation, University of British Columbia
• Original Assignee: Inex Pharmaceuticals Corporation
• Inventors: Scherrer, Peter, Cullis, Pieter R., Wheeler, Jeffery J., Bally, Marcel B., Zhang, Yuan-Peng, Reimer, Dorothy L., Hope, Michael
• Primary Examiner(s): Degen, Nancy
• Assistant Examiner(s): LARSON, THOMAS GEORGE
• Application Number:
  US08/660,025
• Time in Patent Office: 1,244 Days
• Field of Search: 514/44, 514/2, 424/450, 435/172.3, 435/458, 935/54
• US Class Current: 424/450
• CPC Class Codes: A61K 47/6911 the form being a liposome
• CPC Class Codes: A61K 9/1272 with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids with cholesterol as the only non-phosphatidyl surfactant A61K9/127; lipids as modifying agent A61K47/543
• CPC Class Codes: C12N 15/88 using microencapsulation, e.g. using amphiphile liposome vesicle

Abstract

Novel lipid-nucleic acid particulate complexes which are useful for in vitro or in vivo gene transfer are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. Upon removal of a solubilizing component (i.e., detergent or an organic solvent) the lipid-nucleic acid complexes form particles wherein the nucleic acid is serum-stable and is protected from degradation. The particles thus formed have access to extravascular sites and target cell populations and are suitable for the therapeutic delivery of nucleic acids.

Claims

1. A method for the preparation of lipid-nucleic acid particles, comprising:

(a) contacting nucleic acids with a solution comprising non-cationic lipids and a detergent to form a nucleic acid-lipid mixture;

(b) contacting cationic lipids with said nucleic acid-lipid mixture to neutralize the negative charge of said nucleic acids and form a charge-neutralized mixture comprising detergent, nucleic acids and lipids; and

(c) removing said detergent from said charge-neutralized mixture to provide said lipid-nucleic acid particles in which said nucleic acids are protected from degradation.

2. The method in accordance with claim 1, wherein said solution of step (a) further comprises an organic solvent.

3. The method in accordance with claim 1, wherein said cationic lipids are members selected from the group consisting of DODAC, DDAB, DOTMA, DOSPA, DMRIE, DOGS and combinations thereof.

4. The method in accordance with claim 1, wherein said non-cationic lipids are selected from the group consisting of ESM, DOPE, polyethylene glycol-based polymers and combinations thereof.

5. The method in accordance with claim 1, wherein said detergent is octyl-β

-D-glucopyranoside, said cationic lipid is DODAC, said non-cationic lipid is ESM, and said detergent is removed by dialysis.

6. The method in accordance with claim 5, wherein said non-cationic lipids are combinations of ESM and PEG-Ceramide.

7. The lipid-nucleic acid particle prepared according to claim 1.

8. A method for the preparation of lipid-nucleic acid particles, comprising:

(a) contacting an amount of cationic lipids with nucleic acids in a solution;

said solution comprising of from about 15-35% water and about 65-85% organic solvent and said amount of cationic lipids being sufficient to produce a +/- charge ratio of from about 0.85 to about 2.0, to provide a hydrophobic, charge-neutralized lipid-nucleic acid complex;

(b) contacting said hydrophobic lipid-nucleic acid complex in solution with non-cationic lipids, to provide a lipid-nucleic acid mixture; and

(c) removing said organic solvents from said mixture to provide said lipid-nucleic acid particles in which said nucleic acids are protected from degradation.

9. The method in accordance with claim 8, wherein said cationic lipids are members selected from the group consisting of DODAC, DDAB, DOTMA, DOSPA, DMRIE, DOGS and combinations thereof.

10. The method in accordance with claim 8, wherein said non-cationic lipids are members selected from the group consisting of ESM, DOPE, polyethylene glycol-based polymers and combinations thereof.

11. The method in accordance with claim 8, wherein said organic solvents are members selected from the group consisting of methanol, chloroform, methylene chloride, ethanol, diethyl ether and combinations thereof.

12. The method in accordance with claim 8, wherein said nucleic acid is a plasmid, said cationic lipid is a member selected from the group consisting of DODAC, DDAB, DOTMA, DOSPA, DMRIE, DOGS and combinations thereof said non-cationic lipid is a member selected from the group consisting of ESM, DOPE, polyethylene glycol-based polymers and combinations thereof, and said organic solvent is a member selected from the group consisting of methanol, chloroform, methylene chloride, ethanol, diethyl ether and combinations thereof.

13. The lipid-nucleic acid particle prepared according to claim 8.

14. The method for the preparation of serum-stable plasmid-lipid particles, comprising:

(a) combining a plasmid with cationic lipids in a detergent solution to provide a coated plasmid-lipid complex;

(b) contacting non-cationic lipids with said coated plasmid-lipicl complex to provide a solution comprising detergent, a plasmid-lipid complex and non-cationic lipids; and

(c) removing said detergent from said solution of step (b) to provide a solution of serum-stable plasmid-lipid particles, wherein said plasmid is encapsulated in a lipid bilayer and said particles are serum-stable and have a size of from about 50 to about 150 nm.

15. The method in accordance with claim 14, wherein said removing is by dialysis.

16. The method in accordance with claim 14, wherein step (b) further comprises adding a polyethylene glycol-lipid conjugate.

17. The method in accordance with claim 14, wherein said polyethylene glycol-lipid conjugate is a PEG-ceramide conjugate.

18. The method in accordance with claim 14, further comprising;

(d) sizing said particles to achieve a uniform particle size.

19. The method in accordance with claim 14, wherein said cationic lipids are selected from the group consisting of DODAC, DDAB, DOTAP, DOTMA, DOSPA, DOGS, DC-Chol and combinations thereof.

20. The method in accordance with claim 14, wherein said non-cationic lipids are selected from the group consisting of DOPE, POPC, EPC and combinations thereof.

21. The method in accordance with claim 14, wherein said detergent solution comprises a detergent having a critical micelle concentration of between about 20 mM and 50 mM.

22. The method in accordance with claim 21, wherein said detergent is n-octyl-β

-D-glucopyranoside.

23. The plasmid-lipid particle prepared according to claim 14.

24. A method for the preparation of serum-stable plasmid-lipid particles, comprising;

(a) preparing a mixture comprising cationic lipids and non-cationic lipids in an organic solvent;

(b) contacting an aqueous solution of plasmid with said mixture prepared in step (a) to provide a clear single phase; and

(c) removing said organic solvent to provide a suspension of plasmid-lipid particles, wherein said plasmid is encapsulated in a lipid bilayer, and said particles are stable in serum and have a size of from about 50 to about 150 nm.

25. The method in accordance with claim 24, wherein said non-cationic lipids comprise a polyethylene glycol-lipid conjugate.

26. The method in accordance with claim 25, wherein said polyethylene glycol-lipid conjugate is a PEG-ceramide conjugate.

27. The method in accordance with claim 24, wherein said cationic lipids are selected from the group consisting of DODAC, DDAB, DOTAP, DOTMA, DOSPA, DOGS, DC-Chol and combinations thereof.

28. The method in accordance with claim 24, wherein said nori-cationic lipids are selected from the group consisting of DOPE, POPC, EPC and combinations thereof.

29. The plasmid-lipid particle prepared according to claim 24.