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Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer

  • US 5,976,567 A
  • Filed: 06/06/1996
  • Issued: 11/02/1999
  • Est. Priority Date: 06/07/1995
  • Status: Expired due to Term
First Claim
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1. A method for the preparation of lipid-nucleic acid particles, comprising:

  • (a) contacting nucleic acids with a solution comprising non-cationic lipids and a detergent to form a nucleic acid-lipid mixture;

    (b) contacting cationic lipids with said nucleic acid-lipid mixture to neutralize the negative charge of said nucleic acids and form a charge-neutralized mixture comprising detergent, nucleic acids and lipids; and

    (c) removing said detergent from said charge-neutralized mixture to provide said lipid-nucleic acid particles in which said nucleic acids are protected from degradation.

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